杂交

Methods The cDNA retro-transcribed from equal quantity total RNA derived from rectal carcinoma or normal rectal mucosa were labeled with Cy5 or Cy3 fluorescence as probes respectively. The mixed probes were hybridized with the BioStar 4096 cDNA microarray.

分别抽提直肠腺癌和正常直肠粘膜的总RNA；通过逆转录方法将Cy3和Cy5两种荧光分别标记到两组cDNA上，制成探针；利用这种cDNA探针与含有4096条人类基因PCR产物的表达谱芯片进行杂交，扫描芯片荧光信号图像，计算机分析后比较两种组织中差异表达的基因。

The article is opposite our country 2004-2006 year crop of sturgeon of each province city, breed phyletic, seedling to plant on the base that the newest change of the market and field of commodity fish market has descriptive, breed form of water area lack, consumption effectively what in putting forward current sturgeon aquaculture, exist earnestly of guild of disease of control of lack of onefold, crossbreeding, fish and piscine medicine, sturgeon needs to build wait for a problem, offerred the way to deal with a situation in the light of the problem.

本文在对我国2004-2006年各省市鲟鱼产量、养殖种类、苗种市场和商品鱼市场的最新变化进行描述的基础上，提出了当前鲟鱼养殖业中存在的有效养殖水域缺乏、消费形式单一、杂交育种缺乏控制、鱼病和鱼药、鲟鱼行业协会亟需建立等新问题，并针对新问题提出了策略。

Two signals of 45S rDNA were mapped on the mitotic metaphase chromosomes in the F1 hybrids, respectively, and one signal was present on a bivalent during meiotic of synaptene, diakinesis, and metaphase I. We inferred that the two mitotic chromosomes carrying 45S rDNA were homolo-gous pairs.

在有丝分裂中期染色体上2个杂种分别检测到2个杂交信号，在减数分裂粗线期、终变期、中期Ⅰ染色体上45S rDNA位于一个二价体上，说明这两个杂种携带45S rDNA的染色体为同源染色体。

Crosses were made between female parents, four self-selective strains and three common self-crossing strains, and male parents,Mo17 and other 3 main strains, using augmented NCⅡ de sign, to study the inheritance of ear diametre of maize.

以4个自选系和3个常用自交系为母本，Mo17等4个骨干系为父本做7×4格子方杂交，采用增广NCⅡ设计，对玉米穗粗的遗传进行了研究。

The new method combined the use of two DNA probes whose sequences comprises the whole complementary strand to the target DNA, in which one probe was labeled with an EDTA-〓 complex on the 5'-terminus and the other, labeled with a bidentate β-diketone on the 3'-terminus.

该法测定目标DNA的最低检测下限为6 pM，比现有其它均相DNA杂交分析法低2-3个数量级。该法具有简单、快速和高灵敏度等优点，通过使用各种的标记DNA探针，预计该法可广泛应用于各种实际DNA样品的分析。

A second domain, the K-box involved in dimerization, which is conserved among the MADS-box proteins, is also found.

Southern杂交分析标明，BAG基因在苦瓜基因组中有2或3个拷贝，属于低拷贝数的基因。

It was assisted in the process by the environment of the Islands, which produced diminutiveness in all its stock, and by crosses with other breeds residing in, if not indigenous to, the Islands.

而这种小型化正是在岛屿环境下所致的，这种岛屿环境使得上面的一切都变得小小的（怪不得印尼群岛那块的人都小小的呢，哈哈），与此同时和岛上的其他品种杂交所致。

The mongrel wagged its tail enthusiastically when it heard the dinner bell .

那杂交狗一听见吃饭的铃声便起劲地摇着尾巴。

Methods The DIO and DIO R rat models was established by high fat diet. The rats were killed in 2 and 12 weeks. Situ hybridization and immunohistochemistry were used to measure the expression of NPY mRNA and protein in the rats.

高脂饮食建立DIO与DIO-R大鼠模型，分别于2周、12周末处死，采用原位杂交和免疫组化方法检测下丘脑弓状核神经肽Y的 mRNA和蛋白表达情况。

Methods The DIO and DIO-R rat models was established by high fat diet. The rats were killed in 2 and 12 weeks. Situ hybridization and immunohistochemistry were used to measure the expression of NPY mRNA and protein in the rats.

高脂饮食建立DIO与DIO-R大鼠模型，分别于2周、12周末处死，采用原位杂交和免疫组化为法检测下丘脑弓状核神经肽Y的mRNA和蛋白表达情况。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！