杂交

In conclusion, silkworm vasa plays an important role in the determination of the germ line.Whats more, the optimization of ISH has been tested.

此外，在进行原位杂交的研究中，我们探索和建立了家蚕胚胎原位杂交的方法，并对其中关键的影响因素进行了优化。

The results showed: after local leafhoppers were inoculated with RDV- SX isolate, RDV- LQ isolate, the vector viruliferous rates were similar as 84 % and 75% respectively,but the rice incidence rates were dissimilar as 28.1% and 2.8 % respectively.

研究表明：用本地黑尾叶蝉分别接种RDV本地分离物和云南分离物，斑点杂交显示介体带毒率相似，分别为84%、75%，但生物学接种结果差异相当大，分别为28.1%，3.8%，另外斑点杂交显示云南病区的黑尾叶蝉带毒率为88%，说明介体叶蝉的传毒能力具有地域性，介体叶蝉带毒率与传毒能力也存在一定差异。

Electrochemical cyclic voltammetry was employed to monitor the completion degree of hybridization, it was found that the the voltametric current was about 6 times increase under the microactuating with comparison of the non- microactuating.

用电化学循环伏安法监测其杂交程度，发现这种体积变化所致微点致动可引起DNA杂交度的很大增加，反映在伏安电流值上的变化是大约为无体积变化致动下的6倍。

Southern hybridization, Northern hybridization and enzyme activity determination all confirmed the effective expression of sorghum Sorghum vulgare L.

经Southern杂交、Northern杂交和酶活性检测表明，高粱C4型NADP-ME可以在水稻中有效表达，酶活性可被提高1～7倍。

Individuals crossing exclusively within the same population produce more progeny than those crossing in both directions and thereby use up some of their gametes for the production of inviable hybrids.

完全在同一种群内进行杂交的个体比以两个方向进行杂交的个体繁殖后代要多，因此为了产生无生机的杂种，就要消耗掉一些配子。

Crossbreedings with a few of the related mannikin species (Shaft-Tailed Finches or White-Backed Munias, for example) can produce fertile offspring, but this should be discouraged.

与那些mannikins亚属种类的杂交后代具繁殖能力，但是应当阻止这种杂交。

Female parent group was made up of B,C and D population,including 9 high-starch entries.

在按不完全双列杂交设计，组配出了72个杂交组合。

Objective To study the technique and diagnostic value of fluorescence insitu hybridization in chromosome abnormality for prenatal diagnosis. Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium.

荧光原位杂交(fluorescence in situ hybridization， FISH)是分子杂交与组织化学相结合的一项新技术，近年来用于人类染色体分析，它能快速、准确地确定在传统方法中无法判别的染色体异常，特别对来源不明的标记染色体能作出正确诊断［1，2］。

Second Municipal People's Hospital of Guangzhou, Guangzhou 510150 Objective To study the technique and diagnostic value of fluorescence insitu hybridization in chromosome abnormality for prenatal diagnosis. Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium.

对34例孕16～23周有产前诊断指征者经腹部抽取羊水，用Chang培养液传代培养，常规制备羊水细胞分裂中期染色体，应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针，与培养的羊水细胞分裂中期染色体DNA进行原位杂交，杂交后用荧光显微镜观察玻片并摄像。

Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium. The metaphase chromosomes were hybridized insitu with the human centromere probes,α-satellites DNA probes of X, Y,13, 21, 18 chromosomes and all primer chromosome probes. These probes were conjugated by Biotin and Dioxin then. The treated slides were examined and taken photos under the fluoromicroscope.

对34例孕16～23周有产前诊断指征者经腹部抽取羊水，用Chang培养液传代培养，常规制备羊水细胞分裂中期染色体，应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针，与培养的羊水细胞分裂中期染色体DNA进行原位杂交，杂交后用荧光显微镜观察玻片并摄像。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！