杂交

Parthenocarpic lines and 2 non-parthenocarpic lines of cucumber were crossed by half mating design. The rate of parthenocarpic fruit set of parent and F1 were checked before flowered by clutching flowers.

选取黄瓜单性结实性差异显著的4个材料，采用完全双列杂交半轮配试验设计配制杂交组合，利用开花前掐花的方法测定亲本及F1单性结实坐果率。

The immunohistochemistry showed that accompanied with SDNSF mRNA upregulation, the nestin-positive cells showed erupted roots, migrated peripherad and proliferation on the 8-day slice.

结果 RT-PCR检测SDNSF mRNA在正常大鼠脊髓中的表达，损伤后4天SDNSF 的mRNA表达上升，损伤8天到达高峰，此后SDNSF 的mRNA表达逐渐减少，到16天恢复到正常水平；脊髓切片原位杂交结果发现SDNSF的 mRNA 阳性细胞主要分布于脊髓灰质细胞中，可能是神经元细胞，结果表明正常脊髓可表达SDNSF；脊髓损伤后8天，原位杂交显示SDNSF阳性细胞明显增多。

The immunohistochemistry showed that accompanied with SDNSF mRNA upregulation, the nestin-positive cells showed erupted roots, migrated peripherad and proliferation onthe 8-day slice.

结果 RT-PCR检测SDNSF mRNA在正常大鼠脊髓中的表达，损伤后4天SDNSF的mRNA表达上升，损伤8天到达高峰，此后SDNSF的mRNA表达逐渐减少，到16天恢复到正常水平；脊髓切片原位杂交结果发现SDNSF的mRNA阳性细胞主要分布于脊髓灰质细胞中，可能是神经元细胞，结果表明正常脊髓可表达SDNSF；脊髓损伤后8天，原位杂交显示SDNSF阳性细胞明显增多。

Besides,two fertile hybrids were obtained from symmetric somatic hybridization between bread wheat and tall wheatgrass,both of which were phenotypically similar to the tall wheatgrass parent.

本实验室成功的进行了小麦与多种禾草的体细胞杂交，并在小麦与长穗偃麦草的对称与不对称体细胞杂交中分别获得了长穗偃麦草/小麦渐渗系和小麦/长穗偃麦草渐渗系。

In an attempt to promote the efficiency in citrus breeding and estimate the pos- sibility of embryo culture applied to citrus production,we carried out the culture technique in the hybrid embryos(Citrus sinensis Osbeek×Poncirus trifoliata Raf.).

为了提高柑桔杂交育种效率和探讨胚培养在柑桔生产上应用的可能性，我们研究了锦橙×枳(Poncirus trifoliata Raf。)的杂交种子胚培养技术。证明：怀特培养基+IAA(1ppm)+CH(600ppm)和 MT 培养基+GA(1ppm)能使第五个小胚萌发成苗；在 MT 培养基+2，4-D(0.7ppm)+BA(0.23ppm)上，有些胚先形成愈伤组织，然后从原有的胚中生根出苗。

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电泳转印槽组件 1。缓冲液槽及带有电缆的盖 2。凝胶支架转印夹 3。纤维衬垫 4。电极丝 5。电极板 6。特级冷却芯 Trans-Blot 转印槽是功能灵活的转印设备，可理想地用于多种转印应用。Trans-Blot 转印槽特点包括：*能进行多胶转印，可容纳3 块PROTEAN I xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶*多组参数灵活可设，可调节的电压设置（从30 V 的过夜转印到200 V 的1 小时快速实验）*电极间距设置为8 cm 用于标准印迹杂交，或设置为4cm 用于高强度印迹杂交*可选择板式电极：涂有铂金的钛作为正极，不锈钢为负极，能提供高强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝*通过特级冷却芯和水循环仪来调节温度―是天然酶（4°C）或高强度转印的理想选择，随着转印时间增加（多达24 小时），不会引起缓冲液耗竭（在高强度转印中必须使用冷却芯，也推荐用于所有板式电极的应用）*带铰链的凝胶支架转印夹能避免滑动，确保凝胶与印迹膜间的紧密接触；每个转印夹都有颜色标记以保证在转印槽中的正确定位 Trans-Blot 转印槽的锁闭凝胶支架转印夹系统。转印夹（1）支撑凝胶（2）印迹膜（3）两侧有纤维衬垫和滤纸（4）确保凝胶三明治内的完全接触。凝胶夹垂直插入缓冲液槽中（5）。

It yielded 6.44% and 13.94% higher than the check hybrid 9 You 138 in the regional yield trials and the productional trial of Jiangsu Province, respectively.

徐优201（徐9320A 徐恢201）是一个优质高产杂交中粳新组合。20092004年参加江苏省杂交中粳区试和生产试验，2a区试平均比对照9优138增产6.44％，生产试验比对照增产13.94％。

To investigate the possibility of such a transition, we studied 17 cases of MGA or atypical MGA some of which had given rise to carcinoma in situ and/or invasive ductal carcinoma using the reticulin stain, immunohistochemistry (S-100, p63, Ki-67, and p53), and a molecular approach involving microdissection and high-resolution comparative genomic hybridization and MYC chromogenic in situ hybridization.

为了研究这种转变的可能性，我们分析了17例典型或非典型的MGA，其中一些已经发展成为原位癌和/或浸润性导管癌，所用的方法是网状染色、免疫组化（S-100， p63， Ki-67， and p53）和分子技术，分子技术包括显微切割和高分辨率比较基因组杂交及MYC显色的原位杂交。

The experiment showed that the twin spirochaeta of PNA-DNA can quench the fluorescence of ST in some degree.And the fluorescence quenching of ST was reduced when PNA-DNA twin spirochaeta dissociate.

实验结果发现当 PNA- DNA杂交形成双链时，可以在一定程度上猝灭 ST的荧光，而当双螺旋解链时，对 ST荧光的猝灭程度减小，逐渐接近于 ST本身的荧光，根据荧光强度的变化研究了 PNA- DNA的杂交和解链过程。

To enhance computational efficiency, mechanical subelement model is incorporated, for simulating material hardening properties, in the hybrid/mixed finite element analysis of finite deformation problems.

为了提高计算效率，本文首次把力学子单元模型引入有限变形杂交/混合有限元分析工作，用以简化弹塑性本构模型。数值分析表明，在有限变形杂交/混合有限元分析工作中使用该模型，能有效地提高计算效率。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！