未表达的

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Results:(1)Among 40 rats, 36 rats were successfully established and the rate of success is 90 percent;(2)All the successfully established models demonstrated polydipsia, polyuria and the body weight was not increased. 6 rats suffered cataract after 3 months, and 4 rats died in 6 months;(3)There was an approximately 61% loss of retinal ganglion cells in the central retina and the thickness of retina thinner under microscope ( P 0.01 ).(4) Electroscope changes include the thinner of retina, disorganization of the membranous disc of the rod cells and the thickness of basal membrane of vessal.(5)In normal group, 1 month dibetes mellitus and 1 month treatmen group, there was no expression of ERK1/ERK2 on the retina tissues. In 3 month diabetes mellitus group, the expression of ERK1/ERK2 was positive.

结果：①40只建模大鼠中36只建模成功，建模成功率为90%；②建模成功的大鼠都表现出多饮、多尿、消瘦、体重不增的表现，有6只大鼠在3个月后出现白内障，有4只大鼠在喂养接近至6个月时死亡；③HE染色光镜下6个月大鼠后极部视网膜节细胞层细胞数明显减少，减少约61%(P.01)，后极部视网膜明显变薄(P.01)；④电镜观察，视网膜变薄，视杆细胞膜盘紊乱，血管基底膜增厚等表现；⑤正常组、糖尿病组和治疗组1个月大鼠视网膜中未见ERK1/ERK2的表达，糖尿病组视网膜组织中3个月时可见少量表达，ERK1/ERK2表达部位为神经节细胞层和内核层；6个月时表达强阳性，部位表达不仅见于内核层、神经节细胞层，色素上皮层也见表达。

LPL was expressed in many tissues in tree shrew, highly in cardiac muscle and adipose tissue. In despite of no expression in skeletal muscle, there was less-medium expression in liver, distinctly different from human LPL which was expressed in the contrary way in these two tissues.

树鼩脂蛋白脂肪酶的组织表达谱比较广泛，在心肌和脂肪组织中大量表达，但在骨骼肌中未检测到，却在肝脏中有中低水平表达，与人脂蛋白脂肪酶在这两种组织中的表达相反。

Among the 3 novel sequences, C24 sequence and C57 sequence were expressed both in cataractous group and in normal group; C24 sequence was expressed higher in cataractous group than that in the normal group, but C57 sequence had no significant difference in normal and cataractous group; C40 sequence was expressed in the cataractous lens epithelium but the expression in normal lens epithelium was not seen.

三个新序列中，C24号和C57号序列在年龄相关性白内障和正常晶状体上皮细胞中均有表达，C24号序列的表达增高；C57号序列在正常组和白内障组中的表达无差异。C40号序列在年龄相关性白内障晶状体上皮细胞中有表达，但其在正常晶状体上皮细胞中未见表达。

Results MMP-2 positive cells are localized in peripheral columnar while no positive cells are seen in odontogenic karatocysts and dentigerous cysts.

结果 成釉细胞瘤中MMP-2阳性染色位于肿瘤外周柱状细胞的胞浆中，中心星网状细胞未见表达；角化囊肿和含牙囊肿的上皮细胞中均未见MMP-2的阳性表达。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

In situ hybridization was used to observe the expression of the MCP-1 mRNA in the walls of the normal arteries and the aneurysms. Results: by the HE straining the walls of the ruptured aneurysms (10 cases) and unruptured ones (2 cases) were consisted of incrassated intima and connectivum extima.

免疫组化：MCP-1组：正常颅内动脉未见MCP-1阳性细胞，未破裂动脉瘤和破裂动脉瘤的阴性对照未见染色细胞。2例未破裂和10例破裂动脉瘤：MCP-1呈高表达，阳性细胞在动脉瘤壁内呈局灶聚集，间断分布动脉瘤内膜。

FGFR4 expression was faint in renal vesicle and primitive tubules of S-shaped body, irrecognizable in urteric bud and podocyte of C-stage, negative in mesenchyme and condensing mesenchyme.

胚胎肾发生带内FGFR4表达微弱，肾囊泡和S型小体的上支和中间支，即原始肾小管上皮细胞部位有微弱表达，间充质、压缩间充质未见表达，输尿管芽及其末端壶腹和C-期足细胞表达不明显。

The expression of subunits α5,β1,β3 was detected in normal morulas and increased in blastocysts. The expression of subunit α1 was not detected in morulas or blastocysts, but detected only in outgrowing trophocytes. 3. Expression of integrin β3 in irradiated group embryos was weaker than that of normal group. There was no expression of integrin α1 both in morulas and blastocysts in irradiated group and no difference in the expression of subunits α5 and β1 between these two groups.

结果：1、超声波照射组胚胎贴附率、滋养细胞外延生长率均显著低于正常胚胎。2、正常胚胎桑椹胚期即有α5、β1、β3整合素表达，而无α1表达；囊胚期α5、β1、β3表达增强，仍无α1表达；α1只出现在外延生长的滋养细胞。3、超声波照射组胚胎β3整合素的表达明显弱于正常组，且未见α1表达；而α5、β1的表达两组间无差异。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。