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Multocida capsular serogroup A strains, and might be a useful vaccine candidate against fowl cholera.

粘附蛋白Cp39是禽多杀性巴氏杆菌荚膜蛋白中的主要交叉保护抗原,可以作为禽霍乱亚单位疫苗。

Strains containing cry1 genes were the most abundant in our collection (66%), and 21 different cry1-type gene combinations were found. Furthermore, several novel holotype cry genes were found and the full-length sequences of 3 novel cry genes were designated as cry54Aa1, cry30Fa1, and cry30Ga1 by B.

PCR-RFLP鉴定结果表明:此地区的苏云金芽胞杆菌主要含有cry1,cry2,cry3,cry4/10,cry9,cry30和 cry40 等7种cry基因类型;含cry1基因的菌株最丰富,共有21种不同cry1型基因组合;从中发现了新型模式基因,并采用Tail-PCR技术获得了其中3个基因的全长序列,被国际苏云金芽胞杆菌杀虫晶体蛋白基因命名委员会命名为cry54Aa1、cry30Fa1和cry30Ga1。

To evaluate the diversity of immunogenicity of IROMPs, OMPs and whole-cell bacterin of leporid P. multocida, and evaluate their cross protection in mouse model.

本试验以小鼠为动物模型,比较了兔多杀性巴氏杆菌铁调节外膜蛋白的免疫原性及分析交叉免疫保护效果。

But the research about the relation between production of ICPs and sporulation genes is less.

本研究利用苏云金芽胞杆菌全基因组序列,初步研究了芽胞形成相关基因与杀虫蛋白基因表达、调控的相互关系。

Different plant expression vectors had been respectively transferred into upland cotton cultivars Jinman12, Jinmian7, Xinluzao1 and Jihe321 via Agrobacterium tumfaciens transformation. These vectors carried cryIAc3 gene, which encodes Bacillus thuringiensis δ-endotoxin, under the control of chimeric OM promoter, CaMV 35S promoter, and cotton leaf curl virus RP promoter respectively; snowdrop lectin gene (Galanthus nivals agglutinin, gna) trans-regulated by rolC promoter controlled TGAla factor; and multivalent expression construct of both cryIAc3-cpti fusion gene and gna gene. A large number of transgenic plants and their progenies had been obtained.

为了提高外源基因的表达量,延缓害虫产生耐受性,本文通过将携带有分别在高效复合OM启动子,CaMV35S启动子及棉花曲叶病毒RP启动子控制下的苏云金杆菌杀虫毒蛋白基因cryIAc3;反式调节因子增强韧皮部表达的雪花莲外源凝集素(Galanthus nivals agglutinin,GNA)基因;以及同时含有cryIAc3、豇豆胰蛋白酶抑制剂基因和gna三价抗虫基因的植物表达载体,以根农杆菌介导法分别将这些表达载体导入了陆地棉新陆早1号、晋棉7号、晋棉12号和冀合321等我国西北棉花主要栽培品种,经体细胞胚再生获得了大批转基因再生植株。

Mixtures of the final whole cultures of recombinant strains Bt B601, Bt B611, Bt B640, Bt U 30(expressing Cry4Aa, Cry4Ba, Cry11Aa and Cyt1Aa protein of Bacillus thurigiensis subsp. israelensis respectively) and Bt CW 3(expressing the Binary toxin of Bacillus sphaericus ) were used in bioassays against resistant Cpq larvae to value the synergism among the mosquito larvicidal toxins.

本研究测定了分别表达苏云金芽孢杆菌Cry4Aa、Cry4Ba、Cry11Aa、Cyt1Aa和球形芽孢杆菌二元毒素Bin的转化菌株Bt B60 1、Bt B611、Bt B640、Bt U 30和Bt CW 3全发酵培养物两两或两两以上不同组合对抗性库蚊的毒力,分析了杀蚊毒素间的协同作用。

At present it is widely accepted that the damages of the nasal turbinate bones and mucus caused by Bordetella bronchiseptica laid the foundation for the invasion toxigenic Pasteurella multocida. The mixture of Bordetella bronchiseptica and toxigenic Pasteurella multocida, together with some other factors, is the main cause of natural AR.

经过多年研究,现在大多数学者认为,支气管败血波氏杆菌能引起一定程度的鼻粘膜和鼻甲骨损害,但这些病变的严重后果是为正常猪鼻腔不能定居的产毒素多杀巴氏杆菌建立感染铺平道路,Bb和T+Pm的混合感染加上非传染因素交织在一起,是在自然条件下临床型AR的发生原因。

The minimal bactericidal concentration of PTKE on Escherichia coli, Staphylococcus aureus, Salmonella, Pasteurella multocida and Streptococcus agalactiae were 100, 25, 50, 25 and 50 mg/mL respectively. The isolation of EA, EB and EC had no antibacterial effect on Escherichia coli and Pasteurella multocida. MBC of EA on Staphylococcus aureus and Salmonella were 2.5 and 5 mg/mL, and MBC of EB on Staphylococcus aureus, Salmonella and Streptococcus agalactiae were 1.25, 5 and 2.5 mg/mL respectively, while MBC of EC on Staphylococcus aureus was 2.5 mg/mL. Polygonum taibaishanense Kung.

PTKE对大肠埃希菌、金黄色葡萄球菌、沙门氏菌、多杀性巴氏杆菌和无乳链球菌的最低杀菌浓度分别为100,25,50,25和50 mg/mL;而PTKE的分离物EA、EB和EC对大肠埃希菌和巴氏杆菌无抑菌作用,EA对金黄色葡萄球菌、沙门氏菌的MBC分别为2.5和5.0 mg/mL,EB对金黄色葡萄球菌、沙门氏菌和无乳链球菌的MBC分别为1.25,5.0和2.5 mg/mL,EC对金黄色葡萄球菌的MBC为2.5 mg/mL。

Pathogeny of northeast tiger was separated and identified in Jilin City Jiangnan Park. In order to carry out the epidemiological investigation and provide the accuracy diagnose and treatment measures, toxA gene of pastuerella was researched in the experiment.

通过对吉林市江南公园送检东北虎病例的病原分离鉴定,分离出多杀性巴氏杆菌,为进一步加强对该病的流行病学调查,并为准确诊断和治疗该病提供依据,实验对该株巴氏杆菌毒力基因toxA进行了研究。

The present invention discloses a strain of broad spectrum bacillus thuringiensis, Hcy08-02, preserved in the Chinese typical culture article preserving center in Wuhan University with the CCTCC No.M202025. It has excellent fermentation property, contains several pesticidal protein encoding genes and has effective killing function to cotton bollworm, cabbage moth, small mottid willow moth, cabbage worm and other pests.

本发明公开了一株广谱苏云金芽孢杆菌Hcy08-02,在武汉大学中国典型培养物保藏中心保藏,编号:CCTCC NO:M202025,该菌株有优良的发酵性,含有多个杀虫蛋白质编码基因,对鳞翅目的棉铃虫、小菜蛾、甜菜夜蛾、菜青虫等害虫具有高效的毒杀作用。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。