未组织的

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

Methods: The sample consisted143 children which were in early permanent dentition of ClassⅡ,Division 1malocclusion .Pre-and post-treatment study models were assessed using PeerAssessment Rating index. According to the outcome of treatment, thesecases were classified into three types as "worse, no different","improved"and"greatly improved". The cephalometric analysis and Model Analysis werepreformed with pretreatment lateral cephalograms and study models for"improved"and "greatly improved"cases."T"–test was assessed to find out thesignificant difference between the extraction and nonextraction, and the resultsincluding age factor were analyzed by stepwise discriminant analysis.

应用PAR 指数对矫治前后的143 副恒牙早期的安氏Ⅱ类1 分类错牙合患者牙牙合模型进行评估分析，按矫治结果等级分为三类A、变坏或无改善，B、改善，C、极大改善，然后将B、C 两类患者矫治前的头影测量片及模型分析数据按拔牙与未拔牙进行成组t 检验的单因素分析，找出两组间有显著差异的软硬组织和牙牙合模型指标，对这些指标合并年龄因素进行逐步判别分析。

Elisabetta Bugianesi, MD, PhD, who was not involved in the study, agrees that nonalcoholic fatty liver disease in particular is an important clinical entity that can be difficult to identify."NAFLD constitutes a special challenge for physicians for several reasons: any distinction between nonprogressive and progressive disease is only based on liver histology, but NAFLD patients are generally asymptomatic invasive procedures are not easy to propose to accept," she told Medscape via email.

未参与此项研究的Elisabetta Bugianesi医师同意非酒精性脂肪肝病是重要的临床表徵，但是却不易鉴别诊断；她以电子邮件向Medscape表示，NAFLD对医师们构成一个特别的挑战，因为在非进行性和进行性的疾病之间的差别，仅可从肝脏组织判断，但是NAFLD病患一般是没有症状的，所以对於侵入式的诊断方式难以接受。

The aim of this study was to investigate the expression of mPGES-2 protein in mouse oviduct during the early pregnancy, pseudopregnancy, and estrous cycle by immunohistochemistry and Western blot. The regulation of mPGES-2 protein in mouse oviducts was also studied under delayed implantation, steroid hormonal treatments and oviductal ligation.

本实验主要利用早期妊娠、假孕、输卵管结扎、发情周期、激素调节以及延迟着床等实验模型，结合免疫组织化学和Western blot 的实验方法，详细研究了mPGES-2 蛋白在小鼠输卵管中的表达与调节。mPGES-2 蛋白主要定位于不同时期的伞部和壶腹部的上皮纤毛细胞中，在伞部和壶腹部肌层以及峡部和宫管连接处的上皮和肌层中未检测到mPGES-2 蛋白的表达。

Their metallurgical structure and the surface morphology of passivating film have been observed by metallomicroscope and SEM.

应用动电位法、三氯化铁浸泡试验方法比较了未钝化和硝酸钝化处理的304和2304不锈钢在Cl-介质中的耐点蚀性能，运用金相显微镜、SEM观察了不锈钢的组织和钝化膜的形貌，对304和2304不锈钢在Cl-介质中不同的耐点蚀行为进行了分析。

For the first time, we found that the EHFV propagated in HUVEC has a potentiating stimulation to ET-1 and PGI〓 secretion , which may be respensible for the increased plasma ET -1 level and PGI〓 concentration in patients with EHF during the early phase of the disease.

我们的研究结果提示，HUVEC对EHFV敏感，EHFV感染未引起HUVEC的细胞病变，但可抑制HUVEC的增殖，这有可能改变EC的基因调节，或低调，如Ⅷ R：Ag合成障碍，引起止血功能异常；或高调，如主要组织相容性复合物Ⅱ型抗原、干扰素β和内皮细胞白细胞粘附因子的表达增加，增强EC的炎症反应。

The results show that from the interface to surface the microstructure of the cladded coating is composed of plane grains,tiny grains and fine dendritic grains. The two main compositions of Ni and Cr homogeneously distribute in the section.

结果表明，熔覆修复层的显微组织从界面往上依次由平面晶、细晶层和中部细小的树枝晶构成，主要元素Ni和Cr在截面内均匀分布，原有Cr层与熔覆层内的Cr元素呈连续状，构件修复区以外的铬层与基体的结合未受激光熔覆的影响。

Preliminary research shows that supplemental vitamin A improves the likelihood that the measles vaccine will provide protection. 6 Vitamin A has, since the 1920s, been the subject of much research into the prevention and treatment of childhood exanthems, particularly measles. 7 This nutrient has a critical role in proper immune function , and there is evidence that supplementation with vitamin A reduces the incidence and severity of, and deaths from, childhood measles. 8 9 The World Health Organization has therefore recommended that children with signs of deficiency receive supplementation with vitamin A. The recommended amounts are 100,000 IU for children younger than one year and 200,000 IU for children older than one year, immediately upon diagnosis, and repeated once the next day and once in one to four weeks.

11初步研究表明，补充维生素A的可能性，提高麻疹疫苗将提供保护。6维生素A已自20世纪20年代，被纳入预防和童年exanthems，特别是麻疹治疗中的许多研究课题。7本养分有在适当的关键作用免疫功能，并有证据表明，补充维生素A减少发病率和严重程度，以及，儿童麻疹死亡。8 9世界卫生组织已因此，建议与不足的迹象儿童得到补充与维生素A的建议金额10万国际单位的儿童未满一年，20万国际单位的儿童一年以上，立即诊断，重复一遍，第二天，一次在1至4周。

Crack reasons for this are: the heating temperature is too high as a result of quenching or cooling in a hurry, the quality of thermal stress and metal volume changes in the organization of the anti-stress than the breaking strength of steel; the work of the original surface defects (such as surface micro-cracks or scratches) or the internal defect of steel (such as slag, serious non-metallic inclusions, white point, the residual cavity, etc.) formed in the quenching stress concentration; serious Decarburization and carbide surface segregation; parts after quenching Back lack of timely or tempering fire; processes in front of the stress caused by excessive cold red, forging folding, turning deep marks, sharp edges and corners, such as the oil groove.

造成这种裂纹的原因有：由于淬火加热温度过高或冷却太急，热应力和金属质量体积变化时的组织应力大于钢材的抗断裂强度；工作表面的原有缺陷或是钢材内部缺陷（如夹渣、严重的非金属夹杂物、白点、缩孔残余等）在淬火时形成应力集中；严重的表面脱碳和碳化物偏析；零件淬火后回火不足或未及时回火；前面工序造成的冷冲应力过大、锻造折叠、深的车削刀痕、油沟尖锐棱角等。

It's regarded as a dentin specific protein, The gene structure and the expression pattern of DSPP at transcript level are well known in recent years, however, the expression pattern of DSPP protein, and it's possible role during tooth development and dental pulp reparation after injury are not clear yet.

近年来，DSPP的基因结构及mRNA水平的表达模式已较清楚。蛋白印迹实验证实小鼠牙胚中有DSPP蛋白的存在。但是，DSPP在蛋白水平的表达模式如何？它的生物学功能和作用机制是什么？它在牙髓损伤修复中的作用又如何？目前国内外均未见报道。这些问题的研究，对我们在该蛋白后基因水平的了解具有重要意义，也为我们深入了解牙本质生物矿化机制、对牙体组织疾病和损伤进行生物性防治提供理论依据。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。