未表达的

The expression of RACK1 was conserved in various human organs and did not display a robust and stable pattern of rhythmicity in mouse brain. Up and down regulating of Per1 had no significant effects on the RACK1 expression in cell tests.

结果 人下丘脑区域RACK1蛋白与Per1存在相互作用；RACK1在多器官组织保守表达，但其表达未呈现明显节律性；上调及下调Per1表达，RACK1的RNA水平无显著变化。

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

Results:In the endometrial carcinoma tela,the lack of expression of protein pten existed in endometrioid adenocarcinoma mainly,which accounted for 60 per cent.

结果：子宫内膜癌组织中pten蛋白的失表达主要局限于子宫内膜样腺癌，占60%，5例浆液性癌中则未见pten的失表达，pten失表达在这两组肿瘤间的差异有显著性(P=0.006)，p53蛋白过表达在子宫内膜样腺癌中占26.7%，在浆液性癌中为80%，两组间差异有极显著性(P=0.001)。

A portion of ductal cells and individual serous acinic cells of the minor salivary gland also expressed Fas not only in membranous but also in cytoplasmic pattern.

结果发现，在正常鼻咽粘膜表面及隐窝的假复层纤毛柱状上皮细胞胞膜、部分小涎腺的导管上皮和个别浆液性腺泡上皮细胞的胞膜和胞浆Fas阳性表达；鳞状化生上皮的表层细胞胞膜有Fas表达，但基底细胞未见Fas表达；浸润的淋巴细胞也不表达Fas.45.9%（17/37）的鼻咽非角化性癌Fas阳性，但只有不足10%的癌细胞胞膜微弱表达Fas。

Results Although overexpression of P53 protein was not detected in normal eyelid cutaneous epithelium, meibomian gland and mild epithelial dysplasia, the positive rate of P53 increased in degree in moderate and severe dysplasia, carcinoma in situ and invasive tumors, and PCNA labeling index progressively increased too. Overexpression of P53 protein was detected in 30/53 cases of basal cell carcinoma, 16/32 cases of squamous cell carcinoma, 10/17 cases of meibomian gland carcinoma, and the intensity of P53 protein immunostaining increased with the lowering in the degree of histological differentiation.

结果 在正常眼睑皮肤、睑板腺及轻度不典型增生眼睑上皮中，未见P53蛋白表达，而中、重度不典型增生，原位癌，浸润癌中，P53蛋白阳性表达率逐渐增高，其 PCNA指数也有增加趋势。30/53例基底细胞癌(basal cell carcinoma，BCC)、16/32例鳞状细胞癌（squamous cell carcinoma，SCC）、10/17例睑板腺癌中，P53蛋白阳性表达；同时，随着肿瘤组织分化程度的降低，P53蛋白阳性表达程度有逐渐增高的趋势。

Results Although overexpression of P53 protein was not detected in normal eyelid cutaneous epithelium, meibomian gland and mild epithelial dysplasia, the positive rate of P53 increased in degree in moderate and severe dysplasia, carcinoma in situ and invasive tumors, and PCNA labeling index progressively increased too.

结果 在正常眼睑皮肤、睑板腺及轻度不典型增生眼睑上皮中，未见 P53蛋白表达，而中、重度不典型增生，原位癌，浸润癌中， P53蛋白阳性表达率逐渐增高，其 PCNA指数也有增加趋势。30/53例基底细胞癌(basal cell carcinoma，BCC)、16/32例鳞状细胞癌（squamous cell carcinoma，SCC）、10/17例睑板腺癌中， P53蛋白阳性表达；同时，随着肿瘤组织分化程度的降低， P53蛋白阳性表达程度有逐渐增高的趋势。

Results The HSP70 expression markedly increased after 4h of depilating treatment, and distinctly decreased after 16h of depilating treatment. The HSP70 expression had no significant difference between the GV and its bilateral sides.

结果 脱毛后恢复期4h时皮肤高度表达HSP70恢复期16h时HSP70表达减少；督脉线与其两侧旁开非经对照点的HSP70表达在恢复期4h和16h时均未见明显差异。

Under the transmission electron microscope, we found adjacent cells in rat MSCs with mHCN4 gene and neonata atrial cells in coculture often had intimately apposed interdigitating cell membranes.

经检测mHCN4基因修饰大鼠MSCs可表达Cx43，其表达水平明显较GFP组及未转染组高，与心房肌细胞的Cx43表达水平无明显差异。

The NPC tissue was all EBV-positive except for one sample, which was identified as keratinizing carcinoma, but the control samples were all negative for EBV infection, which was correlated with CD25 expression.

对照组选取鼻咽慢性炎症组织。结果 CD25淋巴细胞在鼻咽癌组织中比在鼻咽慢性炎症组织中的表达明显升高，差异具有显著性意义(P.001)，且在未分化癌中表达高于角化型癌和非角化型癌(P.05)。原位杂交显示EB病毒与CD25淋巴细胞高表达有关。

Flounder FoxD1 mRNA was over expressed in one cell of the two-cell stage zebrafish, MyoD expression in the somites on one side was reduced, but the expression in the adaxial cells was not affected; Myf5 expression in the presomitic mesoderm, adaxial cells and somites on one side was reduced.

在斑马鱼中过量表达FoxD1后，MyoD在一侧体节中的表达受到了严重抑制，而在近轴细胞中的表达未受影响，Myf5在体节前中胚层，近轴细胞，及体节中的表达都受到了抑制。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。