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Northern blot analysis of iNOS expression and iNOS stability revealed that bismuth subgallate (100 μM) significantly reduced the amount of iNOS mRNA and increased iNOS mRNA degradation. Because expression of iNOS is mediated by transcription factor, NF-κB, in the transcriptional level and immunosuppressor, TGF-β, at the post-transcriptional level, we examined the effect of bismuth subgallate on TGF-β1 production and NF-κB activity.

在本实验中,我们发现仅bismuth subgallate会减少以interferon-γ and lipopolysaccharide活化之RAW 264.7 巨噬细胞株中一氧化氮之含量,我们进一步以北方墨点分析iNOS mRNA含量及iNOS mRNA稳定性,结果显示处理bismuth subgallate的活化巨噬细胞中,iNOS mRNA含量较处理之细胞明显地减少约60%,其iNOS mRNA半衰期较处理之细胞明显地减少约50%。

Those who work for the Lord today must first be made pure through the substitutionary atonement of Jesus Christ.

到三十岁(4︰3)才真正开始服事。到五十岁时,他们要从这份辛苦的工作上退下来,但仍然可以协助较年轻的利人;当然,那些经验丰富、年长又有智慧的利人可以担任顾问和教师,并继续看守会幕内的圣物。

Ching-man hopes to be assigned with a public housing unit and that her application for family reunion will be approved soon - in the meantime, her life is suffocatingly difficult.

她申请公屋四年,但因居港七年,仍获分配公屋,她最盼望是尽快分配公屋及子女快些从内地批准来港团聚。

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml,分离单个核细胞体外诱导培养DC,并将实验组和对照组细胞各分为三组,A组为处理组,B组为冻融组,C组为RNA转染组。A组DC在培养5d后,每孔加入TNF-α200U/ml;B组DC培养第5d后,每孔中加入癌细胞蛋白抗原,使DC/肿瘤细胞=1:1,继续培养4h后,每孔加入TNF-α200U/ml;C组将培养至第5d的成熟DC用肿瘤细胞RNA进行转染(DC:RNA=1×105/ml: 5ug,按照invitrogen DMRIE-C试剂说明书进行操作),转染结束后用完全培基取代转染剂,每孔加入TNF-α200U/ml。

Hematoma extended over a mean of 1.9 levels. Maximal thecal sac compression due to hematoma occurred at an adjacent, nondecompressed level in 28% of patients.

血肿范围平均1.9个节段。28%患者的临近减压节段发生血肿从而导致硬膜受压。

Methods Immunohistochemistry was used to detect Tiam 1 expression in 89 cases of primary tumor and metastatic lymph node of thyroid carcinoma.

结果淋巴结转移癌组织中Tiam1的表达显著高于甲状腺癌原发灶中的表达(P.05);伴发转移的甲状腺癌组织比发生转移的甲状腺癌组织的Tiam1表达明显增强(P.05);甲状腺髓样癌、分化癌组织中Tiam1的表达显著高于乳头状癌、滤泡癌中的表达(P.05);高临床分期甲状腺癌组织中的Tiam1表达明显高于低临床分期中的表达(P.05)。

A cDNA library with a tittering of 7+106 pfu/mL was constructed basing on the cotton boll shell (10 days post anthesis, dpa) RNA obtained above. The recombinant ratio was found to be ca. 90% by blue-white counting.

首次构建了构建了花后10天棉铃壳cDNA文库,稀释文库滴度达到7×10~6,蓝白斑计数计算重组率为90%,为棉铃发育基因及其启动子的克隆研究打下了基础,目前国内外见类似报道。

The transactor thinks activating the atomic interaction in the intact reactor is practicable.

办理人认为激活受影响的反应堆内原子的相互作用办理人认为激活受影响的反应堆内原子的相互作用是种可行的。

In the absence of lumicrine factors, all four segments regressed to a transcriptionally undifferentiated state, consistent with the less differentiated histology.

在lumicrine因子的缺失下,附睾4个区域的转录水平回退到分化阶段,与组织学上的分化一致。

Compared with the unactivated P polyelectrolyte film, the activated form P/PMMA polyelectrolyte film obtained by adopting the technology of the invention has better absorbency, room-temperature conductivity, electrochemical stability and the interface stability of electrolyte and lithium electrode; the performance of a lithium secondary battery prepared by using activated electrolyte is obviously better than the performance of the lithium secondary battery prepared by using the unactivated polyelectrolyte; the invention has simple technology, short reaction time, and convenient and easy condition for technologized production.

采用本发明技术获得的活化型P/PMMA聚合物电解质薄膜相比活化的P聚合物电解质膜,有更好的吸液性和室温电导率,更好的电化学稳定性及电解质和锂电极的界面稳定性;用活化后的电解质制备的锂二次电池性能明显好于活化聚合物电解质制备的锂二次电池;本发明工艺简单,反应时间短,为工艺化生产提供了简便易行的条件。

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推荐网络例句

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。