未处理的

The invention relates to a method for the extraction of keratin from dead animal skins, characterised in the treatment of dead animal skins with a substance of general formula, or at least one corresponding alkali metal, earth alkali metal, ammonium, or phosphonium salt, the variables having the following definitions: R, R = together or independently, H, C6-C14 aryl, C1-C12 alkyl, unsubstituted or substituted with one or several SH or OH groups, R, R = together or independently, H, C6-C14 aryl, C1-C12 alkyl, unsubstituted or substituted with one or several SH or OH groups, whereby at least one of R or R is not H, or R and R are not H and two vicinal groups of R to R together may be C3-C10 alkylene, R = H, C1-C12 alkyl, H-C=O or C1-C4-Alkyl-C=O, X, X, X and X = OH, SH or NHR, whereby where Rto R contains at least one sulphur atom, at least one of X to X = SH and where R to R contains no sulphur atom, at least two of X to X= SH.

本发明涉及一种从死亡动物原皮中除去角质的方法，其特征在于用通式I的物质或至少一种对应的碱金属盐、碱土金属盐、铵盐或∴盐处理死亡动物原皮，式I中各变量定义如下：R 1 和R 4 相同或不同且选自氢、C 6 -C 14 芳基和C 1 -C 12 烷基，其未被取代或被一个或多个SH或OH基团取代；R 2 和R 3 相同或不同且选自氢、C 6 -C 14 芳基和C 1 -C 12 烷基，其未被取代或被一个或多个SH或OH基团取代，其中至少一个基团R 2 或R 3 不为氢，或R 1 和R 4 不为氢，且两个相邻基团R 1 -R 4 可以一起为C 3 -C 10 亚烷基；R 5 选自氢、C 1 -C 12 烷基、H-C＝O或C 1 -C 4 烷基-C＝O；X 1 、X 2 、X 3 和X 4 选自OH、SH和NHR 5 ，其中若R 1 -R 4 含有至少一个硫原子，则至少一个基团X 1 -X 4 为SH，以及若R 1 -R 4 不含硫原子，则至少两个基团X 1 -X 4 为SH。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

analysis of variance, regression, statistic, expected value

当然，现存统计软件掂来即是，比如Mathematic、Statistic等都是很好的数据处理软件，即使Excel本身，除有各种数据处理函数外，还提供了专门的"数据分析"工具包，但这些软件一方面对学生较陌生，另一方面在课堂上直接使用未

BTH and PHC were studied after harvest for inducing disease-resistance of peach fruit inoculated with spore suspensions of Penicillium expansum (1.2x104 CFU/mL).

而乙烯处理未对果实体内的活性氧水平产生明显影响，乙烯处理果实中的CAT、GR和APX活性升高，AsA含量下降。

Abstract］ Objective To Treating the nano-SiO2 particle by silicon coupling reagent, functionalize the SiO2 by making double bond on its surface. Polymerizing on the surface of nano-SiO2 surface by the method called emulsion polymerization, the nano-SiO2 is covered by polymer to get the PMMA-KH-570-nano-SiO2 complex material. Test the surface structure of the surface of nano-SiO2 by FTIR, and test sort of its mechanical performances.Comparing the treated nano-SiO2 which is directly dispended in the PMMA emulsion with the SiO2 without any treatment, we are trying to find an effective method which can not only increase the dispersion of SiO2, but also improve the mechanical performance of the artificial teeth.

目的 通过硅烷偶联剂KH-570处理SiO2纳米粒子，向其表面引入双键使其功能化，采用乳液聚合的方法在SiO2纳米粒子表面进行接枝聚合，实现高分子对SiO2纳米粒子的表面包覆处理，制得PMMA－KH-570－SiO2纳米复合材料，采用FTIR对SiO2纳米粒子表面结构进行了表征研究，并且测定了它的多种机械性能，与表面处理过的SiO2纳米粒子直接分散至PMMA乳液中制得的复合材料、未添加任何增强物质的空白组进行比较，探索一种能够提高SiO2在基体中的分散性，为义齿复合材料的研究和开发提供理论依据和参考。

We didn\'t find activated points in cerebellum and deeper brain.3.Acupuncture of sham point can significantly activated BA2,6,8,13,21,37,40,44,45,47,putamen and other areas.Both the left and right side of the brain have activated points,but points on the right side are more than those on the left.Both sides of middle temporal gyms,parietal lobule,supramarginal gyms and the lentiform nucleus have obvious activated points.The activated points mainly concentrated in the cerebral cortex,the deep-activated are mainly in the putamen.4. Sham needling in sham point can significantly activate BA6, 8,9,10,18,21,37,40,43,44.The activated points are mainly distributed in the right side of the brain.The left side also has some activated points;5.In the comparasion of Acupunture and sham-needling in S J5,we find that BA8 and cerebellum have distinct regional activated points;6.In the comparasion of acupuncture in SJ5 and sham point,we find BA2 and left cerebellar regions have activated points;7.Sham needling in S J5 compared with sham point,we find BA7,8,9,18 and other areas have activated points,the main activated points are at the left brain.It is not difficult to find that the distribution of activated points are mainly in the middle brain,no obvious activated points at the temporal lobe.

结果：通过对数据的处理和分析，我们初步发现：1、外关穴真针刺能显著激活Brodmann area45、37、44、40、22、13、37、47区、海马、杏仁核、黑质等区域，小脑左侧更明显，左侧颞叶皮层激活点多于右侧，且脑部左侧深层激活点多于右侧；2、外关穴假针刺能显著激活BA46、44、41、13、40、37、19区等区域，激活点主要集中表现在大脑皮层，以颞叶为主，小脑及深部未发现明显激活点；3、非穴真针刺组结果分析初步表明，BA2、6、8、13、21、37、40、44、45、47区以及壳核等区域有激活点，大脑左、右侧均有激活点，但右侧更多，双侧颞中回、顶叶下小叶、缘上回及豆状核有明显激活点，激活点主要集中在大脑皮层，深部激活点主要在壳核；4、非穴假针刺能显著激活BA6、8、9、10、18、21、37、40、43、44区等区域有激活点，主要分布在大脑的右侧，左侧也有不少激活点，就其具体分布主要在颞叶和额叶，少部分分布在顶叶和枕叶；5、外关穴真针刺与假针刺对比发现，BA8区和小脑等区域有明显的激活点；6、外关穴真针刺与非穴真针刺对比发现BA2区、左侧小脑等区域有激活点；7、外关穴假针刺与非穴假针刺对比发现，BA7、8、9、18等区有激活点，主要反应点在左侧大脑的枕叶中回、楔叶，顶叶楔前叶及额上回、中回，就其分布不难看出主要在大脑中部，颞叶未见明显激活点。

The growth of unmowed part of a clone did not affected by removing ways in ramet level. But the leave number per tiller was higher in treatments 2, 3, 4 and 7 at 2 months after cutting in tiller level.

在分株层次上，不论刈割方式如何都没有影响无性系未刈割部分构件的生长发育；在分蘖层次上，去除量较少的处理（2，3，4，7）中未刈割部分每分蘖叶数至刈割后2个月时增加较为显著。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

The compatibility of both Gvirens and B.firmus was researched. The fermentation filtrate of Gvirens , under the concentration of 70%, had not distinct inhibitory activity to B.firmus, the biomass of B.firmus would decrease with above the 70% filtrate of Gvirens, but the fermentation filtrate of B.firmus had not inhibitive role to Gvirens. 7, The cooperation control of both Gvirens and B.firm to the pine needle blight was explored:(1) Pathogen inoculation happened prior to the bio-control treatment for 7 days, however, the disease index and control effect had not distinct differences in any treatments even if combination of the two antagonists, but the susceptibility for different kind of pine were different.(2)when antagonist and pathogen were inoculated at the same time, some control effect to the pine needle blight, including certain extent cooperation control of both Gvirens and B.firm were proved, but it wasn't distinct when two antagonists used separately, and the change of the antagonist concentration had little effect to the control effect.

G.virens与B.firmus对松赤枯病的协同控制显示：(1)预先接种病菌后(7天)再进行生物防治处理，不管那种情况，感病指数和防治效果在处理间差异不明显，浓度影响无显著差异，即使是两种生防制剂联合作用也未显著提高其防效，但不同树种间的感病性有明显差异；(2)领抗菌与病原菌同时接种，对松赤枯病有一定防治效果，两菌联合有一定的协同作用，两菌分别单独施用防效差异不显著，浓度变化对防效影响不大；(3)预先接种颇抗菌能显著提高防治效果，两菌联合处理防效更优，感病树种的感病指数可降至13一15，抗病树种的感病指数可控制在3%左右；在同一浓度下B.f1'r功US、叹叮厂即s间无显著差异，在同一领抗菌下，浓度愈高，防效愈高：松，(4)三种生防接种方式的平均效果分析表明，叹F行ens最高平均防效49.5%黑 B。

The strengths of the plain wood rose along with the compression ratio, while the bending strength and MOE lowered as it reached the maximum compression ratio; the plain wood compressed (33%) and heated 10h with 180℃. its bending strength lowered 40%, MOE lost about 50% and hardness lowered 12% than heated 0.5h; the properties not to be compressed after impregnated with PF resin. had a slow rising with the resin concentration, and compressed (33%) after impregnated with PF resin (30%). its bending strength, MOE and hardness increased 82.9%, 98% and 152% respectively.

三倍体毛白杨素材压缩后，其静曲强度和抗弯弹性模量均随压缩率的增大而提高，但接近最大压缩率时均下降；素材压缩（压缩率为33%）后以180℃空气加热处理10 h，静曲强度比同温度压缩保温0.5 h时下降约40%，抗弯弹性模量下降近50%，表面硬度下降约12%；用PF树脂浸渍处理后不经压缩，随PF树脂含量增加木材静曲强度提高幅度不大；用30%PF树脂浸渍后，当压缩率为33%时，其静曲强度、抗弯弹性模量和表面硬度分别比未压缩素材增加82.9%，98%和152%。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。