杂交

Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示，银鲫pou2基因有母源转录本，其合子基因在高囊胚期强烈表达，在50%下包期和90%下包期也有高量的转录本，但在100%下包期表达量急剧降低，至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

The low yield in seed production is an important limiting factor for the development of japonica hybrid rice, and the stigma exsertion rate of male sterile lines has a key effect on seed yield of japonica hybrid rice.

制种产量低是制约杂交粳稻发展的重要因素，而不育系柱头外露率是影响杂交粳稻制种产量的关键。

Since 1996, the Rice Research Institute of Liaoning Province has laid stress on the genetic improvement of stigma exsertion rate of japonica male sterile lines. By introducing the genes of high stigma exsertion rate from indica into japonica, a group of japonica male sterile lines with high stigma exsertion rate have been successfully developed. Their stigma exsertion rate have been greatly increased from less than 30% to more than 80%, resulting in an increased outcrossing rate over 60% and a high seed production yield of 3.0~4.5 t/hm^2. Moreover, using these sterile lines, a group of new japonica hybrid rice combinations such as Liaoyou 5218 (Liao 5216A/C418), Liaoyou 1052 (Liao 105A/C52) have been developed and released to commercial production.

自1996年以来，辽宁省稻作研究所加强了粳稻不育系的柱头外露率遗传改良研究，通过导入籼稻的高柱头外露率基因，选育了一批高柱头外露率的粳型不育系，柱头外露率由原来的30%以下逐步提高到80%以上，异交结实率可达到60%以上，杂交制种产量可提高到3.0~4.5t/hm^2，并配组育成了辽优5218（辽5216A/C418）、辽优1052（辽105A/C52）等杂交粳稻新组合，在生产上得到了大面积推广应用。

In test group fibroblast growth factor immunoreactivity was detected in 89% of the samples, mRNA was detected in 20% of the samples. In control group no fibroblast growth factor immunoreactivity and mRNA was detected.

结果：实验组30例免疫组化和原位杂交的阳性率分别为89％和20％，对照组6例免疫组化和原位杂交均为阴性，两组免疫组化方法检测阳性率有显著差异。

In terms of raising theoretical reference, techniques of cross breeding ,selection of new cultivars in Gerbera jamesonii have been preliminary studied .

本文在花器发育、授粉生物学、杂交技术、优良品系选育等方面进行了一些研究，同时进行了部分性状分离变异规律的初探，以期为杂交育种工作提供参考。

The male-sterile line improve should by the Shallu and the Kafir or the Shallu hybrid primarily, the r-line improve should by the kaoliang and Hegari or the kaoliang hybrid primarily, may maintain two kisses the heredity difference, in order to compounds the high yield hybrid.

不育系改良应以印度高粱与南非高粱或印度高粱杂交为主，恢复系改良应以中国高粱与亨加利高粱或中国高粱杂交为主，这样可保持两亲间的遗传差异，以便配制出高产杂交种。

Vacuoles and black dense particles usually occurred in cytoplasm of heterokaryon, and occasionally, swelling of mitochondria, pycnotic nuclei or even enucleation process could be seen among hybrid cells.

超微结构分析表明，杂交细胞表面膜微绒毛、指状突及皱褶突等均明显减少，核内异染色质比例增高，核仁数目减少或消失，细胞质中常可见有空泡或黑色致密颗粒出现；少数杂交细胞出现线粒体肿胀、核固缩甚至排核现象。

Compared with D buffalo, the withers height, hip croop height, hucklebone width of ND buffalo respectively increase 5.5%, 7.4%, 9%, the withers height, hip croop height, hucklebone width of MD buffalo respectively increase

结果显示，杂交水牛尼本的鬐甲高、十字部高、坐骨节宽比德宏水牛分别提高5.5%、7.4%、9%，差异显著。杂交水牛摩本的鬐甲高、十字部高、坐骨节宽比德宏水牛提高5.9%、8.0%、11.9%，差异显著。

So the technology of introduction of exogenous DNA into tomato after pollination not only feasible within species but also feasible in over-distance hybridism and excellent gene can been transferred to the cultivate variety.

试验还选用野生种醋栗番茄作供体进行了远缘关系的分子杂交试验，以克服杨晓锋：对外源DNA导入番茄的研究远缘杂交不亲和性，将野生种抗病性强的优良性状转移到受体中来，重组优良基因培育新品种材料也获得了较为满意的效果。

Balb/c mice were immunized with the purified PRRSV - BJ isolate. mouse spleen cell were fused with myelomas NSo .culture supernatants of hybridism's were screed by indirect ELISA. Positive colonies were cloned three times by limited dilution. One cell line named 6PR, which could secret McAb stably was obtained.

用纯化的猪繁殖与呼吸系统综合征病毒免疫Balb/c小鼠，应用淋巴细胞杂交瘤技术，取脾细胞与NS0骨髓瘤细胞融合，经间接ELISA筛选，三次有限稀释法克隆，得到一株能稳定分泌抗猪繁殖与呼吸系统综合征病毒单克隆抗体的杂交瘤细胞株：6PR。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。