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杂交

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The Genetic variations of wheat line SY95-71 in comparison with its parents, a hexaploid triticale Eronga 83 and wheat line Fan6, were investigated by seed storage protein electrophoresis.

对以六倍体小黑麦Eronga 83和普通小麦品种繁6杂交培育的小麦品系SY95-71及其亲本进行了种子贮藏蛋白电泳分析。

The result implied that the resistance of preharvest sprouting in synthetic hexaploidy wheat could be transferred to common wheat by crossing.

这一研究结论清楚地表明,人工合成小麦穗发芽抗性是可以通过杂交转移到普通小麦中来的。

A synthetic hexaploidy wheat with resistant to preharvest sprout and hard glume was employed to cross with a common wheat line with character of susceptible to preharvest sprout and normal glume hardness, and then built up the F3 population, aiming at studying the relationship between the resistance to preharvest sprout and its glume hardness.

本研究利用抗穗发芽、硬颖壳的人工合成小麦与正常颖壳硬度、穗发芽的普通小麦杂交构建的杂种F3代群体,研究穗发芽与颖壳硬度之间的关系。

Whole-mount in situ hybridization showed that zebrsfish rnf141 gene expressed ubiquitously during embryogenesis, with higher expression found in the telencephalon, cerebellum and hindbrain.

杂交结果显示,rnf141基因在斑马鱼胚胎中广泛表达,尤以端脑、小脑和后脑表达丰度为高。

The result of WISH showed that the trascription of hira gene distributed widely in the early stages of embryos development and centralized to head, heart and the end of tail. Hira gene might affect the development of brain and heart. The expression characterization of colored crucian carp Cahira gene in different tissues and different stages of embryogenesis were also investigated by RT-PCR.

胚胎原位杂交显示,Hira基因的转录本在银鲫胚胎早期广泛分布,而在胚胎后期主要集中于头部、心脏及尾芽尖部,说明也对其脑部和心脏的发育有影响。

In situ hybridization histochemistry and Immunohistochemistry technique were applied to the detection of CD14 in dental tissue.

原位杂交和免疫组织化学研究提示CD14在牙髓炎、牙龈炎中有重要作用。

We detected the expression of VEGF mRNA by in situ hybridization and Ki 67 protein by immune histochemistry.

原位杂交法检测瘤组织血管内皮生长因子mRNA表达;免疫组化法检测瘤组织中细胞增殖核抗原Ki 67的表达。

(1) In our research,frequent expression of BSAP in H/RS cell of classical Hodgkins disease provides further evidence for its B-cell origin,and the expression of BSAP on H/RS cells also can be in favor of the identification of H/RS cells and can distinguish HL from ALCL.(2) Expression of BSAP is located in nucleoli of cells,while that of CD20 in membrane.

结果 (1)33例cHL中,30/33(90.91%)H/RS细胞和33/33(100%)背景淋巴细胞BSAP表达阳性,而H/RS细胞10/33(30.30%)CD20阳性,1/33(3.03%)CD45RO阳性,31/33(93.94%)CD30阳性,25/33(75.75%)CD15阳性;(2)33例HL石蜡刮片组织中有16例(48.48%)出现IgH基因重排阳性,显微切割的19管H/RS细胞有14管(73.68%)扩增阳性,而12管背景淋巴细胞有2管(16.67%)扩增阳性;(3)测序分析周新华第一军医大学病理学教研室获得了一段免疫球蛋白重链可变区的重排片断;(4)原位PCR结果发现杂交的阳性颗粒不仅出现在探针来源的同一病例也出现在不同病例的HillS细胞核上,同时在部分周围背景淋巴细胞核上也见到阳性颗粒。

The movement of a Holliday junction that causes different hybridizations between the homologous chromosomes.

指Holliday交叉的移动,它会引起同源染色体不同区段之间发生杂交

Recent african origin of modern humans revealed by complete sequences of hominoid mitochondrial DNA s.

同时在本实验中,20个差异显示带经Northern杂交获6个阳性克隆,占30%,这与文献报道〔8,9〕的机率相符。

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