末端的

Three expression vectors containing mutant gfp which were truncated from 3′ termine were constructed in the present paper.The mutation gfp with different length under the control by T 7 promoter were expressed in E. coli BL21 cells.

本文将突变型 gfp3′末端不同程度地截短，构建成表达载体，在大肠杆菌 BL 2 1中表达了不同长度的绿色荧光蛋白，当其生色基因中 65位 Ser点突变成 Thr，则 GFP的激发波由 396nm增至 4 70 nm，不用 UV激发，仅在自然光源下就可看到强烈的绿色荧光，发光强度增加 6倍，若从 3′端截短 12个氨基酸密码，对发光无影响，但截短 75个氨基酸密码，则激发波长降到 360 nm，发光强度减少 12倍。

Objective: Mannose-binding lectin is a plasma protein belonging to the family of collectins, composed of a N-terminal segment, helical coiled-coil hinge region, collagen-like stalk domain., and globular type C lectin domain or carbohydrate recognition domain. Full MBL biological function requires assembly to at least the tetrameric level.

目的：甘露聚糖结合凝集素(Mannose-binding lectin， MBL)是属于C型凝集素家庭胶原凝集素的一种血清蛋白，包括N末端区（N-terminal segment）、螺旋铰链区（helical coiled-coil hinge region）、胶原样区（collagen-like region，CRL）及球形C型凝集素区或糖识别区（carbohydrate recognition domain ，CRD）等4部分结构区域，四个亚单位的MBL形成有功能的多聚体。

That of partial dissociated phycobilisomes indicated three bands: F652, F660 and F685, characteristic of C-phycocyanin, allophycocyainn and torminal emitters respectively.

部分解离藻胆体的荧光光谱的主峰位移至652nm：次峰位于685nm；660nm为一弱荧光发射肩。它们依次为C－藻蓝蛋白，末端发射体和别藻蓝蛋白的荧光。

Dixon transamination of oligopeptides and insulin results in the formation of a fluorescent derivative having emission maximum about 398-408nm with 0.02-0.03 quantum yield by the excitation at 3l2-333nm depending on the nature and length of the peptide.

短肽及胰岛素的N-末端经Dixpn转氨后具有荧光发色性质，其激发与发射波长随肽链氨基酸残基的种类和数量的不同而有差异，其范围大约为Ex 312—333nm；Em 398—408nm；量子产率为0.020—0.03。

However, there's no effective measures put in practice to evaluate and improve the sustainability of the industrial parks because of our unadvanced environmental management technology.

然而，我国工业园的环境管理手段还相对落后，偏重于末端治理，缺乏对工业园的系统评价和可持续性的有效提高。

A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物，自接种H5N1亚型病毒的鸡胚组织中提取RNA，反转录后采用高可信度DNA聚合酶经PCR扩增M1基因，采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达，纯化获得N末端携带多聚组氨酸标签的重组蛋白，分子量约29.8 kDa。

The course of the "Roman Wall" can also be traced eastwards to the Segedunum Roman fort in Wallsend - the wall's end and to the supply fort Arbeia in South Shields.

向东直到沃尔森德的Segedunum罗马城堡——长城末端，和南希尔兹的Arbeia可以发现&罗马城墙&的遗址。

In this paper,thetype of flow in perforated pipe was analysed,and the influence of which on calculating waterhead losses was simulated by computer programme.

大量计算表明，灌溉中使用的小流量多孔管的首端流态一般是紊流光滑区和紊流过渡区，而在末端或多或少出现一定长度的层流段。

We also show that BH1 domain is essential for Mcl-1 to inhibit Puma-induced apoptosis, since Mcl-1 mutant lacking BH1 domain completely abrogates its protective function.

最后我们发现除了PEST和BH1结构域介导Mcl-1的快速降解之外，还存在其它的降解信号，而且位于Mcl-1蛋白的C末端。

In the fixation of a tendon to bone the apposing surfaces of both bone and tendon should be scarified to hasten attachment.

最简单的肌腱固定方法需要按第63章描述的方法之一即在肌腱末端缝置一针缝线，借助于此缝线将肌腰牵向远端，使其拉直，确定肌腱的固定点。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失