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In this paper, we determined the toxicity of transgenic Betula platyphylla with Bt spider peptide gene. Larvae of Lymantria dispar were feed on leaves of transgenic Betula platyphylla with Bt spider peptide gene, The mortality, reduced ratio of weight, ecdysis index and schedule time of every instar.

本文对转Bt C肽蜘蛛杀虫肽的白桦进行杀虫性测定,对取食对照和转基因植株舞毒蛾幼虫的死亡率、体重降低率、蜕皮指数、幼虫各龄级历期等指标进行了分析,证明外源基因已经成功插入白桦基因组中,并且能够表达杀虫蛋白。

To test the toxicity of spore-crystal formulation of HBF-1 specific to larvae of several main scarabaeid species in North China, Bacillus thuringiensis suspensions mixed with sterilized fine soil containing potato pieces were used for feeding the larvae. The LC50 of HBF-1 crystal-spore powder to Popillia quadriguttata, Anomala exoleta, A. mongolica, A.

以我国筛选出的对金龟科幼虫具有杀虫活性的HBF-1菌株为供试菌株,采用菌悬液与含有土豆丝的土壤混合饲喂幼虫的方法,测定了HBF-1菌株孢晶粉剂对华北地区主要金龟子幼虫的毒力,其敏感性依次为四纹丽金龟Popillia quadriguttata,黄褐丽金龟Anomala exoleta,蒙古丽金龟A.mongolica,铜绿丽金龟A.corpulenta和苹毛丽金龟Proagopertha lucidula,LC50为3.104,18.987,30.924,34.713和55.995 μg/g土。

Result In direct effect experiment, 4.5% high active cypermethrin emulsifiable solution had strongest toxic effect on Coccinella septempunctata larva with the mortality of 93.33%; 20% haoniandong emulsifiable solution had strongest toxic effect on Coccinella septempunctata adults with the mortality of 56.67%.

结果]直接作用试验结果,以4.5%高效氯氰菊酯乳油对七星瓢虫幼虫的毒杀作用最大,死亡率达到93.33%; 20%好年冬乳油对成虫的毒杀作用最大,死亡率达到56.67%。

However, the Cry1Ac-GFP fusion protein only accreted with peritrophic membrane of resistant strain and showed fluorescence, which suggested that the binding of peritrophic membrane with Cry1Ac toxin protein may result in resistance of larvae of H. armigera to Cry1Ac insecticidal crystal proteins.

比较Cry1Ac杀虫蛋白敏感和抗性棉铃虫幼虫中肠的发光部位,敏感棉铃虫幼虫的中肠围食膜已经消失,肠壁细胞发出强烈的荧光,而抗性棉铃虫的围食膜较健全并发出荧光。

Foreign gene could destroy larvas mid-intestine, and reduce its appetite. Observation by microscope showed that the gaps between mid-intestine cells was extended in treated larvae, and the nucleus were destroyed, the gap between peritrophic membrane and mid-intestine cells was appeared, parts of peritrophic membrane was broken off from mid-intestine cells.

外源基因对舞毒蛾幼虫的杀虫机理在于破坏幼虫的中肠组织,影响幼虫的取食,使幼虫取食量减少,甚至终止取食;中肠细胞间隙增大,细胞核遭受破坏;围食膜与中肠细胞之间出现空隙,甚至脱落,围食膜不再完整;中肠组织脱落,成为一个严重变形的残架。

When transgenic rape leaves were fed to Plutella xylostera, mortality of second instar larvae ranged from 33% to 47% and the survival larvae had a less weight than the control.

对叶绿体转化植株进行虫试,结果表明,8天后小菜蛾幼虫的死亡率为33%-47%,未死亡的幼虫比对照体重减轻。说明所获得的叶绿体转基因植株具有杀虫和抑制幼虫生长的作用。

ResultsCyromazine had a great toxicity to firs t-instar larvae of housefly with LC 50 value of 43.896 mg/L, and showed less toxic actio n to third-instar larvae and adults of housefly, but reduced the pupate, ecl osion, female oviposition and egg hatch.

结果 灭蝇胺对家蝇 1日龄幼虫的 48h的LC5 0 值为 43 。896mg/L ,对 3日龄幼虫和成蝇的毒杀效果较差,但可引起幼虫化蛹率、羽化率以及雌成蝇产卵量和卵孵化率明显下降;阿维菌素对家蝇 1日龄、3日龄幼虫和成蝇表现出较强的急性毒杀作用,其 48hLC5 0 值分别为 0 。162 1mg/L、1.5546mg/L和 0 。2 10 3mg/L ,而对家蝇生长发育无不良影响。

Control effects of the seedlings, whorl leaves and the leaves of transgenic Bacillus thuringiensis corn on the 1 st instar, 4th instar and 5th instar larvae of Mythimna separata were evaluated in laboratory.

在室内用生测法研究了表达Cry1Ab杀虫蛋白的Bt玉米MON810和Bt11小苗和心叶对粘虫Mythimna separata初孵幼虫以及玉米叶片对暴食期4龄和5龄幼虫的杀虫效果。

Control effects of the seedlings,whorl leaves and the leaves of transgenic Bacillus thuringien- sis corn on the 1st instar,4th instar and 5th instar larvae of Mythimna separata were evaluated in laboratory.

在室内用生测法研究了表达Cry1Ab杀虫蛋白的Bt玉米MON810和Bt11小苗和心叶对粘虫Mythimna separata初孵幼虫以及玉米叶片对暴食期4龄和5龄幼虫的杀虫效果。

In SGK321 with expression of CpTI and Bt insecticidal protein genes, both petal and leaf showed significantly higher insect resistant activity than bud and boll heart.

结果表明:7月中旬两种转基因抗虫棉抗虫效果均较好,尤其是CpTI-Bt棉棉叶和花瓣对4龄幼虫3天内致死率为92%以上;8月中旬两种转基因棉的抗虫活性均明显降低,且Bt棉的杀虫活性显著低于CpTI-Bt棉,其幼虫死亡率与对照受体棉中16的死亡率之间无显著差异,仅显著抑制了幼虫的生长;石远321(SGK321受体品系)的花瓣具有一定的抗虫活性,可显著降低取食幼虫的体重,甚至造成部分幼虫死亡;CpTI-Bt棉中,花瓣和棉叶的抗虫性明显高于蕾和铃心。

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