杂交

The result of ISH indicate that the FIE gene has specialspatio-temporal expression in apomixis E.binata during the reproduction growth andcontrols the development of embryo and endosperm.

原位杂交的结果表明FIE在龙须草生殖发育的过程中特异的时空表达，与雌雄配子体发育有关，并参与调节胚和胚乳的发育。

SSR analysis indicated that triploids were derived from nucellar apomixis; diploids were most probably from sexual crosses of haploid female gametes and alien pollen grains.

SSR分析结果表明三倍体后代来源於柑橘珠心细胞的无融合生殖，而二倍体个体很可能是单倍体的雌配子与外界花粉杂交后代。

Apomixis allows the establishment of genetically stable seed propagating clones of crops,which can perpetuate themselves across countless sporophytic generations.

利用植物无融合生殖固定杂种优势，已被认为是一条生产杂交种子的高效途径。近年来，由于RAPD、RFLP和差异显示等技术的应用，已使植物无融合生殖的研究面貌一新。

We also draw the conclusions from the review as follows:①RAPD, SSR and AFLP can be used to analysis the variation of peanut;②The deep generations of hybridized peanut can be identified by RAPD ;③Use RFLP and RAPD to draw the fingerprinting of peanut;④The research on Bradyrhizobiumsp Arachis;⑤The progress in the technique of RFLP and RAPD based on peanut research.

主要介绍了RFLP、RAPD、SSR、AFLP等四种分子标记技术的原理、方法、特点及其在花生上的应用现状：①RAPD、SSR、AFLP都可以分析花生品种的多样性；②RAPD技术可以对杂交后代进行鉴定；③利用RFLP、RAPD技术绘制花生指纹图谱；④利用分子标记技术研究花生根瘤菌；⑤基于花生的RFLP、RAPD技术的改进。

Euler early after the sheep and Tibetan wild argali sheep constantly cross-breeding of the skin after the formation of a combination of high-quality goat type species, with strong disease resistance, cold resistance, fitness, and growth and development of fast, high-volume production of meat, fresh meat No smell of mutton taste, commodity rates, operation agility, teamwork and strong resistance, feeding coarse, easily bred and hides, and skin thickness, strong, flexible, and strong features such as heat, is the best skin products market.

欧拉羊是早期经过野生盘羊和藏系羊不断杂交，繁育后形成的皮肉兼用型优质羊种，具有抗病力强，耐高寒，体格大，生长发育快、产肉量高、肉质鲜嫩无膻味，商品率高，行动敏捷，合群性强，耐粗饲、易饲养及皮张大，皮板厚，结实、柔韧性好、保暖性强等特点，是皮肉市场中的上乘品。2005年在首届青海高原特色农畜产品展示会上被评为优质畜种。

Atlas Human cDNA expression array technique was employed to study the differences in gene expression in normal and endometrial cancer tissues.

为了鉴定正常子宫内膜与子宫内膜癌组织之间差异基因的表达，采用AtlasTM 人表达阵列技术，首先从正常子宫内膜和子宫内膜癌组织获得以α 3 2 P标记dATP的cDNA ，然后与两个相同的AtlasTM人表达阵列膜(含 5 88个已知基因)杂交。

The differential expression profiles of 588 known genes were determined using Atlas human cDNA expression array. Fifteen genes were differentially expressed following the overexpression of BNIPL-1. Most of them were involved in cell proliferation or cell apoptosis. Furthermore, the differential expression result was confirmed by semiquantitative RT-PCR.

然后采用人Atlas cDNA expression array对新基因BNIPL-1在细胞内的下游调控事件进行了初步研究。588个已知基因中有15个的表达发生了改变，其中大部分是细胞生长和凋亡相关基因，半定量RT-PCR验证了杂交结果的可靠性。

The aim of this study was to characterize the expression of BMPs in synovial tissues under normal and arthritic conditions.

对来自正常人和骨性关节炎类及风湿性关节炎患者的滑液组织分析骨形态发生蛋白的表达，利用微点杂交。

Sixty single ascospore strains of Morchella angusticeps Boudier were crossed between each two single ascospore strains.

本文对自黑脉羊肚菌Morchella angusticepsBoudier分离到的60个单孢菌株进行了两两杂交实验。

A cross was made between two isolates of Magnaporthe grisea, CH63 and TH16. A total of 78 ascospore isolates were tested for pathogenicity on 36 rice varieties. The 71 pathotypes were clearly observed in the sexual progeny.

报导了两个稻瘟菌株CH63和TH16的杂交后代在36个水稻品种上的致病性分离、遗传分析以及无毒基因组成的分析结果。78个子囊孢子菌株中呈现显著的致病性分离，共出现71种致病类型，在不同品种上分别出现1：1、1：3、3：1、1：7、1：15和15：1等的无毒性／毒性分离，表明供试菌株对不同品种的无毒性／毒性是一个或多个基因控制的。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！