杂交

Epithelial cells showing hybridization signal in cytoplasms predominated in the positive cells.

杂交阳性细胞中，胞浆出现阳性杂交信号的细胞占主要部份。

With the increasing of Pb and Cd concentration and the prolongation of the time, the net photosynthetic rate, stomatal conductance, transpiration rate of all hybrid tulip trees decreased and the downtrends of NE60 and NEF14 which were stress- resistence were small. But the changes of intercellular CO2 concentration were difference.

在Pb、Cd胁迫下，杂交鹅掌楸净光合速率、气孔导度和蒸腾速率均随胁迫浓度的增加和胁迫时间的延长而下降，且抗性较强的NE60、NEF14降幅较小，而胞间CO2浓度随浓度和时间变化各杂交鹅掌楸变化存在差异。

In this article,we report a study on the duplex stability affected by mismatches and insertions.The effects of the chemical nature,the positions,the numbers,and the cooperative behavior of mismatches as well as insertions on the 20-mer and 30-mer duplexes were examined.

在本实验中，我们系统地研究了20-mer和30-mer的杂交双链中错配与插入碱基的化学性质，位置，数目及协同效应对杂交双链稳定性的影响，总结了相关的稳定性趋势；应用温度梯度PCR实验来验证错配双链的不稳定性。

Forward and reverse subtractive cDNA libraries were constructed using suppression subtractive hybridization method,using 10 DAP and 20 DAP endosperms of the two inbreds.The four subtracted cDNA libraries enriched gene expressed specifically or at higher level in early stage and middle stage of endosperm development for N04 and Dan232,respectively.

利用抑制差减杂交方法，分别构建了自交系N04和丹232 10 DAP和20 DAP胚乳的正反向4个抑制差减杂交库，分别富集了N04胚乳早期、N04胚乳中期、丹232胚乳早期和丹232胚乳中期增强表达的基因。

In eucalypts, for example, Corymbia ficifolia cannot be reliably distinguished from its close relative Corymbia

然而， 很难去预测未来杂交区域的变化；无论把具有杂交的种群定为两个种还是一个种，都具有潜在的争议性。

The Effects of fertilizer on the growth and dry weight of sorghum hybrid grass were studied by pot experiment.

高粱杂交草作为一种新的饲料作物，2 0世纪 80年代以来受到了我国高粱育种家的重视，钱章强等在国内首例育成了高粱杂交草皖草 2号并已审定推广[1～ 3] 。

The property of the hybridoma cell lines to secrete expected antibody was confirmed by ELISA and Western blotting analysis.

杂交瘤细胞产生的抗体可以作为相关实验的材料，杂交瘤细胞也可作为从事植物抗体研究时抗体基因的来源。

Cells inosculation was carried out by standard method.The hybridoma supernatants were selected by indirect ELISA.The selected cells were subcloned three times by limiting dilution .Three hybridoma lines against TGEV rMP were obtained , which were designated as 1B3、3C2、4A5. The isotypes of the McAbs were IgG2a、IgG1、IgG1. The three hybridoma lines can secrete antibodies steadily after culturing and saving for a long time.

并以纯化的M蛋白为抗原，免疫BALB/c小鼠，按常规方法进行融合，间接ELISA方法进行筛选，采用有限稀释法，经过三次克隆，最终获得三株抗重组TGEV M蛋白的杂交瘤细胞系(命名为1B3、3C2、4A5)，分泌的单克隆抗体分别为IgG2a、IgG1、IgG1类，三株杂交瘤细胞在体外长期培养和长期冻存时都稳定地分泌单克隆抗体。

The yield and purity of each DNA sample was tested by UV-spectrophotometer, the integrality of these DNA samples were run electrophoresis on the agarose gel. Each DNA sample was subjected to PCR amplification and hybridization using One lambada rSSO HLA-A,-B and -DRB1 commercial kit, the fluorescent intensity for positive bead and negative bead hybridized with HLA-A,-B and -DRB1 PCR products were calculated and analyzed.

使用2ml深孔板和TECAN DNA全自动工作站，从288份骨髓捐献者样本中提取基因组DNA；提取的DNA样本用紫外分光光度仪测定其浓度和纯度，并采用琼脂糖凝胶电泳检测DNA的完整性；DNA样本用One lambda rSSO HLA-A、B和DRB1基因分型试剂盒进行PCR扩增、分子杂交和Luminex流式磁珠分析仪检测，统计分析每一DNA样本HLA-A、B和DRB1基因扩增产物经DNA探针分子杂交后的阳性磁珠和阴性磁珠的荧光信号强度。

The long specific DNA probes were synthesized by polymerase chain reaction in the highly specific gene sequences of above 10 bacteria. The species-specific probes synthesized were inoculated on a nitrocellulose filter and hybridized with template DNA labeled with long-arm photosensitive biotin. The signal of hybridization reaction was observed to detect which bacteria infected. The sensitivity and specificity of the method were studied.

在这10种病原菌高度特异的基因序列内利用聚合酶链反应合成上述10种细菌特异的长链DNA探针，并将合成的特异探针固定在硝酸纤维素膜上，与用长臂光敏生物素标记后的模板DNA进行斑点杂交反应，通过观察杂交信号判断存在何种细菌感染，同时研究该方法的敏感性和特异性。2。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！