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Rapid amplification of cDNA end:Retroverse transcription product of total RNA extracted from normal porcine tissue was used as the template,gene specific primers were designed and advantage 2 polymerase mix was used in PCR,of which using porcine genomic DNA as the template:forward primer was designed according to the acquired consensus region of human and pig FGL2 3′ sequences while reverse primer was designed from human FGL2 3′ end downstream sequence;TA cloning.

以猪正常小肠及心脏组织提取新鲜总RNA,反转录后作为模板,设计基因特异性引物,采用Advantage 2 聚合酶混合物进行PCR扩增;依据猪与人FGL2基因3′端已知同源序列设计PCR上游引物,以人FGL2基因3′末端序列设计下游引物,以猪基因组DNA为模板采用Advantage 2 聚合酶混合物进行PCR反应;PCR载体重组质粒DNA亚克隆扩增。

RhaG hydrolyzed only the 6-C α-rhamnoside of ginsenoside Re, RhaR is able to hydrolyze the α-rhamnoside of rutin、hesperidin and naringin.

实验结果表明,RhaG只水解人参皂苷Re的C-6位末端上的一个α-鼠李糖基,而RhaR能水解芦丁、橙皮苷、柚皮苷的α-鼠李糖基。

The roentgenological manifestations were mucosal fold changes, filling defects, luminal stenosis, changes of cecum and terminal ileum involvement etc.

该部位病变最常见的X线征象有粘膜皱襞改变、充盈缺损、肠腔狭窄、盲肠改变并累及末端迴肠等表现。

In one preferred embodiment, the applicator is a once usable tube containing a single dosage of formulation, and has an aperture, preferably equipped with a rupturable removable cap, provided at the distal end thereof, i.e. the end to be disposed adjacent to the patient's cervix on full and proper insertion into the vagina.

在一个较优的实施例中,该给药器为一个含有单次剂量药物的一次性塑料管,末端(即完全、恰当地插入阴道后,毗邻患者宫颈的那一端)有一个孔眼,在孔眼上安装一个可断开除去的盖帽更佳。

Herbs perennial, essentially glabrous (scabrescent around nodes and at ends of peduncles and rays).

多年生草本,基本无毛(在节周围和在的花序梗和伞辐末端稀疏毛)。

Seeds with scalelike membranous appendages at each end.

在每个末端的种子具鳞片状的膜质附属物。

"Schiffli lace, a type of embroidery, is made by modern machines, evolved from a hand version, using needle s with points at each end."

席福立花边为刺绣花边的一种,用现代缝纫机器缝制而成,从手动版逐步演变而来,用针线的点状织法缝制在各个末端

"Schiffli lace, a type of embroidery, is made by modern machines, evolve d from a hand version, using needles with points at each end."

席福立花边为刺绣花边的一种,用现代缝纫机器缝制而成,从手动版逐步演变而来,用针线的点状织法缝制在各个末端

"Schiffli lace, a type of embroidery, is made by modern machines, evolved from a hand version, using needles with points at each end."

席福立花边为刺绣花边的一种,用现代缝纫机器缝制而成,从手动版逐步演变而来,用针线的点状织法缝制在各个末端

In Wistar rats, latent period of a-wave in scotopic rod response after 1-week transplantation was shorten (P.05); and latent period of a-wave in scotopic rod response after 2-week transplantation was prolonged (P.05); while latent period of b-wave in scotopic maximum response after 1-week transplantation was shorten (P.05). In RCS rats, amplitude of a-wave in scotopic rod response and photopic response after 4-week transplantation was increased (PO.05); while latent period of a-wave in scotopic rod response after 4-week transplantation was shorten (PO.05).Conclusion 1. At the age of 3 months, photoreceptors of the RCS rat degenerated completely, which leaded to the reduction of amplitudes of a-wave and b-wave.

Wstar大鼠术后1周的暗适应视杆反应,a波潜伏期缩短o叼。05);术后2周的暗适应视杆反应,a波潜伏期延长o功刀5):术后1周的暗适应最大反应,b波潜伏期缩短o功刀5卜*CS大鼠术后4周的暗适应视杆反应和明适应反应,a波振幅增大o功刀匀;术后4周的暗适应视杆反应,a波潜伏期缩短o<0卜结论1、RCS大鼠在3月龄时感光细胞变性死亡,导致FERG的a、b波振幅明显降低;视网膜下腔仍存在感光细胞外节盘膜碎屑,外丛状层缺乏感光细胞的突触末端,同时Muler细胞出现反应性胶质纤维增生。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。