未染色的

Sclerostin expression and distances of each osteocyte to the canal surface and cement line were assessed for all osteonal osteocytes in 636 unremodelled osteons chosen from fields ( approximately 0.5mm diameter) with at least one canal staining for alkaline phosphatase (ALP; a marker of bone formation).

我们评估了对636个未重塑的骨单位中所有的骨单位骨细胞的硬化蛋白表达和每个骨细胞到管道表面和粘合线的距离，这些骨单位从至少一个管道碱性磷酸酶(ALP；骨形成标志物)染色的区域选择（直径大约0.5mm）。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生；HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润，纤维细胞轻度增生，硬膜外神经根内有炎细胞浸润及炎性渗出；特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变；免疫组化染色，给药后四周及八周时，硬膜内外均有Ⅱ型胶原纤维增生；固兰染色B、C、D三组未见有明显脱髓鞘改变，与A组相比无明显异常改变；电镜观察B、C、D三组在给药后的四周及八周时，表现为硬膜外神经根内髓鞘厚薄不一，形状不规则，可见成纤维细胞，神经内膜中可见有巨噬细胞；粗大的有髓神经纤维变化明显，无髓神经纤维受累较轻；八周时电镜下D组改变较B、C两组为轻。

And the repression of NO-induced cell death with Ppbi-1 overexpression in transgenic lines was apparent even at SNP concentrations up to 1mM.In addition work,we found that most of suspension cells treated with 0.5mM SNP can be stained by Sytox green,some PCD morphologic characteristics such as chromatin condensation and margination can be observed.DNA Ladder was also detected with these cells.As to the control and the cell treated with 0.5mMSNP+20μM Est,the above-mentioned characteristics can\'t be detected.

对0.5mM SNP和0.5mM SNP+20μM Est处理的飞廉转基因细胞培养三天后用特异性核染料sytox染色，0.5mMSNP单独处理的细胞大部分核被染色，而且细胞核出现了核凝聚、以及染色质边集等凋亡细胞的特征；提取细胞DNA电泳分析，观察到了DNA LADDER，这说明，该细胞可能已经发生凋亡，而Est诱导Ppbi-1基因表达的细胞均未出现这些凋亡特征。

In the experimental tectum, the expression of GFAP in the right SO was higher than the expression of GFAP in the left SO during 1d-60d after after optic nerve damage. The expression of GFAP in the right SO was familiar with the expression of GFAP in the left SO during 85d after optic nerve damage, but the expression of GFAP in SO in the experimental tectum was higher than that in the normal tectum. The result of GFAP expression in the experimental retina and tectum showed that AStrocyte took part in the optic nerve regeneration and it might play an important role in optic nerve regeneration accidents.

在损伤1d后视网膜的神经纤维层和外核层外缘可见较为深的GFAP染色，在损伤3d后视网膜从内网层经内核层至外网层出现稀疏的垂直于视网膜长轴分布的线条状GFAP染色，随时间推移，伤后5d、7d、14d、28d上述染色进一步加深密集，到伤后60d和85d上述染色减弱，阴性对照的视网膜各层未见GFAP阳性染色；(5)正常鲫鱼视顶盖中在SO层内有较浅的黄棕色线条状GFAP染色，在SFGC层

The unlabled effetorcells and labled target cells were plated together with indicated ratio and incubat-ed at 37℃,5% C〓 humidified atmosphere.After 24 h of incubation,the platedcells were collected and stained with propidium iodide、Death cell percentagein each of the effetor and target cells was measured and analyzed in FACS canflow cytometer.

未标记的效应细胞与标记的靶细胞以一定比例混合，37℃，5%CO〓孵育，24h后收集细胞，碘化丙锭染色，经FACScan检测，分析效应细胞和靶细胞中的死细胞比例。

In situ hybridization was used to observe the expression of the MCP-1 mRNA in the walls of the normal arteries and the aneurysms. Results: by the HE straining the walls of the ruptured aneurysms (10 cases) and unruptured ones (2 cases) were consisted of incrassated intima and connectivum extima.

免疫组化：MCP-1组：正常颅内动脉未见MCP-1阳性细胞，未破裂动脉瘤和破裂动脉瘤的阴性对照未见染色细胞。2例未破裂和10例破裂动脉瘤：MCP-1呈高表达，阳性细胞在动脉瘤壁内呈局灶聚集，间断分布动脉瘤内膜。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色：翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成；基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质，在翼状胬肉体部深层基质中存在粗糙的纤维组织；正常球结膜组织细胞排列整齐；基质为疏松结缔组织，胶原纤维平行排列，其间可见成纤维细胞，散在少量中性粒细胞、毛细血管；Masson染色：翼状胬肉浅层基质中存在致密的胶原纤维染色，深层基质中的胶原纤维存在变性样改变；VG染色：翼状胬肉组织深层基质中存在大量变性的胶原纤维，其间夹杂黑色的弹性纤维；免疫组化染色法：MMP-3在翼状胬肉上皮细胞中呈强表达，成纤维细胞中呈中等强度表达，血管内皮细胞中未见表达；LN在血管壁中呈强表达，在上皮细胞基底膜中呈中等强度表达，在整个基质中未见明显表达；col Ⅲ在整个翼状胬肉基质中呈强表达。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果：用诱导分化液作用后，未见小的、伴有突起的放射状形态的神经细胞；抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性； C2C12细胞β Tubulin Ⅲ抗体染色阴性；上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

Masson's trichrome staining was performed, and it was observed under light microscope that the small white parts in the middle were immature chondrocytes, and there were green collagen around most of the mature chondrocytes.

麦松染色，光镜下观察，中部小部分白色为未成熟的软骨细胞，大部分成熟软骨细胞周围有染成绿色的胶原分泌。

The dyeing fastnesses are identical by microcapsulated and uncapsulated dyes. But the color staining was higher by microcapsulated dyes than uncapsulated dyes.

使用胶囊化的染料与未胶囊化的染料进行热溶染色，织物的染色牢度相同，但前者染色织物的沾色牢度好于后者。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。