杂交

Wirehair Dachshund: Wirehair dachshunds were developed by breeding smooth Dachshunds with various hard-coated terriers and wire-haired pinschers.

刚毛腊肠：刚毛腊肠的发展是短毛腊肠和不同的硬毛的梗和刚毛pinschers杂交而成。

We will continue to flourish because our diverse American society has the strength, hardiness, and resilience of the hybrid plant we are.

我们会继续繁荣昌盛，因为我们是一株杂交植物，我们多元的美国社会有这一植物的力量、坚韧、适应力。

The result suggested that in all lines sellected from semi-exotic populations constructed by crossing CIMMYT germplasm Pob46 and Pob45 with lines in Heilongjiang province, more than a half of them were middle resistant to head smut and the high resistance accounted for 35.9%.

结果表明在用CIMMYT种质Pob45和Pob46与黑龙江省部分自交系杂交构建的半外来群体的选系中，对丝黑穗病中抗及其以上的选系占一半以上，其中高抗占35.9%。

Materials and Methods: Forty Sprague-Dawley rats were randomly divided into normal group, control operative group and operative group which includes 6 subgroups (1,3,7,14,21,28 days). The spinal cord hemisection injury model was made by cutting the posterior right side spinal cord. Function changes in the right posterior limb were evaluated by BBB method. The general morphology changes were observed by HE stain.

材料与方法：40只成年SD大鼠随机分为正常组，假手术组和手术组，手术组又分成1，3，7，14，21，28天组，选取制作脊髓右侧半横断损伤模型，BBB法进行右下肢功能评分，HE染色观察损伤脊髓不同时段的大体形态学变化，原位杂交法观察slit-2 mRNA的表达变化，计算阳性细胞灰度值，进行统计学分析。

The latter observation also suggests that MOC1 promotes the outgrowth of tiller buds besides its promotive effect on axillary meristem initiation and tiller bud formation. MOC1 protein belongs to the GRAS transcription factor family, with two leucine heptad repeats, a VHIID domain, and an SH2-like domain.

MOC1基因的组织原位杂交实验表明，在腋生分生组织形成之前，MOC1基因就已经在叶腋里的少数表皮或亚表皮细胞里表达，并且MOC1基因的表达伴随着腋生分生组织形态建成的整个过程；在腋生分生组织分化形成分蘖芽以后，MOC1仍然在整个分蘖芽中表达。

The present invention selects and uses hereditary hypertension rat and rat with normal blood pressure to make hybridization, and the rat whose blood pressure is normal and arterial pressure susceptivity reflection sensitivity is less than 0.3 ms/mm Hg can be screened out from filial generation, bred and propagated, and repeatedly bred and propagated, after above 12 generations so as to obtain the invented pure rat with stable hereditary property and spontaneous arterial pressure susceptivity reflection functional defect.

本发明涉及医学实验动物模型技术领域，是培育用于心血管疾病防治研究的一种自发性动脉压力感受性反射功能缺陷的纯种大鼠的方法。本发明选用遗传性高血压大鼠和正常血压大鼠杂交，从子代中筛选出血压正常、动脉压力感受性反射敏感性低于0.3ms/mmHg的大鼠培育繁殖，并反复按此标准在其下一代中筛选、繁殖，经12代以上便得到遗传性稳定的自发性动脉压力感受性反射功能缺陷纯种大鼠。

Based on the biochemical analysis of the two proteins in vitro, the results of yeast two-hybrid experiment and protein-interaction in both wild type cells and C48 strain, we conclude HetR can form a homodimer through the Cys48 residue. We also show that the dimerization is required for heterocyst differentiation.

基于体外两种蛋白的性质比较和酵母双杂交的结果，并结合相应蓝细菌野生型和突变株C48A中HetR蛋白性质的体内研究表明：HetR蛋白可以通过第48位半胱氨酸残基形成共价结合的同源二聚体，该同源二聚体是异型胞正常分化所必需的。

Immunofluorescence microscopy may be used to follow the movement of two proteins from different cells following fusion of the cells to form a hybrid heterokaryon.

免疫荧光显微术可用来观察两种细胞融合后形成的杂交异核体中来源不同的两种蛋白质的运动。

Immediately after fusion the two integual proteins are found segregated at either end of the heterokaryon but with time diffuse to all areas of the cell surface .

刚融合时，两种内在蛋白分居在异核体两侧，但随着时间的推移，即扩散到整个杂交细胞表面。

After that, we detect cell DNA heteroploid and cell cycle transformation of human osteosarcoma tissue by using flow cytometry method; next, we amplification the exon2 and exon3 of 〓 gene in 36 cases human osteosarcoma tissue, and amplification products were performed the sepharose and polyacrylamide gel electrophoresis immediately.

然后采用原位杂交方法，对45例骨肉瘤标本组织和10例骨纤维结构不良组织中〓基因mRNA进行检测，以了解骨肉瘤标本组织和骨纤维结构不良组织中〓基因在蛋白质水平和mRNA水平的表达情况。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！