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杂交

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In this study, AFLP markers for YrSp gene were identified and SCAR markers were developed using the near-isogenic line YrSp/6 x Avocet S and its susceptible parent Avocet S as materials with the YrSp gene donor parent Spaldings Prolific as control. The main results were given as follows:1. A total of 225 primer combinations were used in AFLP analysis of the materials YrSp/6×Avocet S, Avocet S and Spaldings Prolific.

本研究采用AFLP分析方法,以小麦抗条锈病基因YrSp的供体亲本Spaldings Prolific、近等基因系YrSp/6 x Avocet S、轮回亲本Avocet S及由Avocet S和YrSp/6 x Avocet S杂交制备的F_2代分离群体为材料,开展了小麦抗条锈病基因YrSp的分子标记研究,取得了如下主要结果: 1。

aim: to explore the alteration of eta 1 expression in motoneurons following spinal root avulsion and its significance.

首先建立脊神经根性撕脱伤的动物模型,采用原位杂交和免疫组织化学方法检测损伤前后eta 1 mrna在脊髓运动神经元中表达的变化。

Backcross A cross between an individual and an individual of the original parent generation.

回交:杂交产生的子一代个体再与其亲本进行交配的方式。

Its utilization potential is more powerful in the breeding of recurrent-selection, cross, backcross and multiple-cross.

探讨了单显性核不育的利用方法和途径,单基因显性核不育在轮回选择、杂交、回交和复交育种中有一定的利用价值。

The variance component of cross and backcross generations of quantitative character controlled by multigenes is studied on condition of chromosome translocation .

研究了染色体易位情况下,多基因控制的数量性状杂交及回交世代的方差组成。

By analyzing the DNA of excellent soybean strains and their parents,it is made clear that backcross should be carried out in early generation so as to retain more genetic basis of wild soybean.

通过对大豆优良品系及其亲本的DNA组成分析,明确了用栽培大豆与种间杂交后代回交应在早代进行,以便更多地保留野生大豆细胞核的遗传基础。

Its alterations of phenotype and structure have been further studied at whole, organic and cellular levels. By means of backcross between wildtype and the mutant, its genetic background has been analyzed.

本文在整体水平、组织水平和细胞水平上深入研究了该突变体的表型和结构的变化、通过突变体与野生型之间的杂交对其遗传背景进行了分析,并运用分子生物学手段对突变体相关基因进行了克隆和定位,初步探讨了该基因的表达模式和功能。

The maps are not,however,desirable and entire because all of the markers on the maps come from the no recurrent parent.The consequence is that number of the markers used for map in backcross population is never more than half in intercross population.

但是,用回交群体作图所得的图谱是不完整的,因为图谱上所有的标记都是非轮回亲本提供的,因此标记数不会超过杂交F2群体的一半。

After several generations you will need to backcross to one of the previous strains.

必须继续保留原线鱼种,因为经过异种杂交后往往需要原线鱼种做反交。

Methods:The expression of bak gene mRNA in 52 cases of bladder transitional cell carcinoma was detected by in situ molecular hybridization.

应用原位分子杂交方法检测52例膀胱移行细胞癌组织中的bak mRNA的分布与表达。

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