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The resources and pathogenicity of entomogenous fungi in Lishan National Nature Reserve in the southern east of Shanxi Province were studied.

对地处山西东南部的历山国家级自然保护区的虫生真菌种类资源及其杀虫活性作了初步的研究。

It was then introduced by gene pulser into acrystalliferous strain CryB and a recombinant strain CryB (pBMBZGC10) was obtained. Different fermentative solutions of recombinant strain were used for multi-spray on leaves of Brassica pekinesis, Ipomoea aquatica and Lycopersicon esculentum. Results by using fluorescent detection and PCR amplification revealed that cry1Ac10 gene could not been detected in roots, stems and leaves of plants tested. The cry1Ac10 gene was also not transferred into indigenous bacteria, actinomyces and fungi in soil.

苏云金芽胞杆菌标记重组菌株与杀虫基因水平转移的研究将重组菌株CryB(pBMBZGC10)的发酵液按浓度梯度分次进行喷洒小白菜、蕹菜和番茄作为供试植株,利用标记基因研究的结果表明:cry1Ac10基因没有向供试土壤细菌、真菌和放线菌转移,也未在植物的供试植物根、茎和叶中检测到该基因。

The bioassay results showed that the quaternary ammonium salt had a good intihtion against typical microorganisms such as heterotrophic bacteria,ammonifying bacteria,denitrifying bacteria and nitrosation bacteria with its inhibition rate was above 90%. Inhibition of the quaternary ammonium salt to fungi and sulfate reducting bacteria was above 85% after 30 min. Inhibition of the quaternary ammonium salt to black mold was about 84% after 30 min,and the to algae was totally inhibited after 24 h.

高分子抗菌杀生剂的抗菌灭藻性能的检测结果表明,作用30 min对贵阳市雅河水中的异养菌、氨化细菌、反硝化细菌、亚硝化细菌的杀菌率在90%以上,真菌、硫酸盐还原菌的杀菌率在85%以上;对黑曲霉的杀菌率在84%,其作用24 h可将小球藻基本杀灭。

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