未免疫的

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

Results Analysis of the expression of proteins of JAK2 in leukemic blasts, there was no detectable constitutive expression of phosphorylated JAK2 in all the AML samples analyzed; Furthermore, we could detect a constitutive activation of STAT 3 by immunoprecipitation of the targeted protein in primary leukemic blasts from 3 of 11 AML patients without any cytokine treatment; Pretreatment clinical characteristics, and treatment regimens did not differ significantly between patients with and without constitutive STAT3 activity, there was not significant relation between the constitutive activation of STAT3 and the FAB subvariety and chromosome karyotype; However, the 3 patients with constitutive STAT3 activity seemed to have adverse treatment outcome, one did not show remission, the other two patients relapsed shortly after remission; Consistent with the above-mentioned result, leukemic blasts from the 3 samples with constitutive expression of STAT3 showed higher percentage of spontaneous apoptosis and S-phase cells.

在实验研究基础上，本研究进一步运用免疫沉淀和Western Blot印迹方法，检测AML患者原代细胞JAK2和STAT3活化情况，并分析了STAT3活化与AML患者预后有关指标改变的关系。结果我们的初步研究发现，11例AML患者中，有3例呈现STAT3组成型活化（STAT3阳性，下同），无一例呈JAK2组成型活化。上述3例STAT3组成型活化与FAB分型和染色体核型改变未见明显相关性；在此3例患者中，1例未取得缓解，另外2例虽然取得短暂缓解，但均在二个月内复发。上述3例STAT3阳性AML患者S期白血病细胞百分率数值较组高，而凋亡率数值较低。

Serum levels of Ang-2 and VEGF in 24 de novo, 18 complete remitted, 7 unremitted, 13 relapsed patients with AL were measured by enzyme-linked immunosorbent assay, and compared with those of normal controls.

应用酶联免疫吸附试验测定24例AL初发未治，18例完全缓解，7例未缓解，13例复发患者血清中Ang-2和VEGF的含量，并与正常对照组比较。

But there are few or no visible F-actin ring of blocking proteinase being released in MC of tryptase and chymase immunoreactivity MC(subscript TC and MC of chymase immunoreactivity MC(subscript C. Cell appearance was inflated and intracellular proteinase had been released.

而类胰蛋白酶和类糜蛋白酶免疫反应性的肥大细胞及类糜蛋白酶免疫反应性的肥大细胞内少见或未见明显的阻碍蛋白酶释放的丝状肌动蛋白环；细胞形态膨胀，细胞内蛋白酶已释放。

Results among the 104 primary acute leukemia patients, the number of patients with t lymphocyte leukemia,b lymphocyte leukemia,acute myeloblastic leukemia,mixed phenotype acute leukemia and umclassified leukemia were 4(3.8％),38(36.5％),58(55.8％),2(1.9％),and 2(1.9％),respectively.conclusion detecting immune marker on leukemic cell by abc-ap two step immune method is very simple which can be finished with a light microscope,and it can provide a reliable basis for the diagnosis and classification of leukemia and even the targeting therapy for leukemia.

结果 104例初发急性白血病中，t淋巴细胞白血病4例(3.8％)，b淋巴细胞白血病38例(36.5％)，急性髓细胞白血病58例(55.8％)，急性混合性白血病2例(1.9％)，未行明确分类的2例(1.9％)。结论 abc-ap免疫二步法检测白血病细胞免疫标记方法简单，只须普通光学显微镜就能开展实验，可为白血病的诊断、分型、分类乃至白血病靶向治疗提供可靠依据。

The prolif- erative ability of CD133 + tumor cells in vitro was estimated by MTT assay, and the proliferative ability of CD133 +, CD133 - and control SUNE cells were statistically compared. Finally, the immunocytochemical staining method and flow cytometry were used to detect differen- tial ability of CD133 + tumor cells in vitro .

采用免疫荧光细胞化学技术及流式细胞仪检测鼻咽癌细胞株SUNE中CD133的表达情况以及CD133+细胞体外分化能力；免疫磁珠分选技术纯化CD133+肿瘤细胞；MTT法检测CD133+细胞的体外增殖能力，并将其与CD133-及未分选细胞进行比较。

Keywords: symbiontic evolution, immune modulation, antibody chrome, neural evolution

通过对神经元群体而不是神经网络群体进行进化设计，显著地减轻了计算量，同时利用生物免疫原理中的浓度机制和个体多样性保持策略进行免疫调节，有效地克服了未成熟收敛现象，提高了群体的多样性，从而加快优化设计速度。

Immunostimulatory sequence (155), unmethylated CpG dinucleotides in particular base contexts, are relatively common in bacterial DNA but rare in vertebrate DNA, and can be detected as an infectious danger signal by the vertebrate innate immune system.

DNA免疫激活序列（immunostimulator sequence，ISS）是发现于细菌DNA 中的一些以未甲基化的 CpG二核苷酸对为核心的具有免疫激活功能的核苷酸序列。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。