杂交

A contrastive experiment was conducted on the growth of hybrid sturgeon, namely wild Acipenser schrencki, Acipenser baeri and Acipenser schrencki, Acipenser baeri fed with artificial diets under still water conditions.

在静水条件下，对两类杂交鲟-W类（野生施氏鲟与野生西伯利亚鲟的杂交品种）和D类（人工驯养的施氏鲟与野生西伯利亚鲟的杂交品种）的幼鲟进行了生长对比实验。

To identify whether our previous founding were exist in the series of our newly established cell cultures with the same genetic background and different metastatic potentials, and to make clear what are congenerous and various traits in the chromosomal or DMA sequence levels between these cells, in this study, a combination of conventional G-banding, comparative genomic hybridization, multiplex fluorescence in situ hybridization and arm or locus-specific fluorescence in situ hybridization were used to comprehensively characterize molecular cytogenetics aberrations of the above cell cultures.

为查明我所新建立的这些具有同一遗传背景而又在肝癌的转移潜能上存在明显差异的细胞是否也具有8p的缺失，并弄清它们在其它染色体及其区段的DNA水平上有着怎样的相似和不同，这些相似与不同又有着怎样的意义，本课题联合应用G显带技术、比较基因组杂交技术、多重荧光原位杂交技术和位点或臂特异荧光原位杂交技术，对上述细胞进行细胞及分子遗传学研究，以期了解其分子细胞遗传学异常的特征、找出转移相关的遗传学标记，为探讨肝癌转移机制提供材料。

Chicken and quail intergeneric hybridization is a classic distant hybridization in fowl. In fowl science, chicken and quail belong to Aves, Galliformes, Phasiamidae, Gallus and Coturnix, and this hybridization combination has become the example of researching the incompatibility of different germ cell and sterile of distant hybridization.

鸡与鹌鹑杂交是属间杂交，其杂交种胚胎早期出现大量的死亡，并且杂交种出壳以前雌性胚胎全部死亡，这使鸡与鹌鹑杂交成为研究异种生殖细胞不亲和性和杂交种不育的典型。

In a half diallel cross including 4 parents and 6 hybrids, Bt had significant effect on hybrids lint yield compared with those without Bt insertion.

对一套4×3半双列杂交组合以及2对近等基因系组合的遗传分析和抗虫性鉴定发现，杂交组合在没有Bt基因存在的情况下，Bt基因的引入对于杂交组合的抗虫性增强和产量增加有显著作用。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.739 and 816 PCR positive clones were respectively selected to perform dot blot,and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。对原肠期739个和尾芽期816个PCR阳性克隆进行斑点杂交，得到72个原肠期和98个尾芽期斑点杂交阳性克隆。

In the GISH, a given genomic DNA was hybridized to chromosomes of their own or other species and signals of repetitive DNA sequences were exclusively detected.

一种新的通过基因组原位杂交进行的分子显带方法，即利用一个物种的基因组DNA作探针，对该物种或其它物种的染色体进行原位杂交，其杂交信号的检出程序是专门用于检出重复DNA顺序的。

It was shown that the resultant cybrid HL-R cells incorporated with reticulocyte cytoplasts became differentiated 〓 bytalterationcin growth parameters, which might be defined as decancerization:(1) loss of growth ability in soft agar medium;(2) nontumorigenic under heterotransplantation in their filial cellular generation to mude mice;(3) decrease of growth rate, cellular mitoxic index and DNA synthesis rate;(4) decrease or inhibition of c-myc gene expression;(5) expression of human globin gene products in 〓 subculture.

利用C-myc癌基因探针的分子杂交技术，未检测到胞质杂交细胞存在相当于C-myc基因特异的同源序列mRNA，提示杂交细胞癌基因表达严重受抑。

Methods Femoral arteries isolated from the rabbits which had been fed with a high cholesterol diet and locally perfused with MM-LDL within the artery beforehand, were used as the models. Antisense MCP-1cDNA was transferred into the arterial wall by injecting recombinant LNCX-anti-MCP-1/liposomal complex in the femoral sheath and the periarterial tissue.

以实验性高脂血及轻度氧化LDL局部保留灌注的家兔股动脉为模型，采用DOTAP阳离子脂质体包裹LNCX-anti-MCP-1质粒，将反义MCP-1 cDNA定位导入家兔股动脉鞘及周围组织，以原位杂交、 Northern杂交及狭缝杂交法观察反义MCP-1基因的表达，应用扫描电镜观察单核细胞在动脉内膜粘附的情况。

After purification, PCR products of 768 clones were printed on silanized glass slides automatically using arraying system.

该方法通过将连有不同接头的两种待检样品cDNA分别与过量的对照样品cDNA进行杂交后，合并继续进行第二轮杂交，并且在杂交后利用对应于接头的特异性引物进行两轮抑制性PCR，扩增的产物通常便是同对照样品相比待检样品中差异表达的基因。

Methods Hybridoma cell line excreting monoclonal antibody against total aflatoxins was produced by using B cell hybridoma technique.

方法利用B细胞杂交瘤技术，建立分泌抗总黄曲霉毒素单克隆抗体杂交瘤细胞株。结果建立了1株稳定分泌抗总黄曲霉毒素抗体的杂交瘤细胞株，命名为2G2。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。