杂交

Found a different cDNA library with Malasesiza furfur in hypha phase by Subtractive Hybridization; And sequencing some fragment of differential expression gene. Provid foundation for the further study on complete differential gene expression.

采用消减杂交技术建立了糠秕马拉色菌的差异cDNA文库；并对部分克隆片段进行测序，为今后获得完整的差异表达基因打下基础。

First, to isolate the total RNA both in hypha and yeast phase of Malasesiza furfur, Then reverse them to cDNA and found a different cDNA library with Malasesiza furfur in hypha phase by Subtractive Hybridization; At last, clone and sequencing the gene fragment on differential expression of Malasesiza furfur in hypha phase.

首先提取糠秕马拉色菌两相细胞的总RNA；然后逆转录生成两相细胞cDNA，运用消减杂交技术获得菌丝相细胞差异表达的cDNA，并建立差异文库：最后对差异cDNA片段克隆测序。

Fusarium oxysporum from wilt diseases in Guangxi hybrid bamboo were separated and purified, and the biological characteristics of the pathogen were studied.

分离纯化了广西杂交竹枯萎病原镰刀属尖孢镰刀菌，并研究了该病原菌的生物学特性。

Via estimating and choosing the offspring of Gerbera jamesonii ,six relatively good individuals had been chosen out .

另外通过对杂交后代的评比和选择，选育了6个综合性状较好的优良新品系。

When the gerbera was first hybridized, delicate [2] pink, yellow and peach dominated the color selection.

花农最初将大丁草与其它花种杂交的时候，可供挑选的颜色主要是浅粉色、浅黄色以及浅桃红。

Up-regulation of Hsp70 protein is consistent with germ cell apoptosis increase.

原位杂交结果显示的mRNA转录水平的变化同蛋白水平的变化一致。

Fluorescence in situ Hybridization ;β E globin gene ; transgenic mice ; integration site.

荧光原位杂交技术；人βE珠蛋白基因；转基因小鼠；整合位点

This research result is used as the reference for choosing parents in breeding new conventional rice and hybrid rice containing higher glutelin content.

开展本试验可为今后利用杂交方法培育高谷蛋白常规水稻以及高谷蛋白杂交稻组合研究中的亲本选择提供参考。

Grouped by factor of smoking, goblet cell hyperplasia had significant differences between smoking and non-smoking subjects.

ICAM-1的原位杂交和免疫组化阳性染色在基底膜和上皮细胞顶端最明显，但表达密度和强度存在差异。

Results ITF and ITF mRNA were expressed in whole small intestine and most of them were localized in goblet cell of intestinal villus.

在此基础上采用原位杂交、免疫组化和高效液相色谱等手段观察了烧伤后两组大鼠ITF 及ITF mRNA的变化。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！