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Based on the fact that noΔM,ΔMM orΔGM fractions were separated,we postulated that the hydrolysis mainly occurred between two guluronic acids making one guluronic acid residue on the reducing end and an unsaturated guluronic acid in the nonreducing end.

根据以上实验结果推测裂解位点位于两个古罗糖醛酸之间,使得一个古罗糖醛酸变成不饱和糖醛酸位于产物的非还原末端,而另一个古罗糖醛酸位于产物的还原末端

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周,炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节,另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg,20分钟后踝关节照光,激光波长627.sum,功率密度 100mwcm',照射时间1000秒,能量密度100)/。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径,治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后,治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗,随机治疗一侧膝关节,另一侧作自身对照。兔耳静脉注入I'arrainrelomg/Kg,20分钟后,膝关节用金蒸气激光照射,激光能量密度100)儿旷。24 /J'时后取膝关节滑膜作病理检查,并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果: 1。模型观察:CIA大鼠炎症高峰期滑膜下炎细胞浸润明显,滑膜细胞明显增殖,炎症达到高峰后二周,血管缀形成,并侵蚀和破坏软骨和骨, CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生,滑膜下炎细胞浸润,也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明,各组织对HMME 的吸收速度和吸收量不同,荧光值一时间曲线不同,滑膜组织比皮肤和软骨对 HMME的吸收多,在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明:PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性,但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好,统计学检验差异有显著性。。3_军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少,图像分析结果表明面密度(阳性染色的面积总和与统计视野面积的比值)治疗组小于对照组,统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多,图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组,统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小,与对照组相比,统计学检验差异有显著性。结论:二。CIA、AIA的病理改变类似人类RA,可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同,滑膜组织比皮。

The lift unit includes a nacelle and a tailboom, and pivotally couples to the first end of the suspension structure, and a payload unit couples to the structure's second end.

空运装置包括引擎机舱和尾杆,枢轴耦合于悬挂结构的第一末端,且有效载荷装置耦合到所述悬挂结构的第二末端

The following results have been obtained: 1 Expression of SbNHX1 in E. coli enhanced Na+ and K+ tolerances, but not to others; 2 The sequence from 1353-1514bp in SbNHX1 gene played a role for SbNHX1 to Na+ and K+ tolerances; 3 The Na+/H+ antiporter of Salicornia Bigelovii Torr. catalysed Na+/H+ or K+/H+ exchange with equal affinity, which indicates that the SbNHX1 is more likely to locate on member of vacuolar, and responds to transport Na+ into the vacuole from cytoplasm.

证明了SbNHX1能抵抗Na+和K+的胁迫,但对其它盐离子没有抗性;2)证明了北美海蓬子Na+/H+逆向转运蛋白基因(SbNHX1)C末端从基因的C末端1353bp至1514bp的序列在耐盐中起一定的作用;3)证明了北美海蓬子Na+/H+逆向转运蛋白(SbNHX1)对Na+和K+交换的等亲和性,对于这一点,说明北美海蓬子Na+/H+逆向转运蛋白与定位于液泡膜上的Na+/H+逆向转运蛋白的功能相似。

The packing arrangements in their mesophases of the polymers were influenced by the end functional group obviously. All the polymers with amide-substituted group showed the partly interdigitated bilayer smectic phase structure.

另外,聚合物的热行为和相转变行为受液晶聚合物的末端键接基团影响显著,所有末端包含酰胺基团的侧链偶氮苯聚合物在液晶温度均形成了部分穿插的双层近晶相结构。

Methods The model of subacutely aging rats were made by injecting Dgal into the rats, abdominal cavity continually. After lavaging HSWY, the apoptosis rate and the levels of Fas and FasL mRNA were measured by TUNEL and RTPCR; the expressions of Fas/Fasl protein were detected by SP immunocytochemistry.

采用D半乳糖连续腹腔注射致亚急性衰老大鼠模型,同时以何首乌饮灌胃作为延缓衰老组;造模后应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法检测各组大鼠卵巢细胞凋亡情况;测定各组Fas和FasL的信使核糖核酸水平;SP免疫组化法检测卵巢细胞Fas和FasL蛋白的表达情况。

A series of UV curable waterborne hyperbranched polyester s with different acid/amine terminal rate,which contained -OH?-COOH and methacrylic chain end groups,and were partly neutralized with thertiary amine,were synthesized from trimellitic anhydride,epichlorohydrin,glycidyl methacrylate and triethylamine.

以偏苯三酸酐、环氧氯丙烷、甲基丙烯酸缩水甘油酯、三乙胺合成了末端羧基与氨基不同比例的一系列可UV固化的高支化聚酯,研究了末端官能团对合成产物水溶性、粘度以及凝胶转化率的影响。

All specimens are of small size, mostly less than 150 μm in diameter. In gross morphology, those problematical microfossils consist of stout tubes with closed base, tiny internal mold of elongated tube, parallel clustered unbranching tubes, spicule-like structure with expanded circular base, and branching tubes of various bifurcating styles.

作者在处理陕南末元古纪的化石过程中,发现了大量不同形态的微体管状化石(直径大多小于150μm),包括末端封闭的粗短管体,不分枝管体,末端扩展的骨针状构造及具多种分叉样式的分枝管体。

In addition, because the trajectory error of the end effector is increasing when the joints are motivated by untuned joint signals, a tuning method of joints is proposed to reduce the trajectory error. The effectiveness of this method is proved in the experiment.

另外,为解决实验中发现的机器人末端轨迹误差较大的问题,根据实验特点设计了机器人关节角输入补偿策略,提高了实验中机器人末端轨迹的跟踪精度。

Results One ureterostoma leaked when the water volume reached 135 cm,1 leaked when it reached the 167 cm, 2 leaked when it reached 175 cm, and others didn't leak when it reached 197 cm.

结果 本组25例检测标本中,在水柱高度达到135 cm、67 cm、75 cm时输尿管末端开始出现漏水者各为1例、1例、2例,另外21例标本在水柱到达197 cm时输尿管末端仍没有出现漏水现象。

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