杂交

In my experimentⅠcompare the purposes of different combinations of endoenzyme(MseⅠ-HindⅢ, MseⅠ-PstⅠ, MseⅠ-EcoRⅠ) and find that MseⅠ-HindⅢeasily finds thepolymorphism by primers combinations suiTab for AFLP analysis.And by means of this there aremany polymorphism strips. So the double endoenzyme of AFLP was exercisedfor the analysis of soybean cytoplasmic male sterility.In the cource of my experiment, 64 primerpairs of MseⅠand HindⅢwere exerted to analyse the twe mtDNA pools which are made ofsterilities and holdings and the polymorphisms of sterilities and holdings.

运用扩增片段长度多态性的方法，对独特的杂交大豆试材进行分析研究，通过比较MseⅠ-HindⅢ、MseⅠ-PstⅠ、MseⅠ-EcoRⅠ不同酶切组合的效果，结果表明MseⅠ-HindⅢ较容易找到多态性引物组合，而且具有较高的多态性，本试验选用MseⅠ-HindⅢ进行双酶切大豆线粒体DNA从而完成AFLP的分析工作，试验用64对MseⅠ和HindⅢ引物对两个不育系及保持系材料的线粒体DNA制成的pool进行AFLP标记分析，分析了不育系与保持系之间的多态性； 4。

In situ hybridization showed that CYP11B2 mRNA was localized in the endoplasm of liver fat -storing cell and typeⅡ alveolar cells of the lung .

结果 用RT-PCR及Southern Blot法证明了肝脏和肺脏能表达CYP11B2，原位杂交表明CYP11B2 mRNA定位在肝脏的伊藤细胞及肺脏的Ⅱ型肺泡上皮细胞的胞浆内。

After thermal induction, no specific recombinant protein band in SDS-PAGE was found, but G-CSF activity was detectable. Therefore, a new recombinant plasmid pBVHG2 expressing hG-CSF hybrid protein with additional 8 amino acids which could be cut off specifically with the help of mucosal enterokinase at the N terminus of hG-CSF was constructed.

含此质粒菌株虽然表达菌体裂解后可测得明显的生物学活性，但SDS-PAGE仍未见特异表达产物带；因此，再应用相同方法，在hG-CSF cDNA突变体5′端增加24核苷酸对的FLAG肽编码序列，构建了hG-CSF杂交蛋白（hG-CSF天然蛋白N末端增加8aa的FLAG肽，后者可由肠激肽酶切除）的表达质粒pBVHG2。

Methods:CBFβ-MYH11 fusion transcripts and inv (16) were analyzed by reverse-transcriptase polymerase chain reaction and fluorescence in situ hybridization,respectively,in acute myelomonocytic leukemia(M4) with or without eosinophilia.

用逆转录-聚合酶链反应和荧光原位杂交技术分别检测急性粒-单核细胞白血病(M4)患者的CBFβ-MYH11融合基因转录本和inv(16)。

In zebrafish, the expression of fez becomes detectable at the anterior edge of the presumptive neuroectoderm by 70% epiboly.

本文用整体原位杂交的方法揭示了在斑马鱼胚胎发育过程中，Fez基因的表达最早出现在原肠期胚胎头部未来的神经外胚层细胞中，此时胚盘仅下包约70％。

Mutants withdefects in epiboly, axis, somite, head, and cardiac and blood systems were identifiedby observing morphological changes in F3 embryos. Additionally, in situhybridization was adopted to identify mutant lines with altered expression patterns ofdpr2, frb35 and insulin.

通过对 F3 代胚胎的表型观察，鉴定了在外包、体轴、体节、头部、心血管系统等表现异常的突变体；通过原位杂交的方法，筛选了 dpr2，frb35 和胰岛素基因表达异常的突变体。

A genechip analysis was performed using RNAs derived from embryos injected with squint mRNA, MZoep mutant embryos that are deficient in Nodal signaling, and wildtype embryos at the 30% epiboly stage Transcriptswith at least two-fold changes in expression level between wildtype and the other samples were identifyied In squint mRNA-injected embryos, 265 transcripts show anincreased expression level and 111 have a decreased expression level; in MZoep embryos, the expression of 1 495 transcripts increases while 550 transcripts express at a decreased level.

为鉴定受Nodal信号调控的基因，特别是那些转录因子基因，通过将来自squint过量表达、缺失Nodal信号的MZoep突变体或野生型30%外包期胚胎的RNA与Affymetrix斑马鱼寡核苷酸芯片杂交。

Finally,the information about QTLs and epistasis in the population provide strategiesfor manipulation of heterosis and improvement in hybrid rice.

最后，本文就上位性效应对杂种优势遗传研究和杂交水稻遗传改良的影响进行了讨论。

Special equidistance crossover, superior individual reservation, family competition and deterministic selection ensure its ability to search for global optima with high efficiency and avoid premature convergence of GAs.

独特的等距杂交概念，以及良种保留、家庭竞争和确定性选择等策略既保证了其全局寻优的能力，避免了遗传算法的早熟收敛现象，又具有很高的搜索效率。

Our experiment is unique to clone porcine IL-4 gene from lymphocytes of the local species of Chenghua porcine and systematically study its expressing effect in vivo on the immune system and immune responses of mouse and pig to trivalent vaccines against Swine fever, the Pasteurellosis and Erysipelas suis and pig paratyphoid vaccine.

将得到的序列在GenBank和EMBL数据库中进行了同源比较，结果表明它与来源于长白猪肠粘膜固有层淋巴细胞的白细胞介素-4有99％同源，与来源于杂交猪T细胞的白介素-4有96％同源，说明猪种及组织细胞来源的不同可能使基因有一定的差异。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！