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OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast.

结果 透射电镜下观察到OVCs超微结构的改变为核大,核/浆比大,核仁小,胞浆内细胞器少,可见少量内质网和小线粒体,整个细胞呈幼稚、未分化状态;ICAM-1免疫细胞化学检测阳性;损伤肝组织HE染色和ICAM-1免疫组化检测显示,肝损伤初期,Hering管周围OVCs增生并且ICAM-1阳性表达,随着肝损伤的加重,OVCs继续增生,并且,OVCs沿增生的肝纤维向肝小叶迁移,同时,ICAM-1阳性表达也继续增多,并逐渐向肝小叶弥散分布;透射电镜下观察到,肝损伤初期,OVCs在Hering管周围增生,肝纤维随之增生,增生的OVCs与肝纤维黏附生长。

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大,核/浆比大,核仁小,胞浆内细胞器少,可见少量内质网和小线粒体,整个细胞呈幼稚、未分化状态;ICAM-1免疫细胞化学检测阳性;损伤肝组织HE染色和ICAM-1免疫组化检测显示,肝损伤初期,Hering管周围OVCs增生并且ICAM-1阳性表达,随着肝损伤的加重,OVCs继续增生,并且,OVCs沿增生的肝纤维向肝小叶迁移,同时,ICAM-1阳性表达也继续增多,并逐渐向肝小叶弥散分布;透射电镜下观察到,肝损伤初期,OVCs在Hering管周围增生,肝纤维随之增生,增生的OVCs与肝纤维黏附生长。

Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大,核/浆比大,核仁小,胞浆内细胞器少,可见少量内质网和小线粒体,整个细胞呈幼稚、未分化状态;ICAM-1免疫细胞化学检测阳性;损伤肝组织HE染色和ICAM-1免疫组化检测显示,肝损伤初期,Hering管周围OVCs增生并且ICAM-1阳性表达,随着肝损伤的加重,OVCs继续增生,并且,OVCs沿增生的肝纤维向肝小叶迁移,同时,ICAM-1阳性表达也继续增多,并逐渐向肝小叶弥散分布;透射电镜下观察到,肝损伤初期,OVCs在Hering管周围增生,肝纤维随之增生,增生的OVCs与肝纤维黏附生长。

Part three: An experiment study on the repair of peripheral nerve injury by NT-3 gene modified neural stem cells objective To investigate the effective treatment of peripheral nerve injury with NT-3 gene modified neural stem cells, to explore the feasibility of the treatment by means of gene transfection Methods 54 SD rats were divided evenly into 3 groups. The sciatic nerve transected model were made and sutured end to end with epineurium. The neural stem cell without NT-3 gene or with NT-3 gene modified were injected into triceps muscle one time a week, saline injected as control group. The nerve trunks of stomas and triceps muscle were acted as specimen three weeks、six weeks and nine weeks after operation.

最后,为了探讨神经营养素-3基因修饰的神经干细胞对周围神经再生修复的影响,从转基因角度探讨治疗周围神经损伤的有效方法,我们选用54只SD大鼠随机分为3组,造成坐骨神经切断损伤模型,神经外膜端端对位缝合后,于小腿三头肌每周分别注射生理盐水、未被神经营养素-3基因修饰的神经干细胞、及神经营养素-3基因修饰的神经干细胞。

Nano-Fe_3O_4 can induce DNA single-strand breaks without significancy and can not induce micronuclei formation and percentage of apoptosis. Nano-Fe_3O_4 can aggravate the effect of CdCl2 on DNA single-strand breaks and chromosome damage in HepG2 cells. They showed synergistic effects on DNA single-strand breaks after the data were statistic analyzed.

结果发现CdCl2能引起DNA断裂损伤、微核率的增加和细胞凋亡率的增加;nano-Fe_3O_4能引起DNA断裂损伤增加,但无显著意义,未引起微核率和细胞凋亡率的增加;nano-Fe_3O_4能加重CdCl2的对细胞DNA断裂损伤和染色体损伤,且二者联合作用对DNA断裂损伤的结果经统计分析认为有协同作用。

It is unknown, however, what the prevalence of renal damage due to UTI is in SCI patients.

然而,脊髓损伤病患因泌尿道感染造成肾脏损伤的比率仍未明。

This article explains the reoxygenation injury problems in immature myocardial at home and abroad literature,through the reoxygenation injury in cell-biology research and a large number of animal model of test,medical research and clinical research,recognize that the reoxygenation injury is the rootstock of postoperative heart and lung disorders in cyanotic congenital heart disease.

本文就未成熟心肌再氧合损伤的问题结合国内外文献,通过再氧合损伤的细胞生物学研究和大量的动物模型试验研究和医学临床研究,认识到再氧合损伤是发绀型先天性心脏病术后心肺功能紊乱的根源。

The femoral circumflex arteriography of the normal leg was done in 8 patients, and three of them were simultaneously done in a postion of internal rotating of straight leg. Results Comparison with the arteriographic findings in absence of traction, all retinacular arteries but inferior arteries were depicted few branches and less femoral head perfusion in all 9 patients, especially in that of medial femoral circumflex arteriography, as hip joint was tractating in neutral position with the same speed and volume of injecting contrast medium and, the veins were demonstrated poorly or delayed.

结果同一血管部位,同等对比剂注射流率、总量造影,与中立位无牵引状态下造影所见比较,9例患髋中立位牵引时,除外下支持带动脉,所有未损伤的支持带动脉均有不同程度闭塞,相应股骨头颈部灌注不佳或几乎无灌注,静脉显影延迟或显示不清,4例牵引力由3kg增至5kg时,上述改变更为明显;5例患髋维持伸直内旋位时,所有支持带动脉尤其细小分支未见明确显示,股骨头灌注差或未见明确灌注,改变甚于牵引时;2例正常髋内旋位时造影改变类似。

Methods In the first experiment, 48 rabbits were inflicted with a posterior subglottic injury using a diode laser, and there were 10 unwounded controls.

在第一阶段实验中,以二极管激光对48只兔子造成声门下后壁损伤,10只未损伤的作为对照。

P75 expressed only in the injured sciatic nerve,the ipsilateral spinal ganglion and motor neuronal body of spinal cord, not in the normal peripheral nerve system.

P75在坐骨神经损伤一侧的脊髓前角运动神经元胞体、脊神经节感觉神经元胞体和坐骨神经均有表达,而在未损伤的周围神经系统无表达。

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