未切的

From these causes it arose that Marcus, Pertinax, and Alexander, being all men of modest life, lovers of justice, enemies to cruelty, humane, and benignant, came to a sad end except Marcus; he alone lived and died honoured, because he had succeeded to the throne by hereditary title, and owed nothing either to the soldiers or the people; and afterwards, being possessed of many virtues which made him respected, he always kept both orders in their places whilst he lived, and was neither hated nor despised.

由于上述原因，结果马尔科、佩尔蒂纳切和亚历山大这些全是温和谦让的人、正义的热爱者、残暴的敌人、既人道又善良的人却落得个悲惨的下场，唯独马尔科例外。马尔科是生与死都很荣耀的唯一的一个人。这是因为他根据世袭权利继承王位，既不依靠军队也不依靠人民的力量，而且后来，由于他具有许多美德使他受到人们尊敬。当他在世的时候，他一直使军队和人民各安本份，他既没有招人怨恨，亦未引起人们轻视。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Mr. I and plans for half a year want a BB but have not been pregnant this month to the hospital for an examination has been found pregnant as a result of six months but were not pregnant so there is no care to protect their early physical illness is as follows吃了药specific menstruation is the last time I was on the 4th to January 2 (can not recall the specific date that the last two days anyway) cycle for 29-32 days as a result of Kim Jong-January 16 Doctor hospital was opened two days of ofloxacin吊针two bottles a day and oral inflammation hundred net service for three days or not some of the symptoms also had oral Lowe four days after 28 No. Because when cooking large half fingernails cut off after two days due to inflammation of eating cefradine BB always wanted as a result of another failed as Friend introduction has been gradually taking ginseng cream until February 3鹿胎feeling some discomfort to the hospital post-mortem examination urine was very weakly positive on the drug problem at that time ask the director of hospital doctors when she helped me just as long as the operator told me to eat before February 5 in medicine are not all right to ask whether she was in the comfort me?

我和先生计划了半年想要个BB可是一直没有怀上这个月去医院检查验出已怀孕了可是由于大半年都没有怀上所以就没有小心保护自己身体早期因病吃了药具体情形如下我最后一次月经是一月二号还是四号来(具体日期记不起了反正就是这两天)周期为29至32天一月十六号由于泌感到医院看病医生开了两天的氧氟沙星吊针每天两瓶及口服药百炎净服了三天未还是有些症状又口服了洛威四天之后二十八号由于做饭时切掉大半块手指甲后由于发炎吃了两天的头孢拉定另外由于一直想要BB未果经朋友介绍一直在陆续服用人参鹿胎膏直到二月三号感觉有些不适去医院检查验尿呈极弱阳性当时就服药问题问了医院的主任医生当时她帮我算了算告诉我只要在二月五号前吃的药都没什么大碍的请问她是否在安慰我呢？

Chelsea's Brussels-based opponents for Tuesday's game look the weakest link in Group G, which also includes European champions Liverpool and Spanish side Real Betis, having failed to register a point in last season's competition.

X n( K6 Y+{# Q$ J 周二的比赛，切尔西的布鲁塞尔对手看上去是包括欧洲冠军利物浦、西班牙皇家贝蒂斯在内的G组中最弱的一支队伍，安德莱赫特在上赛季的资格赛中一分未得。

The cumulus cells of OCC were cut off and dispersed by 1 mL syringe. The cumulus cells were co-cultured with the immature oocytes retrieved from the COH cycles after they adherent to the bottom of the dish. The immature oocytes were experienced IVM procedures in different culture media. They were divided into 3 groups(the oocytes at germinal vesicle stage from one woman were allotted to the same group randomly). Group 1: basic culture medium+ human follicular fluid; Group 2: solution A+ cumulus cells; Group 3: solution A+ OCC+ follicle stimulating hormone + epidermal growth factor. Then, the maturation rate, fertilization rate and formation rate of available embryo were observed.

在控制性促排卵周期有未成熟卵母细胞时，将同周期成熟卵丘复合体切出部分卵丘细胞，用1 mL注射器抽打分散细胞，贴壁培养。113个治疗周期中，298枚生发泡期卵母细胞经3种不同培养液体外成熟培养(同一病人的生发泡期卵被随机分到某同一组中)：第1组28个周期中73枚：基础培养液+卵泡液；第2组40个周期中115枚：A液+分散贴壁的卵丘细胞；第3组45个周期中110枚：A液+分散贴壁的卵丘细胞+促卵泡生成激素+表皮生长因子。

GELSENKIRCHEN - Captain Paolo Maldini was present with coach Carlo Ancelotti at the press conference ahead of the Schalke 04:'It has been important that in the last two games we have not conceded a goal even if we haven't been on top of our game in defence.

盖尔森基兴—在迎接沙尔克04的挑战之即，队长保罗。马尔蒂尼陪同教练卡洛尔-安切洛蒂一起出席了赛前新闻发布会：在过去的两场比赛中我们一球未失，这点非常重要，虽然我们的防守尚未处于最佳状态。

The two were in the experiment, subjects were from Rakai, Uganda, and Kisumu, Kenya's 7,780 HIV-negative men, the researchers found that when having sex with women, received surgery Circumcision of male than not the recipient of the risk of HIV infection at least 51 percent lower.

在这两项随机进行的实验中，受测者包括来自拉卡伊、乌干达、基苏木和肯尼亚的7780名HIV阴性的男性，研究人员发现，当与女性有性行为时，接受过外科包皮环切术的男性比未接受者受HIV感染的几率至少要低51%。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。