杀细胞的

They found that Pu'er Tea has a strong function in killing the cancer cells, even centesimal density of the ordinary persons very obvious.

梁明达、胡美英教授用细胞培养及电子显微镜方法，对普洱茶的抗癌作用进行了十多年的研究，发现普洱茶杀癌细胞的作用最为强烈，甚至常人喝茶百分之一的浓度即有明显作用。

Prevent and resist cancer. Professor Liang Mingda and Hu Meiying studied anti-cancer function of Pu'er Tea by cell culturing and electronic microscopic methods for more than ten years. They found that Pu'er Tea has a strong function in killing the cancer cells, even centesimal density of the ordinary persons very obvious.

防癌、抗癌。梁明达、胡美英教授用细胞培养及电子显微镜方法，对普洱茶的抗癌作用进行了十多年的研究，发现普洱茶杀癌细胞的作用最为强烈，甚至常人喝茶百分之一的浓度即有明显作用。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

Cytokine-induced killercells are a cluster of alloplasm cells,which are prepared from the mononuclear cells of human peripheral blood that might be stimulated by some kinds of cytokines.The proliferation and cytotoxicity of CIK cells are high,and the side effect is low.CIK cell is a more effective anti-tumor effector cell in adoptive immunotherapy.

细胞因子诱导的杀伤细胞是人外周血单个核细胞在体外经多种细胞因子刺激后获得的一群异质细胞，它具有增殖能力强、杀瘤活性高和杀瘤谱广、临床应用不良反应小的特点，是肿瘤过继免疫治疗中更为有效的杀瘤效应细胞。

CIK cells were highly efficient cytolytic effector cells which were non-MHC restricted.

CIK细胞是一种强于LAK细胞的、新型、高效、具有广谱杀瘤活力的免疫活性细胞。

Patchouli also has cell-regeneration properties and also has fungicidal properties.

广霍香还有促进细胞再生和杀霉菌的功能。

Based on these results and inferred to related reports from other labratories, it was possible to make some analyses and conclusions or inferrences:(1) CD〓AK with tumoricidal activity were induced and expanded in number througth costimulation of PBMC with anti-CD〓 McAb . and r IL-2 ;(2) CD〓AK induced and expanded in such manner did exibit more potent proliferation·ability and cytotoxicity which maintained for lonser time than those of LAK cells, thus CD〓AK was a new variety of antitumor effector cells worth to be explored;(3) CD〓AK could mediate MHC nonrestricted cytotoxicity and kill tumor target cells through inducing necrosis and apoptosis;(4) Normal mature lymphocytes of PBMC could be induced to proliferate and /or to die from apoptosis when they were costimulated by anti-CD〓McAb and rIL-2. Both proliferation and apoptosis were existing in the same cultivation system sugsesting that the presence of rIL- 2 might provide some accessary signals for apoptosis.

以这些结果为基础并参考其它有关文献可能做出如下分析与结论或推论：(1)用抗CD〓单抗和rIL-2共刺激外周血单个核细胞能诱生扩增出具有杀瘤活性的CD〓AK细胞，(2)与LAK相比，用这种方法诱生扩增的CD〓AK增殖能力强、细胞毒活性强而且维持时间长；CD〓AK是一类值得开发的抗瘤效应细胞；(3)CD〓AK能够介导MHC非限制性细胞毒活性，可以通过诱导靶细胞坏死和/或凋亡杀伤肿瘤细胞；(4)正常外周血单个核细胞中的成熟淋巴细胞在受到抗CD〓单抗和rIL-2共同刺激后既可诱导增殖也可诱导凋亡，两者并存于同一体系，推测rIL-2的存在可能为细胞凋亡提供一些辅助信号。

Objective To study the optimal irradiation time and power density in photodynamic therapy with delta-aminolevulinic acid for tumor cells photoinactivation in vitro.

目的 探讨5-氨基酮戊酸光动力学疗法体外伤杀肿瘤细胞的最佳照光时机和最佳照光能量。

Results Compared with solvent control,toxaphene increased significantly MN frequencies in Hep G2 cells at concentrations of 20,40?μmol/L respectively(P.01,P.01).No significant increase of MN frequencies were found in Hep G2 cells treated with Aroclor1254(23～184?μmol/L) and DDT17.8～60?

结果20，40μmol/L毒杀芬处理Hep G2细胞的微核率与溶剂对照相比显著增加(P.01，P.01)；经Aroclor1254(23~184μmol/L)和滴滴涕(17.8~60μmol/L)处理的Hep G2细胞的微核率与溶剂对照相比，差异无统计学意义。

When the cytotoxic activity of these two types of TNF was compared, the synthesized 26kD TNF was found to exert its effects only in the presence of microsomal membranes.

比较这两种体外表达产物对靶细胞的细胞毒效应，结果表明，只有在微粒体存在时所合成的26kD TNF具有细胞毒活性；而无微粒体存在所合成的裸26kD TNF不显示杀瘤效应。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。