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杂交

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In this paper, major agronomic traits and grain quality traits affecting rice yield and grain quality were investigated in seven aromatic rice maintainer lines bred in Sichuan and one variety introduced from America. It was aimed at estimating the numbers of gene controlling aroma, the allelism of aroma genes, location of aroma gene on the chromosome, and molecular tagging of aroma gene.

本研究利用四川新育成的七个香稻保持系(D香1B、D香2B、内香2B、内香4B、绵香2B、绵香3B、宜香1B)、一个香稻引进改良品种(泸香90)和一个非香型保持系为材料,研究了香稻保持系主要农艺性状和品质性状对品质、产量的影响,并且通过杂交群体构建对香稻香味性状进行了遗传分析。

The pollination by 3 years has been tested , has affirmed T. chinensis belonging to common allogamy plant, has provided the theory basis to T. chinensis crossbreeding.

通过三年的不同授粉方式试验,确认了金莲花属于常异花授粉植物,试验结果为金莲花的杂交育种提供了理论依据。

The properties of the hybrid, such as greater vigor and adaptability, are retained in the allopolyploid in subsequent generations and such organisms are often highly successful.

这种具有高度生活力和适应性的杂交在异源多倍体中被保留,而且可以成功的遗传下去。

Third,apomictic rice and C4 crop,were crossed to create new allopolyploid cultivars with high photosynthetic efficiency.

无融合生殖水稻和C4作物杂交创造高光效异源多倍体新作物。

Methylation variations of artificial F4 and F10 generations of Raphanus sativus×Brassica alboglabra allopolyploid were examined with MSAP. The results showed that the methylation levels of F4, F10 were 30.61% and 33.10%, respectively.

以人工合成的萝卜-芥蓝异源四倍体的F4和F10世代为材料,用MSAP法检测2种材料DNA甲基化变异情况,以期为远缘杂交后不同世代间基因组DNA甲基化遗传和变异规律提供实验依据。

Discussions are given of the fate of Ms2 during translocation in the hexoploid triticale, the exchange of the names for 4A and 4B chromosomes in common wheat and the possible expoitation of the new locus Ms2 (4BS), and the following speculations are made: In geneic genes of allopolyploid organisms the donor chromosomes tend to be intergenomically translocated to their phylogically and evolutionarily close chromosomes with the same order number and the same arm; it is cofirmed that the 7th International Conference of Wheat Genetics was right to exchange the names between chromosomes 4A and 4B of common wheat in 1988; and as a new genetic marker and a breeding tool for all the chromosome B-carrying species in the tribe of Tritceae, Ms2 (4BS) may have wide application in building and expanding the gene pool of germplasm resources of various species of wheat.

对 Ms2基因在六倍体小黑麦与原太谷核不育小麦远缘杂交中易位时的走向,普通小麦4A与4B染色体的互换更名以及 Ms2(4BS)新位点的开发利用进行了讨论:认为异源多倍体生物核基因的组间易位倾向于从供体染色体向进化亲缘关系较密切,且染色体序数与染色体臂相同的部分同源染色体易位;1988年第7届国际小麦遗传学会对普通小麦4A与4B染色体的互换更名是正确的; Ms2(4BS)作为一个新型的遗传标记,作为小麦族内所有携带B染色体组的物种的育种工具和在拓建各类小麦种质资源的基因库等方面均有广泛的用途。

This study was to explore the relationship of genomes between Oryza alta and Oryza latifolia using genomic in situ hybridization.

[目的]采用基因组原位杂交技术研究稻属2种CCDD基因组型的野生稻宽叶野生稻和高杆野生稻基因组之间的关系。

Results: The expression of both mRNA and protein of c-myc were obvious in the bud and cap stages,while decreased in the bell stage, then increased in ameloblast and dentinoblast when dentin formation.

利用原位杂交技术、免疫组织化学技术检测SD大鼠牙胚发育不同时期c-myc mRNA转录及蛋白的表达情况。

The products were identified by hybridization with its specific oligonucleotide probes labeled with amidogen in a Nylon membrane.

构建二重PCR扩增NGDNA,然后与固定在尼龙膜上的特异性寡核苷核探针杂交

Gonorrhoeae was amplified with the one set of primer for the 16S rRNA gene. The reverse primers were labeled with biotin. The products were identified by hybridization with its specific oligonucleotide probes labeled with amidogen in a nylon membrane.

选择NG 16S rRNA基因设计一对PCR引物,生物素标记下游引物扩增NG DNA,然后与固定在尼龙膜上的特异性寡核苷核探针杂交

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