未分离的

An experiment was performed to compare effect of different cell media on embryonic germ cells derived from primordial germ ridge of Kunming albinotic strain mouse.

以昆明白品系小鼠胎儿生殖嵴为材料，以不同的培养液分离培养胚胎生殖细胞，发现小鼠胎儿肝细胞条件培养液的效果好于未添加白血病抑制因子的基础培养液，而差于添加了1 000 IU/mL LIF的基础培养液。

Both our patients presented with an acute onset of facial diplegia accompanied by other mild symptoms such as diplopia, dysphagia, and neck and limb weakness. The presence of hyperreflexia confused us, and in both patients, cerebral disease was initially suspected. Brain MRI was unremarkable, but electrophysiologic studies indicated peripheral involvement of the facial nerves.

但2例患者进一步的脑部MRI检查均未见异常，电生理检查却均提示周围面神经受累；况且，2例患者的一些临床特点均另我们考虑到格林-巴利综合征的可能：患者1有前驱水样便病史，而患者2的脑脊液则表现出蛋白细胞分离现象。

The nitrate non-utilizing mutantas an another added genetic marker was used to select the out-crossed perithecium from self-crossed perithecia. Seven crosses were tested in all, including cross between S×S, MR ×S, MR×MR,HR ×S, as well as HR ×MR. The results showed that no recombinant phenotype was found among all progeny of seven crosses. Progeny segregation of crosses between the parents with different sensitive levels (i.e. MR×S, HR×S,HR×MR) clearly fit a 1:1 ratio of two parental phenotypes, and no segregation was found in the crosses of S×S and MR × MR.

从25个敏感菌株和31个抗性菌株中随机挑选了2个S、3个MR和1个HR，并以硝酸盐营养缺陷型突变体作为另一个遗传标记，按照S×S、MR×MR、MR×S、HR×S、HR×MR等共设计了7个杂交组合，对各杂交后代对多菌灵的敏感性测试发现，在所有杂交后代中均未出现除双亲表现型以外的重组型个体，MR×S、HR×S及HR×MR的杂交后代出现了1∶1的分离比例。

The N-termini of GspGHIJ are highly homologous to the N-terminus of PilA pilus, and were considered as the same family. However, the pili structure of GspGHIJ structure had never been identified or isolated.

在这些基因蛋白中由於GspGHIJ的NH2-端具有与PilA纤毛的NH2-端相当高的同源性，因此这些蛋白被认为是属於同一家族，可是GspGHIJ的纤毛结构一直未被鉴定、分离出。

By using high performance liquid chromatographycombined withthin-layer chromatography,three PG:salicin,salicortin and deltoidin weredetected in the bark of P.deltioides,Five kinds of PG were detected in the leaves,which included trmuloidin and tremulacin besides the three PG.

采用薄层色谱与高效液相色谱法相结合的方法，在实验材料的树皮中测定到三种主要的酚甙类物质：水杨甙、柳皮甙和美洲黑杨甙，在叶片中测定到5种酚甙类物质，除上述3种物质外，还包括2′-苯甲酰水杨甙和颤杨甙，对于后两者在前人的研究中未见报道。研究还对5种化学物质进行了分离。

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

In order to supply basic thermodynamic data for the separation design of acetyl acetone from the isomerization reaction of isopropenyl acetate,vapor-liquid equilibrium data of isopropenyl acetate-acetyl acetone system under 101.3 kPa was measured using Ellis equilibrium still.

为了给乙酸异丙烯酯异构化生产乙酰丙酮工艺的产物分离设计提供基础热力学数据，用埃利斯平衡蒸馏器测定了101.3 kPa下乙酸异丙烯酯-乙酰丙酮体系的汽液平衡数据，结果表明，该二元体系未形成共沸物，实验数据通过了Herrington面积法的热力学一致性检验。

Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法：穿刺抽取15 mL脐带血，密度梯度法分离吸取分层界面的白色环形絮状物，其中富含单个核细胞，离心后沉积细胞用含15％胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮，调整细胞密度为1×109 L-1接种于25 T培养瓶内，放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养，72 h后更换培养基，去除红细胞及其他未贴壁细胞，至70％~80％融合时传代。

In the New York cluster, all three patients received combined spinal-epidural anesthesia from the same anesthesiologist who was masked at the time of the procedures and from whom S. salivarius was not subsequently isolated, based on the results of an investigation by the hospital and the state department of health, the authors wrote, noting also that samples of the anesthetic medication were negative for bacteria by culture and 16S ribosomal DNA sequence analysis.

在纽约州的群体中，所有3例患者均接受由同一名麻醉师进行的联合脊髓硬膜外麻醉操作，根据其所在医院和州卫生部的调查结果，在进行操作时该麻醉师佩戴了面具，并且后来未从其身上分离出唾液链球菌，作者写道，并指出麻醉剂样品培养和16S核糖体DNA序列分析均显示细菌阴性。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。