未编码的

In the unsterilized soil, the colonization of JMC1402P was affected by the physical and chemical factors of soil, and the aboriginal microbe would compete with it.The safety of releasing JMC1402P to the surrounding was tested, the design was as follows: mixing JMC1402P and R. huakuii JS5A16L, JMC1402P and Pseudomonas fluorescens Pf.X16L2 in filterable membrane, sterilized soil and unsterilized soil.

JMC1402P标记菌株释放环境的安全性研究表明，当JMC1402P与华癸中生根瘤菌JS5A16L、荧光假单胞菌Pf.X16L_2混合培养在微孔滤膜上、灭菌盆装土及田间三个环境中时，未检测到华癸中生根瘤菌JS5A16L和荧光假单胞菌Pf.X16L_2中有gfp基因编码的绿色荧光蛋白的存在，在X-GlucA作用下亦没有带有gusA基因的发光菌落。

The result showed that twocDNA fragments which expressed at high level both in shoot and radicle representedthe gene encoding beta-D-glucosidase; one cDNA fragment expressed specifically inshoot represented the gene encoding mitochondria HSP60; most clones of MF12 andMF17 fragments respectively represented the chloroplast genes encoding prp22 andprp19 proteins which are two components of ribosomal small subunit; while thededuced amino acid sequence from each exceptional one clone was respectivelyhomologous to CDC5 proteins and vesicle-associated membrane proteins; otherthree cDNA fragments expressed preferentially in shoot had no homologue inGenBank.

结果发现2个在胚芽和胚根中表达量都很高的cDNA片段代表的是编码玉米β-D-葡糖苷酶的基因；一个在胚芽中表达而在胚根中不表达的片段代表的是编码线粒体分子伴侣HSP60的基因；片段MFl2的大部分克隆测序结果是与叶绿体基因组中编码核糖体小亚基蛋白prp22的基因同源，但其中有一个克隆测定的cDNA片段序列推测的氨基酸序列与CDC5家族成员有较高的同源性；片段MF17的大部分克隆测序结果与叶绿体基因组中编码核糖体蛋白prp19的基因同源，而有一克隆测定的cDNA片段序列推测的氨基酸序列与参与信号转导的膜结合蛋白VAP27和VAMP有较高的同源性；另有3个优先在胚芽中表达的cDNA片段未查询到同源序列。

With the aid of PDQuest 2-DE software, 11 significant protein dots were found out and then 9 of them are pick out to go through peptide mass fingerprinting based on MALDI TOF MS and NCBI database searching . In all, 5 proteins were identified: Ribulose bisphosphate carboxylase , Nucleic acid-binding protein , Ribosomal protection tetracycline resistance protein , hypothetical protein FG04726.1 [Gibberella zeae PH-1], nitric oxide synthase .

应用PMF技术对这11个具有显著表达差异蛋白点中的9个进行鉴定，得到了5个质谱阳性鉴定结果，其中2个蛋白分别为玉米种属的1，5-二磷酸核酮糖羧化酶／加氧酶和叶绿体的核酸结合蛋白；其它的3个蛋白在玉米蛋白质数据库中未见报道，但与其它物种基因编码的蛋白质具有高度的序列同源性。

We initiated a genetic screen for Drosophila olfactory genes by means of SG18.1-Gal4/UAS system: The result validates our screen as an rapid, effective approach for recovering genes controlling glomerular map patterning; From a misexpression screen of 1,515 P{GS} lines, we identified 23 genes that, when forcibly expressed in the olfactory receptor neurons, disrupted the stereotyped anatomy of the Drosophila antennal lobes; These genes, which have not been shown previously to control olfactory map development, encode novel proteins as well as proteins with known roles in axonal outgrowth and cytoskeletal remodeling.

本文首次利用SG18.1-Gal4／UAS基因筛选系统对果蝇嗅觉基因进行了筛选和鉴定：结果表明，所采用的方法可以快速、有效地分离到果蝇嗅觉相关基因；从1515株果蝇P{GS}品系分离到86株突变体，其中40株有明显表型，从中鉴定了23个基因，这些基因在ORN强迫表达时可以损坏果蝇嗅叶的固有结构；在筛选中发现的一些调节嗅觉图谱发育的基因可以编码新蛋白或编码参与轴突生长和细胞骨架重塑的蛋白；为了便于研究，根据基因的功能和所编码蛋白的特点，我们对分离到的基因进行了分类，即参与轴突引导和突触产生、调节转录、调节细胞骨架和功能未确定或未知的基因；并通过对嗅觉感觉器的检测发现，除了调节转录的基因外，其他基因对触觉器的数量和分布影响不明显。

A simplified LLR ordering scheme employed in uncoded systems and an exact LLR ordering scheme employed in coded systems are proposed.

在未编码情况下给出使用线性检测器近似的对数似然比信息排序方法，在迭代检测解码情况下给出使用解码器反馈的比特后验信息进行对数似然比排序的方法。

Compared with uncoded system, the Turbo-coded optical wireless system improves the performance of optical wireless link because Turbo code compensates losses and noises of link and improves jamming-rejection ca...

比较了基于Turbo码的大气无线光通信系统与未编码大气无线光通信系统，发现Turbo码技术能够很好地补偿和克服各种衰减和噪声，提高了系统的抗干扰能力，使系统的性能得到了很大的提高。

Unsuppressed water reference signal from the volume of interest is relatively unaffected by these artifacts and is collected without phase encoding.

有关体积未抑制的水基准信号受这些膺象的影响不大，因而无需相位编码即可以收集。

Among the existing LDPC codes, some codes have large girths but they have small minimum weights which lead to a poor BER performance.

论文摘要：由于短环和最小码重特性未得到重视时，可编码的不规则准循环LDPC码由规则准循环LDPC码导出可能导致其具有劣化的误码率性能。

We suggest that by adopting other suitable MTSs and addition of a 3' untranslated region (3'-UTR) in the recoded mitochondrial gene may improve ND3 mitochondrial import.

我们建议在重新编码的基因上加上其他适合的粒线体标的序列和加入3'端未转译区域(3'-UTR)，或许能够增进ND3的正确运送。

We found that neither the fusioned nor the non-fusioned forms of Hu-P68 cDNA open reading frame could express efficiently in the E. coli cells; in different prokaryotic expression vectors and under various promotors resulted in no distinctive variations. Deletion of 5'UTR by synthesis adaptors, deletion of 3'UTR or signal peptide sequence by PCR gained no gross improvement. Only the truncated API-I proteins were observed to be highly expressed when the 3'ORF fragment of the Hu-P68 cDNA was fusioned at the 3'end of PAI-I gene.

结果发现无论是以融合形式存在，还是以非融合形式存在，人的P68 cDNA基因在E.coli细胞中都未能获得高效表达：在不同表达载体中，不同启动子对P68 cDNA基因的表达没有显著差异；利用合成Adapter的方法删除5'UTR，及利用PCR技术删除3'UTR和信号肽序列对表达没有明显改善；分别表达被分割的P68 cDNA5'端编码区、中央编码区和3'端编码区均未见有明显表达；当P68 3'端编码区与高效表达蛋白PAI-Ⅰ基因3'末端融合时，仅见有PAI-Ⅰ以截断形式高效表达出来。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。