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杂交

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Methods mRNA of placenta from normal pregnancy and PIH were extracted and labeled with flourescent Cy3 and Cy5 in reverse transcription,respectively.The Biodoor chip was hybridized with labeled cDNA.

方法从正常妊娠胎盘和PIH胎盘中抽提mRNA,反转录后,分别用Cy3、Cy5荧光标记,获得两组胎盘来源的cDNA探针。cDNA探针与含8 190条人cDNA点样的BioDoor基因芯片杂交,结果由激光共聚焦扫描仪扫描,并用软件进行统计分析。

Fluorescence in situ hybridization has become one of the important techniques in both medical research and clinical diagnosis.

荧光原位杂交已成为医学遗传学领域中一项极为重要的研究手段。

Pingbianensis flowers were ants (Aphaenogaster smythiesii Forel,Formicidea), springtail (Hypogastrura sp., Hypogastruridae, Collembola) and one species of beetles (Anomala corpulenta Motsch, Rutelidae). These pollinators generally have restricted movement capacities and hence promote geitonogamy or mating between individuals in close proximity within populations. The results of out crossing index pollination experiments in our study suggest that T. pingbianensis has an animal-pollinated, mixed selfing and outcrossing breeding systems.

通过对屏边开口箭的开花物候、传粉生物学、交配系统的研究以及对近交衰退的检测,结果显示:屏边开口箭单花花期约15天左右,柱头、花粉活力持续时间较长;传粉昆虫多为蚂蚁、跳虫,偶有小甲虫拜访,缺乏长距离传粉昆虫;杂交指数以及套袋试验的结果表明屏边开口箭为高度自交亲和的混交模式植物,且在幼苗早期并没有表现出明显的近交衰退现象。

Results Hybridization for AU126 probe was performed with 10% formamide in the hybridization buffer.

结果 AU126探针理想杂交是在10%甲酰胺中进行。

By fluorescence in situ hybridization. Strong fluorescence signals with the probes were observed in 20%~60% formamide concentrations.

的16S rRNA基因序列,设计荧光原位杂交检测博德特氏菌的寡核苷酸探针FW_iso_62和FW_iso_761,在20%~60%甲酰胺均有很强的荧光信号。

Freesia ; New type ; Hybird ; Radiation ; New cultivar

小苍兰;新类型;杂交;辐射;新品种

We can still find some varieties with frilly petals today, but they are hybrids.

今天我们仍然可以看到很多带褶皱花瓣的郁金香变种,但它们都是杂交的。

Southern blot analysis revealed distribution of these sequences in Fritillaria ussuriensis.

Southern杂交结果显示了各组序列在平贝母基因组中的分布情况。

Southern blot analysis revealed distribution of these sequences in Fritillaria ussuriensis. Maxim.

Southern杂交结果显示了各组序列在平贝母基因组中的分布情况。

Expression of islet-1 in spinal cord of adult rat.In grey of the spinal cord of adult rat, the results of both of ISL1 immunohistochemistry and islet-1 mRNA in situ hybridi-zation showed that islet-1 gene expressed predominantly in the nuclei of motoneurons in ventral region and some cell around central canal and dorsal region. In white of spinal cord, the expression of islet-1 located in astroglia in anterior and dorsal funiculus.

在正常成年大鼠脊髓灰质中,ISL-l免疫组化和islet-lmRNA原位分子杂交结果都显示islet-1在腹侧角运动神经元中有相当强度的表达;在中央管周围和背侧角胶状质也有islet-1阳性表达细胞存在;在脊髓白质中,islet-l在腹侧索和背侧索中的星形胶质细胞有表达,外侧索相对较少。

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