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杂交

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This cultivar was derived from the same cross as Himrod.

该品种是从相同的Himrod的杂交后代。

In the experiment, we wish find the methods on shortened cuttage rooting time of hybrid pine.

caribaea扦插繁殖试验,研究缩短杂交松生根时间的方法。

In this research, the ~ and F2 of the crosses between a natural mutant 慪34?with super-minute grain and 慪38?with super-large grain,慡huhui 881擲huhui 527?with middle grain size were carried out to study the inheritance of the grain shape (grain length, grain width, grain thickness and grain length/width ratio) and 1000-grain weight. The main results are summarized as below:? The F1 grain length, grain width, grain thickness and 1000-grain weight of three crosses were lay between the two parents and tended to Y34, which indicated that those grain traits were all governed by the dominant effect of Y34 and influenced by both female and male parents. The differences of F1 grain length, grain width, grain thickness and 1000-grain weight of positive and negative crosses between Y38 and Y34 indicated the existence of cytogene effects.? The broad heritabilities of major grain traits were calculated.

本研究利用一份水稻极小粒自然突变材料Y34与一份水稻极大粒材料Y38、两份常规籽粒大小材料蜀恢881、蜀恢527的杂交F_1及F_2,对主要粒形性状(粒长、粒宽、粒厚、长宽比)及千粒重进行了遗传研究,根据遗传研究的结果利用微卫星标记结合F_2群分法对控制Y34短粒性状基因进行了分子标记定位,主要结果如下:●各组合F_1粒长、粒宽、粒厚和千粒重介于双亲之间且明显偏向于小值亲本 Y34,这表明粒长、粒宽、粒厚和千粒重均主要受小值亲本显性基因的控制并同时也受大值亲本核基因的影响。Y34与Y38正反交F_1在粒长、粒宽、粒厚、千粒重等性状上存在差异,表明存在细胞质效应。

The results indicated: 1) the rate of the PCR positive test for 1 120 transgene carps reached 60%; 2) the rate of the PCR positive test for 258 transgene carps' cross F1 generation reached 53.8%; 3) the rate of the PCR positive test for 36 transgene Carps' cytogenesis F

检测结果为:对1120尾转基因草鱼的PCR阳性检测,检出率为60%;258尾转基因草鱼杂交F1,阳性检测率为53.8%;36尾转基因草鱼雌核发育F1,阳性率为91.7%,说明Hu-IFN-α基因成功导入草鱼中,并能遗传给F1。

He defines the local population or deme as the group of potentially interbreeding individuals at a given locality.

他说地区的种群或居群是某一地区具有潜在杂交能力的个体的集群。

Results After articular cartilage injury, the degeneration and denature of the cartilage occurred, and the cluster expressed collagen type Ⅹ and apoptosis appeared.Conclusion Apoptosis may play a key role in the pathogenesis and prognosis of osteoarthritis.

为进一步探讨骨关节病的发病机制,我们收集了骨关节病的临床标本,采用原位杂交及原位末端标记的方法,观察Ⅹ型胶原和凋亡细胞在骨关节病关节软骨组织中的分布,并探讨凋亡与骨关节病的关系。

In this study, 259 F(subscript 2:3) lines, developed from a cross between a dent corn inbred Dan232 and a popcorn inbred N04, were grown in a replicated experiment and evaluated for three popping characteristics. Of 613 simple sequence repeat primer pairs screened, 183 pairs were selected to construct a genetic linkage map 1762.2cM long with an average interval of 9.63cM.

本研究以普通玉米自交系丹232和爆裂玉米自交系N04杂交构建的259个F(下标 2:3)家系为定位群体,采用完全随机区组设计在郑州夏播条件下测定了3个膨爆特性指标。

Generally, B in the configuration closest cell's DNA conformation, it and the double helix model are similar. A-DNA and when RNA member double helix area as well as duplication forms the DNA-RNA hybrid molecule conformation is close. Z-DNA take the nucleotide dimer as the unit left-hand winding, its principal chain assumes the denticle the shape, therefore.

一般认为,B构型最接近细胞中的DNA构象,它与双螺旋模型非常相似。A-DNA与RNA分子中的双螺旋区以及转录时形成的DNA-RNA杂交分子构象接近。Z-DNA以核苷酸二聚体为单元左向缠绕,其主链呈锯齿形,故名。

Methods A total RNA from ten cases of ovarian cancer and contral normal ovarian tissues were extracted by a single step method. The cDNA retro-transcribed from equal quantity mRNA derived from ten cases of ovarian carcinoma and normal ovarian tissues, which were labeled cDNA strand with Cy5 and Cy3 fluorescence as probes.

按一步法分别抽提10例人卵巢癌组织和对照正常卵巢组织的总RNA并纯化mRNA;分别从10例取等量的人卵巢癌组织和对照组织的mRNA分别混合后逆转录合成以Cy5和Cy3标记的cDNA一链做探针,混合后在含有8464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交

Methods] A total RNA from 10 cases withovarian papillary serous cystadenocarcinoma and normal ovarian tissues were extracted by a single step method of gene chip. The cDNA retro?鄄transcribed from equal quantity mRNA derived from 10 cases with ovarian papillary serous cystadenocarcinoma and normal ovarian tissues. The cDNA strand was labeled with fluorescent Cy5 and Cy3(normal ovarian epithelial cells) as probes.

方法] 采用基因芯片技术,按一步法分别抽提10例卵巢浆液性乳头状囊腺癌患者的癌组织和正常卵巢组织的总RNA并纯化mRNA;逆转录合成以Cy5荧光标记卵巢癌细胞和Cy3荧光标记正常卵巢上皮细胞的cDNA作为探针,在含有8 464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交

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