杂交

Northern analysis revealed that it was inducible by drought and cold stresses as well as ABA application.

Northern杂交结果表明，该基因的表达受干旱、寒冷及ABA的诱导。

In situ hybridization was used to localize IL-4, IL-6, IL-1β, IL-2, IFN-γ and TNF-α mRNAs in abomasum of the negative control group, challenged control group, vaccinated-challenged group and vaccinated-unchallenged group goats immunized with rHco-GAL-f/m, and the numbers of cytokine mRNA(superscript +) cells in each group were also analyzed.

用rHco-GAL-f/m免疫山羊，通过原位杂交法对阴性对照组、攻虫对照组、免疫攻虫组和免疫不攻虫组山羊皱胃中白介素－1β(IL-1β)、白介素－2(IL-2)、白介素－4(IL-4)、白介素－6(IL-6)、干扰素－γ和肿瘤坏死因子－αmRNA转录进行定位，并对上述细胞因子阳性反应细胞数量进行分析。

The effect of abscisic acid was best among four agents. But it was too expensive to apply to seed production of hybrid rice.

结论］4种药剂中，以脱落酸效果最好，但是其价格昂贵，在杂交水稻制种上无法应用。

Based on the cDNA sequence (GenBank Accession Number: AY515506), 8 pairs of primers were designed using the software 'Primer premier 5.0'.

对上述杂交组合后代群体的遗传分析表明，PPO活性主要受两对主基因控制。

By removed ovary culture after positive and negative hybridization, produced interspecific hybridization, and have improved the hybrid seed through removed ovary culture and induced to shoot by culture medium MS, and produced hybrid F1, the plant of Brassica junce×B.pekinesis, the advantage characteristic of hybrid plant is similar to female nature;By cytology authenticating, chromosome number of F1 young flower bud cell (2n=28), it is the sum of monoploid chromosome number of female parent Brassica junce(2n=36) and male parent B.pekinesis(2n=20); The hybridization to Brassica junce×B.pekinesis, and embryonic bud come from removed ovary culture, and it found another form of plant embryo saving.

以芸薹属植物芥菜型油菜和白菜为研究对象，对其进行常规正反杂交后子房的离体培养，通过离体子房培养获得的杂种种子经MS培养基诱导成苗，获得了芥菜型油菜与白菜的杂种F1代植株，表现出杂种优势；通过细胞学的方法，鉴定了杂种F1幼蕾体的细胞染色体数为2n=28，为母本芥菜型油菜（2n=36）和父本白菜（2n=20）的单倍体染色体数之和；芥菜型油菜与白菜常规杂交后，经离体子房培养获得了&胚性芽&，确立了植物胚挽救的另一种形式。

Indica variety ZYQ8 is an important genetic resource resistant to rice blast disease in rice breeding in North China.in this study,resistance identification of the populations of F1,F2,DH and B1F1of the crosses of ZYQ8×Jingxi17 (JX17) and ZYQ8×Lijiangxintuanheigu was carried out by using a Chinese differential strainZH10-8-14 from North China and a Japanese differential strain Ken54-04 from Japan and the results demonstrated that ZYQ8 had one dominant major gene Pi-zh which had been reported by Zhu L.H., et al., The results from experiments in which the F2 populations of ZYQ8×nine different varieties with known resistant genes were inoculated with strain Ken54-04 indicated that the gene Pi-zh was a new gene and non-allelic to nine known gene loci,namely Pi-i,Pi-km,Pi-z,Pi-ta,Pi-ta2,Pi-zt,Pi-kp,Pi-b and Pi-t.

籼稻品种窄叶青8号是我国北方稻区水稻育种上重要的稻瘟病抗源之一。本文利用我国北方稻区的代表菌系中10-8-14和日本的代表菌系研54-04，对窄叶青8号与感病品种京系17号和丽江新团黑谷的杂交F1、F2、DH和B1F1群体进行抗病性鉴定。根据抗病性的分离，确认窄叶青8号的抗性由1对显性主效基因，即朱立煌等报道的Pi-zh基因控制。利用菌系研54-04接种窄叶青8号与9个具有已知抗病基因的鉴别品种杂交的F2群体，各群体都表现二基因的独立遗传，证明Pi-zh基因与Pi-i、Pi-km|、Pi-z、Pi-ta、Pi-taz、Pi-zt、Pi-kp、Pi-b和Pi-t等9个已知抗病基因间存在非等位关系，是新的抗稻瘟病基因。

In order to study the genetic laws of alkaline water retention capacity in wheat, which might be beneficial for high quality wheat breeding, genetic analysis was conducted in six parents and their F1 obtained from a diallel cross without reciprocals. Six parents characterized with different quality triats in kernel hardness, protein content, gluten strength and AWRC.

为给优质小麦选育提供参考依据，以籽粒硬度、蛋白质含量、面筋强度和碱水保持力不同的6个常用优质小麦品种为亲本，按Griffing双列杂交法Ⅱ配制15个杂交组合，研究了小麦碱水保持力的遗传规律。

The results showed that the effects of nutrition level on average daily gain,feed conversion rate and meat quality had no significant difference;on fatback thickness and ham percent were significant.Composite selection index was used to evaluate the effect of nutrition level on finishing pigs,the results showed that the effect of high nutrition level on crossed finishing pigs was the best during body weight of 35~100 kg. Nutritional level ; Finishing pigs ; Growth performance ; Carcass quality ; Meat quality ; Comprehensive selection index

本试验将日粮消化能、蛋白质和赖氨酸设计高、中、低三个档次，以杜洛克公猪与莱芜猪合成系母猪配套生产的杂交猪为对象，进行了饲养试验和屠宰试验，旨在探讨日粮营养水平对杜莱杂交商品猪的生长性能及肉品质的影响，为养猪生产提供参考。1材料与方法1.1试验猪选择与分组选择出生日龄基本一致、生长发育良好、体重35 kg左右的杜莱断奶仔

Methods: penicillin, as a kind of hapten, was conjugated with carrier protein to form complete antigen and then was used to immunize balb/c mice. hybridoma cells secrecting mab against penicillin were developed.

将半抗原青霉素和载体蛋白偶联后免疫balb/c小鼠，应用杂交瘤技术建立稳定分泌抗青霉素mab的杂交瘤细胞株。

As the ecological conditions of the producing areas to adapt to its development, the mid-90's Gansu Agricultural University Science and Technology Commission and Jingyuan County, the use of Zhongwei goats as a female parent, male parent for hybrid Angora goat hair used to cultivate Gansu-goats, the effect is very clear , The third-generation hybrid produced their own wool and mohair similar.

由于产区的生态条件适应其发展，90年代中期甘肃农业大学与靖远县科委利用中卫山羊作为母本，安哥拉山羊作父本杂交培育甘肃毛用型山羊，其效果十分明显，杂交三代所产毛己与马海毛近似。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！