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In Group II, 89% of sacs expressed GFAP by 7 compared to 79% in the contralateral undisturbed DRG.

第2组中7天时有89%的标本表达GFAP,在对侧未处理的DRG中有79%表达。

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性,我们利用原位杂交和免疫组化等方法,研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动,结果表明:(1)热对各阶段的雄性生殖细胞都有损害,粗线期的初级精母细胞尤为敏感,实验性隐睾内的精原细胞尚未完全受到"致命"影响,说明精原细胞对热有一定的耐受性,但即使成为粗线期/双线期初级精母细胞,却未能获得由有丝分裂过渡到减数分裂的能力,呈现不同程度的凋亡,所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞,更谈不上长形精子细胞和精子的形成;(2)腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录,说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的;(3)正常兔睾丸组织中,〓在精原细胞和粗线期/双线期精母细胞中均有表达:cyclin B1蛋白在减数分裂前期的粗线期/双线期初级精母细胞中的表达量增加,于变态末期的精子细胞中达高峰。

2The permillage of the positive Cardiomyocytes of the expression of Bax protein began to increase at 1h group and achieved the peak at 4h and then dropped gradually at 12h.

2免疫组化检测阳性表达的Bax蛋白积聚于胞浆内;心肌细胞Bax蛋白表达阳性的细胞千分率在冠脉结扎后1h已开始逐渐升高,4h时达高峰,然后逐渐下降,到12h已降至冠脉未结扎水平。

With western blot analysis and DNA polymerase chain reaction-single strand conformation polymorphism assay,protein product of Rb gene and mutation in exon 5~8 of P_(53) gene were examined in 17 cases of human testicular seminoma.

采用Western印迹和DNA聚合酶链反应-单股构造多态性分析法对17例人睾丸精原细胞瘤组织标本的抗癌基因Rb表达的蛋白产物和P_(53)基因外显子5~8的突变进行检测,发现有3例标本的Rb基因表达的蛋白产物缺失,有4例标本的P_(53)基因有点突变,对照的正常人睾丸组织和视网膜组织均呈Rb基因蛋白产物表达阳性,对照的正常人睾丸组织中未发现P_(53)基因突变。

OCT4 stained 1 unclassifiable tumor, which lacked other specific markers.

那3例未分类的肿瘤中有1例表达OCT4,但不表达其他特异标记。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上,选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型,进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制,探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制;以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制;以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响,从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

Objective To investigate estrogen and andro gen on the regulation of gene expression of insulin-like growth factorⅠmRNA in prostatic tissues. Methods IGF-ⅠmRNA was quantitat ively ass essed in BPH and SD rat prostatic tissues using dot blot hybridization technique .

目的 探讨雌、雄激素对列腺中胰岛素样生长因子Ⅰ基因表达的影响方法采用斑点杂交结合图像分析技术,观察药物去势和未药物去势BPH患者前列腺组织以及正常组、去势组、外源性雄激素组、雌激素组鼠前列腺组织中IGF-ⅠmRNA表达情况。

Results: The growth of NB4 cells was inhibited significantly after treated with berbamine at different concentrations for different time points, the IC50 value was 3.860μg/ml at 48 hours. Morphology analysis showed the characteristics of apoptosis, and the DNA agarose electrophoresis showed the typical DNA ladder. The apoptosis rate increased from 2.83% to 58.44% after treated with berbamine at 12μg/ml for 18 hours. The expression of PML-RARα mRNA presented no significant changes, however, Survivin mRNA was decreased dramatically.

结果:经不同浓度小檗胺处理不同时间后,NB4细胞生长出现明显的抑制,并呈现明显的时间剂量依赖性,48h IC50为3.860μg/ml;细胞形态学观察可见细胞凋亡现象,DNA琼脂糖电泳显示典型的凋亡梯形图:流式细胞仪检测发现细胞凋亡率明显增加,经48h作用后,凋亡率从2.83%增至12μg/ml时的58.44%;虽未发现药物作用后PML/RARα基因表达量的改变,但检测到NB4细胞经药物作用后Survivin基因表达量的减少和Caspase3表达的增加,Caspse3阳性率从对照组的2.06%增至12μg/ml时的70.89%。

Syn IR positive cells are with staining in cell plasm and without staining in nucleus.

CgA和Syn在类癌、小细胞未分化癌、大细胞未分化癌中阳性表达的符合率为58.8%。

With the methods of Reverse transcription-PCR and cDNA sequencing, the expression of FHIT gene was detected in radiation carcinogenesis. The results show that: 1, The FHIT gene's sequence in normal BALB/c mice compare with the sequence of mice in Ge neBank which absents exon 3, but the function of FHIT protien doesn't alter.

结果发现:1、正常BALB/c 小鼠的FHIT基因序列同GeneBank中小鼠的FHIT基因序列比较缺少了外显子 3,但其表达的蛋白功能并未发生改变;2、FHIT基因在辐射损伤与辐射致癌的早期过程中,对照组血液、胸腺及骨髓均未出现异常的FHIT转录本的表达,不同剂量照射组中血液、胸腺及骨髓均有部分样品出现分子量较小的异常FHIT 转录本的表达。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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