未染色的

Methods Immunohistochemistry of streptavidin-perosidase method was applied in the observation of the expression of TIAF-1 in each group, including tissues obtained from the unaffected part of nephrectomized kidneys with renal cell carcinoma (n=6) and cadaveric donor of normal kidneys (n=8) as the control group, tissues with acute rejection (AR, n=16) and those with chronic rejection (CR, n=28), and in the analysis combined with the number of positive cells of CD3, CD20 and CD68 infiltrated in the interstitium.

采用SP免疫组织化学染色法对6例因肾细胞癌行肾切除的未受肿瘤侵犯的正常肾组织和8例供肾正常肾组织、16例急性排斥反应和28例慢性排斥反应移植肾组织中TIAF-1的表达进行观察，并对间质浸润细胞中CD3、CD20、CD68阳性细胞数进行分析。

Results:(1)Among 40 rats, 36 rats were successfully established and the rate of success is 90 percent;(2)All the successfully established models demonstrated polydipsia, polyuria and the body weight was not increased. 6 rats suffered cataract after 3 months, and 4 rats died in 6 months;(3)There was an approximately 61% loss of retinal ganglion cells in the central retina and the thickness of retina thinner under microscope ( P 0.01 ).(4) Electroscope changes include the thinner of retina, disorganization of the membranous disc of the rod cells and the thickness of basal membrane of vessal.(5)In normal group, 1 month dibetes mellitus and 1 month treatmen group, there was no expression of ERK1/ERK2 on the retina tissues. In 3 month diabetes mellitus group, the expression of ERK1/ERK2 was positive.

结果：①40只建模大鼠中36只建模成功，建模成功率为90%；②建模成功的大鼠都表现出多饮、多尿、消瘦、体重不增的表现，有6只大鼠在3个月后出现白内障，有4只大鼠在喂养接近至6个月时死亡；③HE染色光镜下6个月大鼠后极部视网膜节细胞层细胞数明显减少，减少约61%(P.01)，后极部视网膜明显变薄(P.01)；④电镜观察，视网膜变薄，视杆细胞膜盘紊乱，血管基底膜增厚等表现；⑤正常组、糖尿病组和治疗组1个月大鼠视网膜中未见ERK1/ERK2的表达，糖尿病组视网膜组织中3个月时可见少量表达，ERK1/ERK2表达部位为神经节细胞层和内核层；6个月时表达强阳性，部位表达不仅见于内核层、神经节细胞层，色素上皮层也见表达。

The method is taken not pregnant estrum and pregnant 1 D, 2 D, 3 D, 4 D in the morning 4, D afternoon 4, the small rat uterus of D midnight, 5 D, 6 D, 7 D, 8 D makes section, with immune organization chemical coloring and image analyse a technology to be to STAT3 inside uterus of early pregnant small rat velar expression undertakes detecting.

方法取未孕动情期及孕1 d、2 d、3 d、4 d上午4、d下午4、d午夜、5 d、6 d、7 d、8 d的小鼠子宫做切片，用免疫组织化学染色和图像分析技术对STAT3在早孕小鼠子宫内膜的表达进行检测。

RESULTS ①After HE staining, obvious atheromatous plaque has been formed in the experimental group, but there is no histopathology changes in the control group.

结果 ①经苏木精-伊红染色，显微镜下观察可见模型组大鼠的主动脉有明显的粥样斑块形成，对照组未见明显变化。

Methylene blue-basic magenta staining and electron microscopy Regenerating nerve were arranged in bundles in group A and B, there were minifascicle formation and a large amount of proliferated collagen in between the minifascicled, no cicatrices form, regenerating vasa spread randomly, electron microscopy show a large number of myelinated and unmyelinated fiber arranged in buddies.

亚甲基蓝-碱性品红染色及电镜观察两组均见再生神经纤维呈束状分布，有多个神经束，每一个神经束内见大量密集、粗大的有髓神经纤维，神经束之间充满增生的胶原，未见瘫痕形成，再生血管散在分布。

Compared with control group,the ADM level in the left lung increased(P＜0.001),but the ADM levels in the heart,liver,kidney decreased.The ratio of wet to dry weight of the left lung in experiment group was 6.491±0.248,and 5.937±0.361 in control group,there was no significant difference（P＞0.05）.No alveolar wall thicken,alveolar and mesenchyma edema were found in two groups.

实验组兔左肺的湿/干重量比为6.491±0.248，右肺的湿/干重量比为5.937±0.361，双肺的湿/干重量比差异无显著性（P＞0.05）；HE染色结果显示实验组与对照组均未见肺泡壁增厚、间质及肺泡水肿等改变。

Methods Fourteen mutated vpr fragments were selected from patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products were purified, double-cut by Hind Ⅲ and BamH Ⅰ, and the cut products were legated and transformed into competent cells JM109. The 14 reconstructed plasmids were transfected into Hela cells. Cells with pcDNA vpr-wt, pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell were established.

以14个带有HIV-1vpr基因片段的PcD-NA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vpr基因转染细胞、突变株vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经逆转录多聚酶链式反应检测目的基因转染成功后，Pi染色，用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Methods 14 mutanted vpr fragments selected from Chines patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products purified , double-cut by HindⅢ and BamH and the cut products legated and were into competent cells JM109. The 14 reconstructed plasmids electronically transfected into hela cells, and established cells with pcDNA vprwt 、pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell.

将14个带有HIV-1 vpr基因片段的pcDNA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vp r基因转染细胞、突变株 vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经RT-PCR检测目的基因转染成功后，经Pi染色用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Results: Haemoglobin and haematin were detected in the dentinal tubules of discolored teeth from group A and no evidence of ferric or haemosiderin. Specimens from group B demonstrated a negative response to histochemical tests.

组织化学实验结果显示：变色牙牙本质小管内存在高铁血红素和血红蛋白，缺乏铁离子和含铁血黄素：漂白后的牙本质小管内未见任何红细胞的分解产物。B组变色牙漂白4周后染色均消失，牙齿颜色恢复正常。

These results indicated that HA and PmmA are both good bone graft substitutes for mastoid obliteration causing no structural or functional damage to inner ear and inducing no rejection or inflammatory reaction. PmmA, in particular, can be prepared as the occasion requires and can be solidified in a short time, making itself endurable and mouldable.

内耳连续切片，HE染色，光镜观察：实验耳耳蜗结构，形态正常，毛细胞排列整齐，〓泡内壁对HA和PmmA未呈现任何炎性细胞和巨噬细胞等，表明人造骨对内耳结构和功能无损害，不引起排斥或炎症反应，相容性好，是良好的骨组织代替材料，尤其是骨水泥可临时配制，能在短时间内象水泥一样凝固，具有一定的抗压性及成形性，是一种良好的乳突重建材料。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。