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杂交

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By Faradic impedance technique,a significant increase of the interracial electronic transfer resist- ance R_ was determined when the SH-ssDNA probe/Au electrode hybridized with its comple- mentary sequence.

实验中对DNA探针的自组装时间、杂交温度、杂交时间和阻抗测量液等实验条件进行了观察和优化;通过选择自组装液中SH-ssDNA探针和RSH的浓度,减少DNA在金电极表面的非特异性吸附,同时保证金电极表面自组装的SH-ssDNA探针有合适的疏密度,提高了杂交效率。

To breed with another kind or species hybridize .

品种间杂交一另一类或另一物种杂交杂交

Lam. and its related species are called plants of Ipomoea section batatas, which are classified into two groups based on the cross-compatibility with sweetpotato: Group I cross-compatible with sweetpotato and Group II cross-incompatible with sweetpotato.

根据同甘薯的杂交亲和性,将甘薯组植物分为两个群:同甘薯杂交亲和的第Ⅰ群和同甘薯杂交不亲和的第Ⅱ群。

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本,经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后,每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1:GPA-FITC单抗分选男胎脐血NRBCs;2:2号标本;3:CD36-FITC单抗分选男胎脐血NRBCs;4、5:1、3号标本;6:50个男性细胞标本;7:5个男性细胞标本;8:200ng男性DNA;9:未孕女性DNA;10:ΦX174 HaeⅢ标准,271bp:为SRY基因扩增带;383bp:为人β-珠蛋白基因扩增带内参照

The breeding scale of hybrid snakehead (Channa argus♂×Channa maculate♀)waslarger and larger in south of China. Three important factors witch lead to low survivalrate was studied, including prey density, stocking density and cannibalism.

杂交鳢是乌鳢父本和斑鳢母本,通过杂交获得的F_1代,在苗种培育时期易发生残食现象,常使存活率大大降低,严重制约了杂交鳢大规模养殖和推广。

FISH with the tomato 45S rDNA clone revealed very strong signal in the satellite on the short arm of chromosome 2 as well as weak signals in five CPD banded regions at pachytene or four pairs of CPD banded regions at metaphase.

用番茄的一个45S rDNA 克隆进行的荧光原位杂交,不仅在位于2号染色体短臂的随体上显示了强的杂交信号,而且在粗线期染色体的5个CPD带区或有丝分裂中期染色体的4对CPD带区显示了弱的杂交信号。

The fact that the pink plants were predominant among the population of RP1 cross progeny hints that the pink had the strong descendiblity, Whereas another fact that the plants with the color of another parent were predominant among the population of R01 cross progeny indicates that the nacarat/saffron plant had the low descendiblity. The above results suggest that it is better to choose the pink plants with the target color flower of breeding as a parents when arranging the cross combination. Many novel flowers with singular value of view and admire can be bred by the different cross combination since the wide segregation chromatogram is not confined to the parents'.

以RPl及R01作母本或父本,分别与不同花色杂交,F1代发生了明显的花色分离,RP1的杂交后代均以粉色占优势,表明粉色的遗传力较强,R01的杂交后代均以其它亲本占优势,表明桔黄/桔红的遗传力较弱,因此在选配组合时,最好选择具有育种目标的粉色个体作为亲本;另外由于分离谱较广,且分离的色谱范围并不完全限于双亲之间,可根据不同的杂交配组,培育出具极高观赏价值的稀有花色。

In chapter 4,on the basis of chapter 3,a new formulation of hybrid/mixedsingular plate element based on Reissner's plate theory is set up,and 4-node arbitraryquadrilateral hybrid/mixed singular bending plate element formulas including theeffects of transverse shear deformation is derived,then by the use of this method,alarge quantity of numerical computations are carried out,at the same time,the resultsare compared with theoretical solutions and the results obtained by other singularfinite element methods.

第四章 在第三章的基础之上,建立基于Reissner型板理论之上的杂交/混合奇异板单元列式,推导出了考虑剪切效应的四节点任意四边形杂交/混合奇异弯曲板单元公式,并进行了大量的数值计算,同时与理论解析解和其它有限元解进行了比较,计算结果表明:本文提出的杂交/混合奇异板单元方法是可以用于计算含裂纹平板弯曲应力强度因子的一种高效、可靠、简便易行的方法。

And it is especially applicable to quick measurement for great batch of samples.3 MT dynamics in blood , feces and urine,and MT accumulation in tissues and organs of DLY pigs and WD pigs were also conducted by a isotopic tracing method using MT labeled with ~(125)I ~(125)I-MT which was affused through mouth..It was shown that MT was absorbted perfectly with a tardigrade mode and remained about 31 hours in the digestive tract of both DLY pigs and WD pigs . MT excreted from feces and urine of DLY pigs accounted for 7.91 and 21.45 percent of total MT affused through mouth while MT excreted from feces and urine of WD pigs accounted for 10.09 and 11.85 percent of total MT affused through mouth, respectively.

灵敏度为4.1ng/ml,批内变异系数和批间变异系数分别为9.06%和12.35%,具有特异性强、灵敏度较高、稳定性和重复性好、适合于大批量样品检测等特点。3利用~(125)I标记MT~(125I-MT进行同位素示踪,研究了MT在杜长大杂交猪和杂种野猪体内吸收速率、代谢过程、排泄途径、在各组织器官中存留规律和代谢动力学,发现MT在杂交猪和杂种野猪体内能被完整地吸收,但吸收速度较慢;MT在消化道内存留时间为31h左右;MT在杜长大杂交猪粪中和尿中排出量分别占灌喂总量的7.91%和21.45%,杂种野猪相应值分别为10.09%和11.85%,二者尿中排出的MT量差异显著(P.01)。

These results suggested that the newly hatched hybrid fries between Pseudosciaena Crocea ♀ and Nibea albiflora ♂ contained the genome of both parents, and the phenomenon of uniparental gene elimination was not obvious.

这些结果表明,大黄鱼♀×黄姑鱼♂杂交F1初孵仔鱼中同时含有来自双亲的基因;虽然计算结果显示杂交F1在遗传上略偏向于母本,但是父源基因和母源基因没有明显的选择性丢失;母本特异位点多态比例与大黄鱼种内杂交F1相当。

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If you have any questions, you can contact me anytime.

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