未结合的

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

In calculated crime of joint crime, the paper discusses on the relationship between collusion without perpetration and calculated crime and points out that the person who only colludes but does not perpetrate belongs to co-offender and usually not belongs to co-principal offender. But we shall not always cognize these offenders to be calculated criminal ,we shall analyze their criminal formations combining the concrete situation .

在共同犯罪预备状态中，笔者讨论了共谋而未实行与共同犯罪预备状态的关系，认为共谋而未实行者属于共同犯罪人，且一般不属于共同犯罪的正犯，但不能简单地以行为人所实行的共谋行为属于犯罪预备行为而一概认定其处于犯罪预备状态，而应结合具体情况具体分析。

Methods The hospital records of 146 patients with acute obstructive purulent cholangitis who due to traditional Chinese medicine therapy and 137 cases without using traditional Chinese medicine therapy were reviewed and analyzed.

对本院1990~2003年间收治的急性化脓性梗阻性胆管炎的患者行中西医结合治疗的146例治疗和未行中西医结合治疗的137例患者的治疗方法和效果进行分析。

This paper combines from the stipulate of joint crime and unaccomplished formation of offence in our criminal law and refers to foreign penal codes and criminal theories , makes some research on how to correctly judge the problem of unaccomplished formation of joint crime in practice.

笔者将结合我国刑法对共同犯罪及犯罪未完成状态的规定，同时参考各国的相关立法及理论界的各种观点，对在实践中如何正确判定共同犯罪的未完成状态问题做出探讨。

(1)The threatments with the lateral-wedged insoles were valued for the unserious varus deformity osteoarthritis.(2) Because the symptoms and signs weren't completely recoveried, the lateral-wedged insole were combined with the other treatments.(3)The femoral-tibial angle didn't decrease between before and after treatment.

(1)足底外侧楔形垫是治疗膝骨性关节炎合并膝内翻的有效方法；(2)治疗组中患膝疼痛及体征大部分未完全缓解，最好是与药物或其它有效的治疗方法结合应用；(3)运用足底外侧楔形垫是治疗膝骨性关节炎合并膝内翻，治疗前后胫骨角未见明显变化。

Continuous cooling transformation curves were plotted for the austenite before and after the compression deformation at 85℃, as a result of an investigation on a weathering steel which was tested on MMS-100 tester by means of dilatometry in combination with Vickers hardmeter.

在MMS-100热力模拟实验机上采用热膨胀法结合硬度测试，对耐候钢进行了未变形和变形后的连续冷却的实验研究，建立了未变形和变形奥氏体的CCT曲线。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

We, in this paper, combined the finite elements analysis with space similar material analogy test, studied the stability of the un-supported enclosing rock section during the comprehensive operation construction of the vertical coal shafts, analyzed the distribution laws of enclosing rock strain and surface radial displacement, hereby studying the un-supported height influence upon the enclosing rock.

本文将有限元分析和空间相似材料模拟试验相结合。对煤矿立井应用混合作业方式施工时未支护段围岩稳定性进行了研究，分析了围岩应力及表面径向位移的分布规律，研究了围岩失稳形式及失稳规律，并由此研究了未支护段高度对围岩稳定性的影响。

All experiment animals lived well with no death or malignant arrhythmia following cell transplantation. Frozen sections of the heart showed a few blue-stained cells under the fluoroscope in the cell transplantation group 4 weeks later. This indicated that stem cells were successfully homing. Using hematoxylin-eosin staining, homing cells were distributed over the infarct zone, with the presence of single or mass. In the model control group, no blue-stained cells were determined.

细胞移植后两组大鼠均存活良好，未出现死亡及恶性心律失常，4周后细胞移植组心脏冰冻切片荧光镜检显示心肌内有少量胞核蓝染的细胞，提示干细胞成功归巢，结合苏木精-伊红染色，归巢细胞位于心肌梗死灶周边区域，呈单个或小团聚集；而模型对照组心肌切片未见胞核蓝染的细胞。

The profile of gel-filtration of liver homogenate showed that zineis the main metal bound with MT in normal mice without giving copper,but copper isthe main metal bound with MT after oral administration of copper acetate.

分离正常小鼠肝脏和给铜后荷瘤小鼠肝脏中MT可见，小鼠未服用铜前肝脏中锌是MT的主要结合金属，而在给铜后肝脏中MT的主要结合金属是铜。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。