杂交

Methods Cultivation of alveolar type Ⅱ cells were mode from normal adult rat and of silicotic animal model of rat was established.

分离培养正常成年大鼠肺泡Ⅱ型细胞，建立大鼠矽肺模型，用原位杂交和免疫组化技术检测体外培养的和矽肺病变中的肺泡Ⅱ型细胞TGFβ1和PDGF BmRNA和蛋白的表达。

Methods Cultivation of alveolar type Ⅱ cells were mode from normal adult rat and of silicotic animal model of rat was established. In situ hybridization and irnmunohistochemieal staining were applied to detect the gene expression of TGFβ1 and PDGF-B in the alveolar type Ⅱ cells of primary culture and silicotic tissue.

分离培养正常成年大鼠肺泡Ⅱ型细胞，建立大鼠矽肺模型，用原位杂交和免疫组化技术检测体外培养的和矽肺病变中的肺泡Ⅱ型细胞TGFβ1和PDGF-B mRNA和蛋白的表达。

Concentrations of DA and 5-HT in different regions were measured by high performance liquid chromatography; the expression of SERTmRNA was detected by in situ hybridization; the degeneration of neuron and terminal was demonstrated by Bielschowsky and Glee Marsland silver staining; and the protein expression of GFAP was detected by immunohistochemistry.

2采用高效液相色谱法测定不同脑区神经递质DA和5-HT的含量；采用原位杂交方法检测SERTmRNA；Bielschowsky及Glee Marsland氏改良银浸染法显示神经元胞体、树突和轴突；免疫组织化学方法检测GFAP的表达。

We expect to see similar figures for the Ram Hybrid.

我们希望看到类似的数字公羊杂交。

Compared with hybrid transferring breeding, the radiation induced-mutation had advantages of simple method and short cycle.

辐射诱变与杂交转育方法相比，具有方法简单、周期短的优点。

In situs hybridization histochemical technique is applied to detect the expression of five subtype of human somatostatin receptor Mrna (hSSTR1-5), including the expression model of 54 cases of various tumor specimen and the expression density of 24 lung cancer cases.

本研究采用原位杂交组织化学技术，探测高度表达人生长激素抑制素受体的5种亚型(hSSTR1-5)的54例肿瘤标本，包括54例肿瘤SSTR亚型的表达模式及其中24例肺癌的表达密度。

Basic Study on Erection Planning Method for Beam String Structure 2,Study on Structural Characteristic of Hybrid Skeleton with Tension Strings 3, Proposal of Reversed Progress Teclmique for Tension Structures and Its Application 4,Mechanical Behavior of Hybrid Skeleton with Tension String during Construction 5,Experimental Studies on Structurel Characteristic of Hybrid Skeleton with Tension Strings 6,Mechanical analysis of a type hybrid spatiaI structure composed of cables,bars and beams 7, Tensile force compensation analysis method and application in construction for hybrid structures 8,Tensile forces emulationaI analysis of prestressed cables for multistage tension construction method 9,Reverse analysis method of construction centrel tensile force for prestressed space structures

1 ，基础研究规划方法架设张弦梁结构 2 ，结构特征研究混合骨架张力弦 3 ，建议反进展Teclmique张力结构及其应用 4 ，力学性能的混合骨架张力字符串施工 5 ，实验研究Structurel特征混合骨架张力弦 6 ，机械分析型杂交spatiaI结构组成的电缆，酒吧和梁 7 ，张力补偿的分析方法及应用在建筑混合结构 8 ，拉伸部队emulationaI分析的多级预应力锚索张拉施工方法 9 ，逆向分析方法的建设centrel张力的预应力空间结构

Hybrid snakehead Larval were stocked and reared in an outdoor tank at a rate of0.5, 1, 2, 4 and 8 ind/L for 15 days.

以全长1.00 cm的刚开口杂交鳢仔鱼为研究对象，研究了五种养殖密度(0.5、1、2、4、8尾／L)对其生长、存活的影响，实验持续15 d。

The Triangle town of the agricultural sector and seed breeding of aquatic products in the relevant market with the help of fisheries experts, for the first time in Guangdong Province will be carried out and the area north of Hunan and Hubei and the snakehead Channa spot of Hong Kong to carry out research crossbreeding.

2000年起，三角镇农业部门和裕荣水产种苗繁育场一起在相关水产专家的协助下，首次在广东省开展了将两湖及以北地区的乌鳢与香港地区的斑鳢进行杂交育种的研究工作。

Theexperimental results were as follows: 1. The effects of prey density (0.1, 0.3, 1, 3 and 10 ind/mL) on growth and survivalin different body sizes (0.68, 1.50, 3.20 cm) of hybrid snakehead larval were investigated.

本实验针对杂交鳢苗种培育存活率普遍偏低的状况，对影响其生长、存活的饵料密度、放养密度和残食三个关键因素做了深入研究，试验结果如下： 1。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。