杂交

The response of the sensor to hybridization temperature and the specificity, reproducibility and sensitivity of the sensor were investigatied using Aloe-emodin as an electroactive indicator.

以电活性的芦荟大黄素为杂交指示剂，考察了杂交温度及传感器的特异性、重现性和检出限等性能。

The pollen living ability declined with time prolonging and the best condition for pollination was 22~28℃and fresh pollen appearing .By means of benzidine- hydrogen peroxide reaction and aniline blue fluorescence method, showed that the stigma receptivity could teach to 12 days since flowering. Compared with self pollination, the pollen was easily adhered into sigma and the pollen tube was easily entered into style in cross pollination experiment.

通过联苯胺—过氧化氢反应法和苯胺蓝荧光法观察了柱头的可授性、花粉在柱头的黏附、萌发及花粉管的生长情况，结果表明：非洲菊柱头发育初期就具有可授性，可授期达12d ；杂交和自交相比，杂交的花粉比较容易黏附、花粉管进入柱头比较容易，二者存在花粉黏附数量和花粉管进入数量的差异。

By Nothern blot, this gene band located between 1383-1908bp maker, length of which was same with different gene obtained by subtractive hybridization. The results showed that the obtained wnt6 gene was a cDNA fragment with full open reading frame, which could be useful for further studies.

提取样本总RNA，与地高辛标记的Wnt6探针进行杂Northernblot杂交，结果阳性杂交带位于1383-1908bp处，与差异片段大小一致，验证了该基因确为全长cDNA片段，具有完整的开放读框，可以用于进一步研究。

But the crossing combination of Asiatic hybrids ×Oriental hybrids and Longiflorum hybrids ×Oriental hybrids were not easy to success, there were two reasons: one was male parent was unfit for female parent, the other was cut-style pollination was unfit for these crossing combination, must used another method of pollination to try it again.

以亚洲百合为母本、东方百合为父本的杂交组合和以麝香百合为母本、东方百合为父本的杂交组合，座果率极低，推测有两种可能的原因：一是，所选父本的花粉对母本的亲合性较差；二是，切割柱头授粉的方法不适合于这些组合，应进行其它授粉方法的试验。

Taking 9 winter wheat species (4 mother plants and 5 father plants) and 20 hybridized combination using incomplete diallel crossing design as experimental materials, this study tried to find out the combining ability of amylose, amylopectin, total starch contents and swelling power in winter wheat species.

方法］以9个冬小麦品种（4个母本、5个父本）及其按不完全双列杂交设计配制的20个杂交组合为试材，研究冬小麦品种及F1籽粒直链淀粉、支链淀粉、总淀粉含量和膨胀势的配合力。

Label with LC-Red640 and LC-Red750 two are aimed at above all 112 code with 158 child detect 5 ′ carry bougie, corresponding anchor decides bougie to be labelled by fluorescence in 3 ′ end, circulate quickly from inside DNA of person gene group again PCR is distinctive the enlarge that make thing adds part of ApoE gene 265bP and with distinctive bougie cross hind, cause fluorescent resonance energy to transfer, the occurrence that passes different source cross and fault match alkaline base the fusible parameter of the type will finish feral model divide with choppy gene model.

首先以LC-Red640和LC-Red750标记两条针对112和158位编码子的检测探针5′端，相应的锚定探针在3′端被荧光标记，再从人基因组DNA中快速循环PCR特异引物扩增apoE基因265bP片段并和特异探针杂交后，引发荧光共振能量转移，通过异源杂交的出现和错配碱基的类型的熔解参数来完成野生型和突变基因分型。

The combinated utilization of ICH and ISH can greatly improve the detective efficiency of micro-metastasis in SLNs. The result detected by ISH arere, The ISH is more sensitive than the ICH method for the detection of micromeastasis.

通过免疫组化法和原位杂交法的联合使用，可明显提高乳腺癌SLN微转移的检出率，同时也证明原位杂交法是可靠的，比免疫组化更为敏感的技术。

Six crossbred steers (Simmental×Indigenous yellow cattle) with cannulas in rumen,duodenum and terminal ileum are used for determining the effects of feeding level on synthesis efficiency of rumen microbial protein.

用6头装有瘤胃、十二指肠、回肠瘘管的西门塔尔杂交阉牛，以Cr2O3为指示剂测定食糜流量，用嘌呤氧化法测定RNA当量，研究不同饲养水平下，西门塔尔杂交阉牛瘤胃微生物蛋白的合成效率。

Draining lymph nodes isolated from the BALB/C mice immunized with rabbit skin collagen Type Ⅲ accord- ing to a rapid immunization protocol, the lympho- cytes were fused with the myeloma cell line NS-1, 3 out of total of 201 growing hybridomas we obtained were producing collagen Type Ⅲ reactive antibodies after 3 limited dilution clones performed.

本实验采用快速免疫的方法用兔皮肤Ⅲ型胶原免疫BLAB/C小鼠，取淋巴结的细胞与小鼠骨髓瘤细胞NS-1融合，240孔中201孔有杂交瘤生长，用ELISA检测有6孔反应阳性。选3孔生长良好，抗体浓度高的细胞进行有效的有限稀释法克隆3次，获3株分泌抗Ⅲ型胶原单克隆抗体的杂交瘤细胞。其中二株属IgG2b，一株为IgG1。

Strong root activity in hybrid combinations is relatedto slower decrement in total amount of cytokinins.

杂交组合根系伤流液中细胞分裂素数量高于恢复系，杂交组合根系活力强，与细胞分裂素总量下降缓慢有关。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！