杂交

For transgenic mice,98%~100% of metaphases and 85%~94% of interphases showed hybridization signal.For negative control mice,100% of metaphases and 95%~96% of interphases showed no hybridization signal.

阳性转基因小鼠98%~100%的中期分裂相，85%~94%的间期核出现杂交信号；阴性对照小鼠100%的中期分裂相、95%~96%的间期核未出现杂交信号。

Brief probe hybridized with long target DNA, the hybridization position had no effects on the binding amount, but mid-position of hybridization would prolong the hybridization time, which was due to the steric hindrance on the surface.

结合位点不同对杂交量无明显影响，但由于晶体表面DNA空间结构的影响，结合位点位于靶序列中间时杂交时间明显延长。

By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用电击法将〓表达载体导入HL-60细胞中，经G418筛选培养20天后再行Southern杂交检测外源〓整合情况，利用RNA狭线杂交法证明了在转录水平上表达有所提高，进一步进行了流式细胞仪测定DNA含量，使细胞在〓期产生生长阻滞作用，细胞生长速度，在软琼脂中成集落数、在组织中的浸润性及在裸鼠中的成瘤性均发生变化，我们的实验结果表明TGF〓对HL-60细胞具有抑制恶性表型的作用。

In order to investigate the genetic variation of smut resistance in cross progenies and to construct the corresponding resistant and susceptible near-isogenic pools, some single lines derived from two cross hybrid combinations (CP84-1198×Ke5, ROC20×Gui73-167) were taken as the plant materials, and inoculation experiment was conducted in two crops of plant cane, and consequent relationship among the 5 indexes of infection was analyzed.

为研究甘蔗杂交后代黑穗病抗性的遗传变异，构建抗病、感病近等基因池，配置了2个杂交组合，其中组合1为CP84-1198×科5，组合2为ROC20×桂糖73-167，对2个组合部分后代进行2次新植人工接种黑穗病抗性鉴定，调查并分析了5个病害参数的关系。

In this paper,the inheritance and selec ti on of fruit firmness of strawberry were studied for 8 F 1 progenies.

通过对草莓 8个杂交组合实生果实的果实硬度分析，探讨了果实硬度在杂交育种中的遗传情况及选择问题。

Yesterday, the national 杂 hand overed the paddy rice engineering technique research the center the outside parties expert, piece, the good news for its 茂 return toing growing sand, he at and at that time, Chinese 杂 hand overing the 稻 at Guinea planting succeeding and realizing yield turn overing the 番 the report to hand overed the paddy rice's father to "杂" the 袁隆 is evened.

昨日，国家杂交水稻工程技术研究中心外派专家张其茂回到长沙，他在第一时间，把中国杂交稻在几内亚种植成功并实现产量翻番的好消息报告给了&杂交水稻之父&袁隆平。

Guinea the 杂 hand overed the paddy rice experiment the 田, witness to hand over the paddy rice's father with the "杂" for the representative's world agriculture scientist to 拳 of people Africa 拳 love heart.

几内亚杂交水稻实验田，见证了以&杂交水稻之父&为代表的世界农业科学家对非洲人民的拳拳爱心。

Hybrid winter-blooming begonia grown for its many large pink flowers; derived from B dregei and B socotrana.

杂交而来的冬季开花的秋海棠，粉色的花大而美丽；由亚洲的一个种和非洲南部的一个种杂交产生。

A bath taken in a bathtub.盆浴 hybrid winter-blooming begonia grown for its many large pink flowers; derived from B dregei and B socotrana.

杂交而来的冬季开花的秋海棠，粉色的花大而美丽；由亚洲的一个种和非洲南部的一个种杂交产生。

Seven hybrid variety were obtained with Temian S1 as parents in 2003,Nongza 310 and 313 were two better varieties.

在2003年，利用特棉S-1为母本配制了7个杂交组合，在各杂交组合的品比试验中以农杂310、313的表现最佳。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！