杀虫的

C1oning ofinsecticidal novel cry\' genes to Coleopteran pest tYom Bacillus lhuringiensisisolates will be beneficial fOr app1ication of pesticidal novel gene resources andwill lessen the gap betWeen domestic and abroad.

从Bt菌中分离克隆杀鞘翅目害虫的特异基因，将有效开发利用我国丰富的杀虫基因资源，缩小国内外在Bt杀虫基因基础研究领域中的差距，为工程菌和抗虫转基因植物的研制提供新的基因来源。

In this paper, we determined the toxicity of transgenic Betula platyphylla with Bt spider peptide gene. Larvae of Lymantria dispar were feed on leaves of transgenic Betula platyphylla with Bt spider peptide gene, The mortality, reduced ratio of weight, ecdysis index and schedule time of every instar.

本文对转Bt C肽蜘蛛杀虫肽的白桦进行杀虫性测定，对取食对照和转基因植株舞毒蛾幼虫的死亡率、体重降低率、蜕皮指数、幼虫各龄级历期等指标进行了分析，证明外源基因已经成功插入白桦基因组中，并且能够表达杀虫蛋白。

The fused insecticidal protein may delay the process of insects?developing resistance. After investigation of the secondary structure and action mode of Bt insecticidal protein and Cowpea trypsin proteinase inhibitor, as well as the action mechanism of insect enterokinase, we devised the fusion gene on the base of maintaining their functions respectively. The method follows: CpTI gene is linked to 3?-end of GFM crylA gene by a nucleotide sequence encoding cleavage site of insect enterokinase.

根据Bt杀虫蛋白Cry1A和豇豆胰蛋白酶抑制剂的高级结构、杀虫机理及昆虫体内肠激酶的作用机理，本着尽量不影响二者功能的原则，设计出了构建Cry1A和CpTI融合杀虫基因CryCI的方案，即将CpTI基因的编码序列连接到GFM Cry1A基因编码区3'端，并在其间用一段编码肠激酶切割位点的序列相连。

The results of HPLC tests indicated that Bt toxin in Bt transgenic cotton had not obviously affected the contents of flavonoids (mainly rutin and isoquercitrin and a few quercetin ),contradictively ,the result of combined dosage test in vitro showed that that rutin had significantly enhanced the suppression of Bt-ICP on the cotton bollworm larvae., in addition to the

采用高效液相色谱法测定转Bt基因棉花主要抗虫黄酮类化合物的的变化，结果表明外源Bt杀虫蛋白表达不会对棉花自身原有的主要抗虫黄酮类物质（包括芸香苷、异槲皮苷和含量较少的槲皮素）产生不利的影响，因此可以将Bt杀虫蛋白基因转入抗虫黄酮类物质中国农业科学院博士学位论文含量高的棉花品系。

This thesis introduces the domestic and international research development on techniques of preventing against and killing archives pests briefly at first, on the basis of advantages and disadvantages of chemical 、 physical、 biological pest-killing techniques and practice of realistic archives preservation,determines to research lethiferous effectiveness of high temperature pest-killing technique、 low temperature pest-killing technique、 microwave pest-killing technique and high pressure nitrogen suffocation pest-killing technique,and affect on durability of making materials of paper archives systemically.

本论文首先简要介绍了国内外档案害虫防治技术研究进展，在分析化学防治法、物理防治法和生物防治法的优缺点及现实档案保管工作实践的基础上，确立系统研究高温杀虫法、低温杀虫法、微波杀虫法、高压充氮杀虫法的杀虫效力及对纸质档案制成材料耐久性的影响。

This thesis analysis lethiferous effectiveness of high temperature pest-killing technique、 low temperature pest-killing technique、 microwave pest-killing technique and high pressure nitrogen suffocation pest-killing technique, and affect on tensile strength、 wearproof strength、 whiteness of copypaper、 writing paper、 rice paper and newsprint, affect on colour difference of red 、 blue 、 blue-black ink colored writing, and black ink 、 laser printing 、 xerox,、 gushing typing black writing caused by four physical pest-killing techniques above-mentioned.

本论文系统分析了高温杀虫法、低温杀虫法、微波杀虫法和高压充氮杀虫法的杀虫效力，并在档案保护技术学领域较系统地探讨了四种物理法杀虫处理对复印纸、书写纸、宣纸、新闻纸的抗张强度、耐破度、白度的影响，以及对红、蓝、蓝黑墨水彩色字迹，黑墨水、激光打印、静电复印、喷墨打印黑色字迹色差的影响。

The fumigation effect of the essential oil with and without HCN of bitter almond on insects was studied. The results show that the two kinds of bitter almond oil have strong fumigation activity against Musca domestica vicina, Aedes albopictus and Mythirmna separata. It is have the characteristic of low effective concentration and quick response.

通过含有HCN和去除HCN的苦杏仁精油对四种试虫的熏蒸杀虫活性，表明含有HCN和除去HCN两种苦杏仁精油对家蝇、白纹伊蚊以及粘虫均具有很强的熏蒸杀虫活性，具有作用浓度低、作用时间快的特点。

The main influence factors yn sulphonation and crystallization process has been investigated and the optimum conditions of process have been foudn.

摘 要：介绍了杀虫单的介质法磺化反应及结晶工艺的研究，探讨了影响磺化收率和杀虫单粉剂含量及出粉率的主要因素，找出了生产高纯度杀虫单粉剂的最优工艺条件。

The results showed that 3 acaricides, pyridaben, propargite, hexythiazox and 6 insecticides, abamectin, chlorfenapyr, cyhalothrin, emamectin benzoate, oxymatrine prosuler, envidor had good control effects and there was no significant difference among them. It had excellent control effects for 4 low-toxicity insecticides including propargite, hexythiazox, abamectin, and emamectin benzoate.

结果表明，3种专一性杀螨剂哒螨灵、克螨特、噻螨酮及6种广谱性杀虫杀螨剂阿维菌素、虫螨腈、高效氟氯氰菊酯、甲维盐、氧苦内脂和螺螨酯对叶螨均有良好的防治效果，彼此之间差异不显著。

Theresults show that the tempreture changes can affect the growth of the transgenic cotton and theexpression of the Bt insecticidal gene and therefore affect the timing and the onsetting of thetransgene silencing. The time of the occurrence of silencing is more earlier after hightemperature treatment.

结果表明，温度变化影响抗虫棉早期的生长发育和Bt杀虫基因的表达，从而影响Bt杀虫基因沉默的发生时间，前期高温处理可使Bt杀虫基因在生长发育期中的沉默时间提前。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。