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We observed changes of GV chromatin configurations in porcine, caprine and bovine oocytes from follicles of different sizes and degrees of atresia and during maturation in vivo and in vitro, using a new method for preparation of GV samples.

本项目发明了一种特殊的卵母细胞生发泡标本制备技术,能克服脂肪滴等对生发泡染色质构型观察的阻碍;经Hoechst或CMA3染色后可清楚地观察染色质构型的变化。

The class as a regular classroom management system, regulating the behavior of students' classroom teaching and cultivate students' order, good study habits and in practical teaching practice plays an important role. In 2001, China has a new foundation education curriculum reform,"born after the tide for the concept of" new order of teaching the "call" is formed on the traditional classroom teachers "speech" and monologic students listen up the strong shock status.

课堂常规作为课堂中的一项管理制度,规范着学生的课堂行为和课堂教学秩序,培育着学生良好的学习习惯,在具体教学实践中起着重要的作用。2001年我国掀起了新基础教育课程改革的浪潮后,&以生为本&的理念对教学新秩序的&召唤&己经构成了对传统的课堂中教师&独白式演讲&和学生们的端坐静听现状的有力冲击。

In Vajra dorje guru mahatma 尕 nan rinpoche's home to earnestly hope bear sons protect wheel, mahatma 尕 nan rinpoche to use Yidam's ShengleSutras practicing the Dharma to blessing the bear sons protect wheel,on the protect wheel, cover two mudras , granted and predicted his maternal uncle:please always wear it on your sister 's body, it available to all of the meritorious two brothers very soon.

仁波切家里原本只有三个女孩,仁波切的舅舅西然彭措为给妹妹求子,在金刚上师大圣尕南仁波切处祈请生男护轮,大圣尕南仁波切使用本尊胜乐经修法加持生男护轮,在护轮上盖了两个手印,赐予西然彭措舅舅并预言:此护轮让你妹妹常带身上,不久可得对众生有功的兄弟俩。

However, when two maturing oocytes fused and two spindle will form in the big cell, the chromosomes will not intermingle. In the present study, we removed GV from one oocyte and transfer to the perivetelline space of another GV oocyte. After fusion the resulting oocytes which contained two GVs were cultured further in MEM.

在本实验中,我们利用小鼠GV期卵母细胞将一枚卵母细胞的生发泡取出后移至另一未经去核处理的GV期卵母细胞透明带下,经三次电脉冲作用后将融合的含有两个GV的卵母细胞放入MEM成熟培养液中培养,在培养成熟的不同阶段分别收集卵母细胞进行免疫荧光染色观察微管及核的变化。

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