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The results show that the CD29, CD49, CD90 express in the gonium and the primary spermatogenous, but there are not expression in the oocyte, the second spermatogenous and the spermatocyte.

研究结果表明,CD29, CD49, CD90在雌性生殖腺各级卵母细胞中均不表达,位于生殖褶边缘的性原细胞中可见表达;在雄性生殖腺的初级精原细胞中表达,但在成团分布的次级精原细胞、各级精母细胞中均未表达。

The result showed that twocDNA fragments which expressed at high level both in shoot and radicle representedthe gene encoding beta-D-glucosidase; one cDNA fragment expressed specifically inshoot represented the gene encoding mitochondria HSP60; most clones of MF12 andMF17 fragments respectively represented the chloroplast genes encoding prp22 andprp19 proteins which are two components of ribosomal small subunit; while thededuced amino acid sequence from each exceptional one clone was respectivelyhomologous to CDC5 proteins and vesicle-associated membrane proteins; otherthree cDNA fragments expressed preferentially in shoot had no homologue inGenBank.

结果发现2个在胚芽和胚根中表达量都很高的cDNA片段代表的是编码玉米β-D-葡糖苷酶的基因;一个在胚芽中表达而在胚根中不表达的片段代表的是编码线粒体分子伴侣HSP60的基因;片段MFl2的大部分克隆测序结果是与叶绿体基因组中编码核糖体小亚基蛋白prp22的基因同源,但其中有一个克隆测定的cDNA片段序列推测的氨基酸序列与CDC5家族成员有较高的同源性;片段MF17的大部分克隆测序结果与叶绿体基因组中编码核糖体蛋白prp19的基因同源,而有一克隆测定的cDNA片段序列推测的氨基酸序列与参与信号转导的膜结合蛋白VAP27和VAMP有较高的同源性;另有3个优先在胚芽中表达的cDNA片段未查询到同源序列。

Humans often use their animals to express their unexpressed emotions.

人类经常用自己的动物去表达他们未表达的情绪。

Asymmetric over-introgression of the indica alleles on chromosome 7 and many specific regions on other chromosomes in the japonica genetic background is consistent with the general trend observed in the RIL and F_2 populations of the same cross, suggesting a high genetic load in the japonica gene pool, and japonica genome experienced a very severe bottleneck by artificial domestication.2 Genetic background effect on QTL expression of grain weight and its related traitsGenetic background effect on main-effect QTL and digenic epistatic QTL mapping for GWT and its related traits including GL, GW and GT was dissected by a large set of reciprocal introgression lines and recombinant inbred lines derived from Lemont and Teqing in Beijing environment.

籼粳相互导入具有不对称性,粳稻向籼稻背景的平均导入频率与期望值相仿,相反籼稻向粳稻背景的平均导入频率大大高于理论预期,位于第7染色体和一些其它染色体的特殊区段存在籼稻等位基因的超导入现象,在来自相同亲本的重组自交系和F_2群体中也观察到同样的导入趋势,表明粳稻基因组具有较大的遗传负荷,粳稻品种在人工驯化过程中曾经历过一个非常剧烈的遗传瓶颈。2水稻籽粒相关性状的主效和互作QTL表达的遗传背景效应利用Lemont和特青培育的双向导入系和重组自交系群体,在北京环境下定位影响千粒重及其相关性状如粒长、粒宽和粒厚的主效和互作QTL,分别在特青背景导入系、Lemont背景导入系和重组自交系下检测到影响千粒重及其相关性状的主效QTL分别为33个、27个和13个,互作QTL分别为4个、8个和23个,其中仅有6个主效QTL(QGwt5,QGl1,QGl3b,QGl4,QGw7和QGw8)在籼粳两个不同背景下共同表达,3个主效QTL(QGwt5,QGwt7a和QGt5)在导入系和重组自交系中共同表达,分别占检测到影响这些性状QTL总数的11.1%和4.3%,未检测到在3个背景下相同表达的互作QTL。

Observation with transmission electron microscope showed that interalveolar septa were widened with increased amount of collagen in the MP infected rats while there were no obvious abnormalities in the other two groups.(2) Positive expression of bFGF mRNA were found in alveolar walls of MP infected rats. No expression of bFGF mRNA was found in control animals. In the rats infected with MP but treated with erythromycin positive expression of bFGF mRNA was found to be scarcely distributed in alveolar walls.

2感染组动物肺泡壁见明显的bFGF mRNA阳性表达颗粒,对照组基本未见bFGF mRNA阳性表达,感染加红霉素治疗组仅见散在分布的少许bFGF mRNA阳性表达;定量分析结果表明,感染组bFGF mRNA阳性表达的积分吸光度为41.32±10.44,与对照组(0.30±0.13)和感染加红霉素治疗组(6.03±2.41)比较,差异有显著性( P <0.01)。

Though the molecular mass and the hypoglycemic activity of myristic acid modified HIDesB30 are both consistent with Detemir, the expression system, purification artwork and the methods of modification were different from Detemir.

本研究实现了含短C肽的人胰岛素原类似物HMPIDesB30在毕赤酵母中的高效分泌表达,表达量达1 g/L,分泌表达的重组蛋白HMPIDesB30未出现糖基化,未形成二聚体和多聚体,并具有生物学活性。

In a giving stage after pollination,p53 homologue showed high levels in the antipodal cells,integument,immature endosperm,ovary wall,tracheary elements,and aleurone layer,while c-myc homologue showed low levels in these tissues,only before pollination showed high expression in polar nucleus.

结果发现,在授粉后的一定阶段,在反足细胞、珠被、未成熟的胚乳、子房壁、导管组织和糊粉层中,p53同源基因表达强烈,c-myc同源基因的表达相反,在授粉后的这些组织中基本不表达,而在授粉前的中央细胞的极核中表达水平较高。

CD1a and CD83 molecules could also be expressed on the surface of CD3+ T lymphocytes and CD19+ B lymphocytes. The expression levels of CD1a and CD83 in activated lymphocytes were higher than those in the unactivated lymphocytes, and higher in B lymphocytes than in T lymphocytes.

结果该细胞表达特征性分子CD1a、CD40、CD80、CD83、CD86, CD1a 、CD83在CD3+T、CD19+B淋巴细胞上也表达,在激活的淋巴细胞上表达水平高于未激活的淋巴细胞,而且B淋巴细胞上的表达水平高于T淋巴细胞。

The mc2155 carrying pMind-glmU-AS was induced by different concentration of tetracycline, and GlmU protein was detected in the mc2155 carrying pMind-glmU-AS by Western blotting. The results showed that tetracycline of 20ng/ml has obviously inhibited the growth rate of mc2155 carrying pMind-glmU-AS, but the amount of GlmU protein did not change compared to that of uninduced mc2155 carrying pMind-glmU-AS.

glmU反义RNA的诱导表达与GlmU蛋白的检测分别用5 ng/ml、10 ng/ml和20 ng/ml的四环素诱导携带pMind-glmU-AS表达载体的mc~2155菌株表达反义RNA,绘制细菌生长曲线,结果表明20 ng/ml浓度的四环素可抑制mc~2155/pMind-glmU-AS的生长,但Western Blotting分析结果显示经四环素诱导和未诱导的mc~2155/pMind-glmU-AS中,GlmU蛋白表达量无明显的变化。

No immunostaining was seen in RPE. Double staining that Kir2. 1 and glutamine synthetase, Kir2. 1 and glutamine synthetase colocalized well. Intense Kir7. 1 immunolabeling was present on the apical surface of all RPE cells and appeared to extend over the length of the apical processes. Na〓, K〓-ATPase expression varied among RPE cells, but in highly expressing cells, it co-localized with Kir7. 1. Immunoreactivity of Kir2. 1、Kir4. 1 and Kir7. 1 acomplished by ABC immunohistochemistry in monkey and human retinae got the nearly same results as bovine.

间接免疫荧光组织化学显示,Kir2.1蛋白主要分布在Müller细胞与神经元相接触的细胞膜区域;与Müller细胞的特异性标记蛋白质抗谷氨酸合成酶抗体双重标记显示其分布特性重叠,在RPE未检测Kir2.1蛋白的免疫活性存在;Kir4.1蛋白集中分布于Müller细胞的内侧突起,与GS蛋白双重标记显示其分布特性重叠,RPE未检测Kir4.1蛋白的免疫活性存在;Kir7.1主要分布于RPE游离面及其突起的全长,与特异性标记RPE突起的Ezrin蛋白完全重叠,Na〓-K〓ATP酶在不同部位的RPE表达不同,Na〓-K〓ATP高表达的RPE细胞与Kir7.1的分布特性重叠。

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