未表达的

The results showed that TASL30 gene was expressed in significant amount in neural tube of early human embryo and the cephalic expression level was the highest,but none of recognizable positive signal was detected in 50 human tissues.

结果表明，TASL30基因在早期人胚神经管中有明显表达，其中在神经管的头端表达最强，而在人的50种器官组织中均未检测到该基因的明显表达。

It is shown from the detection results that VAMP-2 is expressed in intestine, liver, kidney, skin, spleen and sex gland as well as brain of Japanese sea perch at different levels, but no VAMP-2 is expressed in...

结果表明，除脑组织外，在肠、肝脏、肾脏、皮肤、脾脏、性腺等组织中也检测到 VAMP-2的表达，各组织间的表达水平亦存在差异，肌内组织未检测到表达。

Known differential expression gene: In the 46 points of known differential expression gene, there were down - regulate gene expression 41 points and up- regulate gene expression 5 points; twenty - eight genes related to hypoxia/reoxygenation had not been reported and 18 genes had been reported.

已知差异表达基因：在46个已知差异表达基因中，表达下调的基因有41个，表达上调的基因有5个，未见报道的与缺氧/复氧处理相关基因23个，已报道的有18个。

Methods Westernblot was employed to detect the intracellular expressions of caspase-9, hTERT, Bcl-2 and Bax, and mitochondrial and cytoplastic cytochrome C in HepG2 cells transfected by pSliencer 3.1-H1 neo-shTERT.

收集第20代hTERT基因RNAi真核表达载体论定转染细胞、对照细胞及未转染HepG2细饱，采用Western blot检测线粒体，胞质细胞色素C的表达及细胞内caspase-9、Bcl-2、Bax和hTERT的表达。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Suppression of metastatic hemangiosarcoma by a parvovirus MVMp vector transducing the CXCL10 chemokine into immunocompetent mice.

2.2 转染细胞表达CXCL10蛋白的检测采用Western blot分别检测转染CXCL10表达载体的细胞及其培养上清中CXCL10的表达，均可检测到CXCL10蛋白；而转染空载体组和未转染组细胞均未能检测到CXCL10存在

The proportions of Ⅰ~Ⅱgrade and Ⅲ grade were 73.8%、 83.1%; 45.7%、 65.2% between 92 samples of Urgur patients and 188 samples of han patients . Lymphy node metastasis in Uygur patients were greater than Han patients in Ⅰ~Ⅱgrade and Ⅲ grade (X~2=13.14, P.001; X~2=5.08, P.05).(3) The proportions of the expression of VEGF-AmRNA and its protein were 31.8%、 57.1%、64.3%, 27.3%、 53.6%、 59.5% in difference pathological grade in 92 samples, With increasing of the pathological grade,the VEGF-AmRNA and its protein expression are highly (X~2=6.26, P.05; X~2=6.21, P.05); The proportions of the positive expression of VEGF-AmRNA and its protein were 68.4

392例浸润性乳腺癌组织中VEGF-AmRNA和其蛋白的表达在不同组织学分级中其阳性率分别是31.8%、57.1%、64.3%，27.3%、53.6%、59.5%；二者在不同组织学分级中表达差异具有统计学意义(X~2=6.26，P.05；X~2=6.21，P.05)；在淋巴结转移组与非转移组中VEGF-AmRNA和其蛋白的表达阳性率的分别是68.4%、31.49，61.4%、31.4%，在淋巴结转移组VEGF-AmRNA和蛋白的明显高于淋巴结未转移组(X~2=11.96，P.001；X~2=7.79，P.05)；VEGF-AmRNA和其蛋白的表达

Indirect immunofluorescence was used to observe the distribution of fibronectin in human oocyte and 2-cell to morulae embryos.

纤维粘连蛋白均匀分布在体外未受精人卵母细胞和受精后人胚胎早期发育的不同阶段的卵裂球表面，各时期的免疫荧光强度未见明显差别。透明带未见纤维粘连蛋白表达。

Compare the 2-DE maps between IgA nephropathy and normal rat, 664 protein spots in 1 279 samples were matched, and 263 proteins were with over two-times high difference in the matched spots, 3 proteins were not expressed and 1 protein was uniquely expressed in IgA nephropathy.

比较IgA肾病和正常小鼠肾组织的2-DE图，在1 267个蛋白点中有664个匹配蛋白点，其中表达量差异在2倍以上的有263个，另有3种蛋白在IgA肾病肾组织中未被表达和1种蛋白特异表达。

The results indicated that four SOD bands were identified in five tissues, and the band with the biggest mobility ratio were found weakly in gill, digestive gland and adductor muscle but not in foot and mantle. Two ATPase bands were found in four tissues except digestive gland. One MDH band was found in five tissues, but the MDH gene expressed strongly in foot and adductor muscle. Two ME bands were examined in foot, adductor muscle and mantle, but the band with smaller mobility ratio was found in gill and digestive gland.

结果表明，SOD在紫贻贝的5种组织中均有表达，共有4条酶带，但迁移率最大的酶带在足和外套膜中未出现，而在另外3种组织中呈弱表达；ATPase在除消化腺外的组织中都表现为2条带；MDH在5种组织中都表达出1条酶带，其中足和闭壳肌的MDH染色最深；ME存在1~2条酶带，其中迁移率大的条带在5种组织中都有表达，而迁移率小的条带只存在于外套膜、闭壳肌和足中。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。