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CA II and AQP5 expression levels were determined by immunofluorescence and immunoblotting, while CA activity and Na, K-ATPase activity by a colorimetric assay. Anhidrotic mice treated by topiramate did not differ from controls in average secretory coil diameter, CA II expression, CA activity and Na, K-ATPase activity.

应用免疫荧光和western blot技术发现:小鼠在托吡酯处理后泌汗减少,碳酸酐酶同功酶II型(carbonic anhydraseII,CAII)蛋白表达未出现显著变化,但汗腺组织胞膜的水通道蛋白-5(aquaporin-5,AQP5)表达量下调。

Our findings are as follows: 1. Mice vestibular development initiated early and the process is short. All macula and crista ampullaris were formed at E15, which Myosin VI was expressed in hair cells at this time but not expressed in support cells.

结果发现:1。小鼠前庭的发育较早、过程较短,E15时所有囊斑、壶腹嵴基本形成,所有壶腹嵴、囊斑里均出现MyosinⅥ阳性表达毛细胞,未见支持细胞表达MyosinⅥ。2。

The results prove that CNHK500 has highly proliferation selectivity and potent cytolysis effect on breast cancer in vitro.

在感染CNHK500的端粒酶阴性的正常成纤维细胞株中未检测到E1A基因表达,在端粒酶阳性的感染CNHK500的乳腺癌细胞株中能够检测到E1A基因的表达;CNHK500可以选择性地在缺氧条件下表达E1B。

After thermal induction, no specific recombinant protein band in SDS-PAGE was found, but G-CSF activity was detectable. Therefore, a new recombinant plasmid pBVHG2 expressing hG-CSF hybrid protein with additional 8 amino acids which could be cut off specifically with the help of mucosal enterokinase at the N terminus of hG-CSF was constructed.

含此质粒菌株虽然表达菌体裂解后可测得明显的生物学活性,但SDS-PAGE仍未见特异表达产物带;因此,再应用相同方法,在hG-CSF cDNA突变体5′端增加24核苷酸对的FLAG肽编码序列,构建了hG-CSF杂交蛋白(hG-CSF天然蛋白N末端增加8aa的FLAG肽,后者可由肠激肽酶切除)的表达质粒pBVHG2。

Result The expression of TSHR was observed in the perimysium, cytomembrane and cytoplasm in 10 of the 12 TAO specimens, accounting for a TSHR expression rate of 83.3%in the EOM of TAO patients.

结果 TSHR在眼外肌肌束膜、细胞膜及少量胞浆中表达,12例TAO患者中有10例检测出TSHR蛋白,阳性检出率为83.3%,正常对照组未检测出TSHR蛋白的表达。

The results showed that UEA-1 receptors were mainly locolized on the surface of the glandular and luminal epithelium.

UEA-1受体在休情期犬子宫内未见表达,而在发情期犬子宫内膜腔上皮和腺上皮内的表达量很高。

Ovine immatured oocytes, matured oocytes and embryos from 2-cell stage to blastocyst produced in vitro were recovered, and RT-PCR was used to semi-quantify mRNA expression. Proteins of Cx43 and Cx45 were detected by using a combination of immunocytochemistry and Laser Scanning Confocal Microscope.

收集绵羊未成熟和成熟卵母细胞以及体外受精早期胚胎各发育阶段的卵裂球细胞,通过RT-PCR半定量检测Cx43和Cx45基因的mRNA表达量;通过免疫组织化学方法结合激光扫描共聚焦显微镜检测Cx43和Cx45蛋白在绵羊体外受精的早期胚胎发育过程中的表达情况及表达区域。

The protein had the same antigenicity as IgG, and the change of the protein had the same tendency as SHP-2, which was higher in canceration groups than in uncanceration groups and control groups in thymus cells, but there weren't significant change in bone marrow cells;⑧ The change law of the expression of SHP-1 and SHP-2 during γ-ray induced malignant transformation in BEAS-2B cell is that the expression of SHP-1 was increased synchronistically with malignant transformation of cell, and the expression of SHP-2 didn't have significant correlation with malignant transformation of cell.

癌变组胸腺细胞中SHP-2 mRNA编码PTPase催化区的700~1096bp间可能出现甲基化;⑦癌变组的胸腺细胞中存在一相对分子质量约为55×10〓的蛋白质,它与IgG具有相同的抗原性,其表达量的变化趋势与SHP-2的蛋白表达变化趋势基本一致,即在胸腺细胞中明显高于对照组及未癌变组,但在骨髓细胞中对照组高于癌变组;⑧SHP-1、SHP-2在γ射线诱发的BEAS-2B细胞恶性转化过程的变化规律:SHP-1蛋白表达量的升高与细胞的恶性转化在时间上基本一致,而SHP-2蛋白表达量与恶性转化无明显关系。

CgA IR positive cells are with staining in cell plasm and without staining in nucleus.

在类癌、小细胞未分化癌、大细胞未分化癌中的阳性表达率分别为54.5%、48.0%、44.4%。

Results:① Wnt7a was expressed in hEFCs and ICR mEFCs after culture normal karyotype hESCs, but not expressed in control and in hEFCs and ICR mEFCs after culturing abnormal karyotype hESCs with karyotypic change in chromosome NO.

Wnt9a的表达量在未培养正常hESCs的hEFs中表达量高,而在养hESCs后的hEFs中表达量低。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

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