杂交

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

The sex separate progeny from the cross between Japanese gynoecious and Chinese cucmber were employed to study the molecular markers linked to the sex determination genes of cucumber with RAPD technology.

以日本全雌性黄瓜与中国华南型弱雌黄瓜杂交后多代自交的分离后代，利用RAPD技术对黄瓜性别特异基因进行分子标记连锁研究。

Kite your Cultist out of battle and out of harms way.

利用放风筝的方法让你的杂交信徒离开战场和危险的地方。

Once you make your grab, there is no point in keeping the Cultist near ground zero.

一旦你成功实施控制，没有任何理由让杂交信徒们继续留在危险地区。

It must be noted that the Cultist cannot mind control Epic Units, but any other Tier 3 unit is fair game.

必须指出的是杂交信徒不能控制史诗单位，但是可以控制其他任何三级单位。

That said, once you make your capture, you should kite the Cultist away from the battle and let your new unit do the talking.

这就是说，一旦你成功控制敌人，你应该让杂交信徒离开战区，用放风筝的方式让你控制的新单位来战斗。

If you had to capture say a Rifle Squad to save your Cultist, you can free up your Cultist to mind control something of more value by force firing at another unit.

如果你不得不控制一队机枪兵来救你的杂交信徒，在安全后你可以通过强行攻击其他单位的方式让它们自由的控制其他更有价值的单位。

The Cultist isn't a very fast unit, and is vulnerable to anti-infantry attacks.

杂交信徒速度不快，而且对抗反步兵攻击时非常脆。

Even in this state the Reckoner is still considered a vehicle, so you must watch out for things such as Cultist/Prodigy/Mastermind mind control and larger vehicles running over it.

即使在这个状态，清算者还是被当作车辆来处理的，因此你要小心诸如杂交部队（译者。。不知道中文版里面怎么翻译的。。）/夺魂师/摄魂师的心灵控制部队以及可以碾压它的大型车辆。

Even in this state the Reckoner is still considered a vehicle , so you must watch out for things such as Cultist Prodigy Mastermind mind control and larger vehicles running over it .

即使在这个状态，清算者还是被当作车辆来处理的，因此你要小心诸如杂交部队译者。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！