杂交

The SPAG8 gene was a new gene and assigned the accession number U12978 by GeneBank.

原位杂交实验显示SPAG8基因位于人类第9号染色体的短臂上。

A new expressed sequence tag (GenBank accession number: BI740300) matched completely with human MOB gene was obtained.

从胎儿和成人肾脏消减杂交cDNA文库中获得了一条新的表达序列标签(expressed sequence tag，EST)序列，该序列对应于功能未知的MOB基因。

The results observed showed that pathological changes consisting of destructive meningoencephalitis and large, basophilic, intranuclear and acidophilous intracy toplasmic neuronal inclusions of viral type occurred in the fetal mouse brain.

待雌鼠临产时剖腹取出胎鼠脑双侧大脑皮层，进行病毒分离、病理学检测及用地高辛标记的HCMV寡核苷酸探针对大脑皮层细胞压印片进行原位分子杂交检测。

The percentage of through the platform position was calculated. The learning and memory behavior was observed mainly in rats of every group. After the experiment, sodium pentobarbital was injected by intraperitoneal injection of all the rats for anesthesia, and the blood was gained from heart.The plasmcortisol and adrenocorticotrophic hormone were detected by the radioimmunoassay.

取模型组大鼠8只，对照组大鼠5只，不经灌注固定，冰台上迅速分离大脑，将大脑沿矢状缝分左右半球，左半球用于糖皮质激素受体mRNA原位杂交，右半球采用薄层扫描法测定谷氨酸和γ-氨基丁酸含量，同时取大鼠左侧肾上腺进行苏木精-伊红染色，观察肾上腺组织形态变化。

At day 3, 6, 9, 14, 28, 42 and 56, five rabbits were selected from every group randomly and were killed by aeroembolism respectively. Their radius were taken out and the left one was taken as the research object. Immunohistochemistry stain and in situ hybridization stain were performed to examinate the VEGF and VEGF mRNA expression in the haematoma, fibrous callus and soft callus.

分别于术后3，6，9，14，28，42，56 d每组随机处死5只家兔，取左侧桡骨标本作为观察对象，通过免疫组化和原位杂交方法检查骨折端血肿、纤维骨痂和软骨骨痂中VEGF及VEGF mRNA表达。

Objective To study the advantages of dual-color fluorescence in situ hybridizationin sex chromosome analysis of gonad agenesis patients over traditional chromosome analysis.

目的 探讨荧光原位杂交技术比常规染色体分析，在性腺发育不全患者性染色体检测中的优越性。

When C.farreri×P.yessoensiswere tested by using6pairs of microsatellite primers which could be applied to boththe two kinds of scallops,the specific bands could be amplified fromeachone of the 6 pairs,among which,the specific bands fromboth agnate and maternal colony could be distinguished obviouslyinthe amplified products of P13F449 and KMY134,and accordinglythe hybrid identity of hybrid generations could be affirmed.

将这6对具有属间通用性的微卫星引物对栉孔扇贝×虾夷扇贝进行PCR扩增，均能扩增出特异性条带，其中引物P13F449和KMY134的扩增产物中可以明显地分辨出来自父母本群体的特有条带，从而确定了杂交子代的杂种身份。

Yuan Longping is a world-famous agronomist with the reputation of "the Father of Hybrid Rice".

袁隆平是世界著名的农学家，被誉为"杂交水稻之父"。

My many successful outcrossing experiences since Alida underline why it is extremely important not to cut off our own noses to spite our faces.

自从Alida以后我的许多成功的异型杂交的经验强调：为什么不要切断我们的鼻子使之困扰我们的脸是极其重要的。

The Effects of ED -71 on proliferation and differentiation were detected by MTT,.cell cycle, alkaline phosphatase activity and level of osteocalcin.

采用细胞形态学，碱性磷酸酶染色，培养细胞匀浆上清测定单位蛋白下的碱性磷酸酶活性，培养基中骨钙素含量分析及原位杂交方法检测ED-71对OS-732人成骨样细胞分化的影响。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！