未分选的

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

In single-cell transplants, an average of 27% of unselected melanoma cells from four different patients formed tumours.

在单细胞移植中，来自四个不同患者未分选的黑色素瘤细胞中平均27 ％会形成肿瘤。

The prolif- erative ability of CD133 + tumor cells in vitro was estimated by MTT assay, and the proliferative ability of CD133 +, CD133 - and control SUNE cells were statistically compared. Finally, the immunocytochemical staining method and flow cytometry were used to detect differen- tial ability of CD133 + tumor cells in vitro .

采用免疫荧光细胞化学技术及流式细胞仪检测鼻咽癌细胞株SUNE中CD133的表达情况以及CD133+细胞体外分化能力；免疫磁珠分选技术纯化CD133+肿瘤细胞；MTT法检测CD133+细胞的体外增殖能力，并将其与CD133-及未分选细胞进行比较。

Three types of keratinocytes were studied: the SP, the main population, and the unsorted initial population.

本研究中研究了三群细胞：侧群细胞、主群细胞、以及未分选的细胞群。

Of CBMC can be purified by Mini-MACS as CD34〓 stem cells. B. The number of CD34〓 stem cells can expand to 40.24±9. 86 fold after 14 days. C. No matter in the expression of CD1a, CD80, CD86, and HLA-DR, or in the function of stimulating xenogenous lymphocyte proliferation, there was no difference between CD34+ stem cell DCs or monocyte DCs. D. The percentage of CD3〓CD56〓 cells is the same in CIK cells co-culture with DCs transfected with SKOV3 RNA, CIK cells co-culture with DCs, and CIK cells. E. The expansion rate of CIK cells can be accelerated by co-culturing with loaded or unloaded DCs. However, the expansion rate between loaded or unloaded group is the same. F. The strongest cytotoxicity against SKOV3 cell line was achieved by CIK cells co-cultured with DCs loaded with SKOV3 RNA.

结果：1、Mini-MACS分选系统可自CBMC中提取0.78±0.31%的CD34〓细胞；2、体外培养14天后可获得原始CD34〓细胞量40.24±9.86倍的细胞；3、不论在CD1a、CD80、CD86及HLA-DR的表达上，或是刺激异体淋巴细胞增生的功能上，脐带血CD34〓细胞与单核细胞来源的DC都没有差别；4、CIK细胞中CD3〓CD56〓双阳性的表达率在与RNA转染DC共培养的CIK细胞组、与DC共培养的CIK细胞组及单纯CIK细胞组3组间比较无差异；5、脐带血CIK细胞增殖显著，培养14天时可扩增18.18±5.59倍，培养21天时可扩增35.02±6.30倍；5、与未转染或转染DC共培养的CIK细胞在培养第14天后增殖速率大于单纯CIK细胞。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。