未表达的

The results showed the bacterial supernatant and periplasm containing NKAT2 scFv were able to bind the NKAT2 receptors expressed on cell surface,but NKAT4 scFv failed to bind to NKAT4 receptors.

结果显示，含NKAT2－scFv的细菌上清及胞质提取物均可识别到表达在细胞表面的NKAT2受体分子，但含NKAT4－scFv的细菌上清及胞质提取物均未成功

The prolif- erative ability of CD133 + tumor cells in vitro was estimated by MTT assay, and the proliferative ability of CD133 +, CD133 - and control SUNE cells were statistically compared. Finally, the immunocytochemical staining method and flow cytometry were used to detect differen- tial ability of CD133 + tumor cells in vitro .

采用免疫荧光细胞化学技术及流式细胞仪检测鼻咽癌细胞株SUNE中CD133的表达情况以及CD133+细胞体外分化能力；免疫磁珠分选技术纯化CD133+肿瘤细胞；MTT法检测CD133+细胞的体外增殖能力，并将其与CD133-及未分选细胞进行比较。

The low expression of BMP5 mRNA in undifferent mesenchymal cells may contribute to pathogenesis of cleft palate.

BMP5 mRNA在未分化间充质细胞中的弱表达与腭裂的形成密切相关。

Jindao 1. The results showed that on the 2-DE gels stained by coomassie brilliant blue,PDQuest image software detected about 215、201、187、162、132 protein spots, of which 22 spots show more than 2.5-fold changes in abundance among ungerminated seed and embryo buds of germinated seed at specific stages of 1 d、2d、3d、4d in riceOryza sativa L.

发现金稻1号水稻种子未萌发和萌发1-4d胚在PDQuest图像分析软件可识别的蛋白质点约215、201、187、162、132个，其中表达量变化2.5倍以上的蛋白质点有22个。

Most of EGFR in unmatured and matured oocytes was in variation, and there was obviously more expression of variation in matured oocytes than that in unmatured oocytes.

未成熟和成熟的卵母细胞中主要存在Ⅷ突变型，而野生型表达相对较少，成熟后卵母细胞中的Ⅷ突变型蛋白明显增加。

PCR, Southern hybridization and SOD activity assay displayed that exogenous gene was integrated in five maize genomes, exogenous protein was over-expressed in partial plants and the activity of MnSOD of the transformed plants is more than 3 times of the untransformed control.

PCR、Southern杂交和SOD酶活性电泳检测表明，5个玉米基因组中已整合外源基因，部分转基因植株过量表达了外源蛋白，转基因株系中的MnSOD酶活性是未转基因对照植株的3倍以上。

RT-PCR was used to detect the expression level of receptor mRNA of follicle stimulating hormone and luteinizing hormone in sexually immature hens.

应用反转录多聚酶链式反应方法，检测镉中毒的性未成熟鸡卵巢卵泡刺激素受体和黄体生成素受体 mRNA表达水平的变化。

This result is not same as the reported one (one NADP-ME band was detectedin C3 plant). NADP-ME activities increased greatly under NaCl, NaHCO3, Na2CO3, PEG stresses.

然而，水稻质体型NADP-ME_1(C_3植物质体型NADP-ME)基因的表达却受光的诱导，这些结果未见报道。

Methods Fourteen mutated vpr fragments were selected from patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products were purified, double-cut by Hind Ⅲ and BamH Ⅰ, and the cut products were legated and transformed into competent cells JM109. The 14 reconstructed plasmids were transfected into Hela cells. Cells with pcDNA vpr-wt, pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell were established.

以14个带有HIV-1vpr基因片段的PcD-NA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vpr基因转染细胞、突变株vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经逆转录多聚酶链式反应检测目的基因转染成功后，Pi染色，用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Methods 14 mutanted vpr fragments selected from Chines patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products purified , double-cut by HindⅢ and BamH and the cut products legated and were into competent cells JM109. The 14 reconstructed plasmids electronically transfected into hela cells, and established cells with pcDNA vprwt 、pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell.

将14个带有HIV-1 vpr基因片段的pcDNA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vp r基因转染细胞、突变株 vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经RT-PCR检测目的基因转染成功后，经Pi染色用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。